Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,629,517	C→T	V203M (GTG→ATG)	arcA ←	response regulator in two‑component regulatory system with ArcB or CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,629,517	0	C	T	100.0%	115.9 / NA	34	V203M (GTG→ATG)	arcA	response regulator in two‑component regulatory system with ArcB or CpxA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/28);  total (6/28)

TGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGG  >  CP009273/4629454‑4629581                                                                |                                                                 gggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCt                                                              <  1:821744/67‑1 (MQ=255)  ggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTa                                                             >  1:5162157/1‑68 (MQ=255)    cGATGATTTCCTGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa                                                           <  1:1326223/68‑1 (MQ=255)    cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa                                                           <  1:1824156/68‑1 (MQ=255)    cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa                                                           <  1:4310655/68‑1 (MQ=255)    cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa                                                           <  1:4241379/68‑1 (MQ=255)    cGATGATTTCCGGCGTATCCGCCGTAGAGGCGAAATGTTTACGAATACGGCGGATCGTCATGTCTCCa                                                           <  1:2915953/68‑1 (MQ=255)           ttCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGt                                                    <  1:4958237/68‑1 (MQ=255)               ggcgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTg                                                >  1:1664231/1‑68 (MQ=255)                 cgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCg                                              >  1:5367457/1‑68 (MQ=255)                  gtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGGCGTGCgg                                             <  1:1428584/68‑1 (MQ=255)                   tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt                                            <  1:2306916/68‑1 (MQ=255)                   tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt                                            <  1:5652449/68‑1 (MQ=255)                   tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt                                            <  1:2757231/68‑1 (MQ=255)                   tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt                                            <  1:3388253/68‑1 (MQ=255)                   tatccggcgtaGAATCGAAATGGTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt                                            <  1:1226920/68‑1 (MQ=255)                         gcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc                                      <  1:4716611/68‑1 (MQ=255)                                ttCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGc                               <  1:617964/68‑1 (MQ=255)                                 tCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGcc                              <  1:5354484/68‑1 (MQ=255)                                     aaTGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTc                          >  1:1959040/1‑68 (MQ=255)                                       tGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAt                        <  1:1903057/68‑1 (MQ=255)                                       tGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAt                        <  1:44178/68‑1 (MQ=255)                                          ttACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAtttt                     <  1:4335096/68‑1 (MQ=255)                                          ttACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAtttt                     <  1:4324925/68‑1 (MQ=255)                                          ttACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAtttt                     <  1:5471267/68‑1 (MQ=255)                                            aCGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGATTCAGCTCACGGCCGGTCATTttct                   <  1:4982798/68‑1 (MQ=255)                                               aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca                <  1:4569810/68‑1 (MQ=255)                                               aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca                <  1:3179102/68‑1 (MQ=255)                                               aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca                >  1:2442927/1‑68 (MQ=255)                                               aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca                <  1:3304893/68‑1 (MQ=255)                                               aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca                >  1:3919037/1‑68 (MQ=255)                                                    ggcggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGt           <  1:2811065/68‑1 (MQ=255)                                                    ggcggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGt           <  1:2532978/68‑1 (MQ=255)                                                             tCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACgg  <  1:4555091/68‑1 (MQ=255)                                                                |                                                                 TGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGG  >  CP009273/4629454‑4629581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑