Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,276,215	C→A	R362L (CGC→CTC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,276,215	0	C	A	100.0%	139.5 / NA	42	R362L (CGC→CTC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/26);  total (16/26)

GAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACG  >  CP009273/4276153‑4276279                                                               |                                                                 gaATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATc                                                             >  1:5592885/1‑68 (MQ=255)   aTGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGc                                                           >  1:1631313/1‑68 (MQ=255)   aTGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGc                                                           >  1:4226957/1‑68 (MQ=255)   aTGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCAACAGCGCGAGGATCGc                                                           >  1:323046/1‑68 (MQ=255)    tGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGcc                                                          <  1:4495868/68‑1 (MQ=255)    tGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGcc                                                          <  1:393694/68‑1 (MQ=255)      cgcAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGt                                                        >  1:654704/1‑68 (MQ=255)        cAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTgg                                                      >  1:1581146/1‑68 (MQ=255)            gacgaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGc                                                  >  1:3909417/1‑68 (MQ=255)              cgaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCgg                                                <  1:552543/68‑1 (MQ=255)               gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:1029641/68‑1 (MQ=255)               gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:548811/68‑1 (MQ=255)               gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:4632237/68‑1 (MQ=255)               gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:4222624/68‑1 (MQ=255)               gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:2709601/68‑1 (MQ=255)               gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:2320275/68‑1 (MQ=255)               gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:3839805/68‑1 (MQ=255)               gaACGGGCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                               <  1:3283031/68‑1 (MQ=255)                 aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                             <  1:5262274/68‑1 (MQ=255)                 aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                             <  1:4149021/68‑1 (MQ=255)                 aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                             <  1:2732329/68‑1 (MQ=255)                 aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                             >  1:1364697/1‑68 (MQ=255)                 aCGGTCGGTGCCTTCGAGCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                             <  1:5267181/68‑1 (MQ=255)                   ggtcggtGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATag                                           >  1:4395750/1‑68 (MQ=255)                   ggtcggtGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATag                                           <  1:4567665/68‑1 (MQ=255)                   ggtcggtGCCTTCGATCGCTTTATCGCCTTCAGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATag                                           <  1:3041565/68‑1 (MQ=255)                        gtGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAat                                      <  1:450708/68‑1 (MQ=255)                         tGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGGGCGGTATAGAGAata                                     <  1:5050199/68‑1 (MQ=255)                          gCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAatat                                    >  1:4770572/1‑68 (MQ=255)                             ttCGATCGCTTTATTGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGa                                 >  1:773575/1‑68 (MQ=255)                             ttCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGa                                 <  1:2027717/68‑1 (MQ=255)                              tCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGAc                                <  1:5020565/68‑1 (MQ=255)                                   cGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGAt                           <  1:392048/68‑1 (MQ=255)                                    gCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATg                          <  1:4051548/68‑1 (MQ=255)                                      tttATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCt                        <  1:5258657/68‑1 (MQ=255)                                          tCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTc                    <  1:5066359/68‑1 (MQ=255)                                                tCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCcaccac              >  1:965697/1‑68 (MQ=255)                                                 ccGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCaccacc             >  1:714485/1‑68 (MQ=255)                                                    ccATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTgc          >  1:447196/1‑68 (MQ=255)                                                       tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc       <  1:4171251/68‑1 (MQ=255)                                                       tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc       >  1:631343/1‑68 (MQ=255)                                                       tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc       >  1:1252489/1‑68 (MQ=255)                                                           cgcgagGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATAc   <  1:1819299/68‑1 (MQ=255)                                                            gcgagGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACg  <  1:2594968/68‑1 (MQ=255)                                                               |                                                                 GAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACG  >  CP009273/4276153‑4276279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑