Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	155,132	C→G	R161P (CGC→CCC)	pcnB ←	poly(A) polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	155,132	0	C	G	100.0%	109.2 / NA	33	R161P (CGC→CCC)	pcnB	poly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (21/12);  total (21/12)

ATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCC  >  CP009273/155077‑155195                                                        |                                                                aTCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGt                                                     <  1:4271908/68‑1 (MQ=255)   cACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtct                                                   <  1:1907431/68‑1 (MQ=255)   cACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtct                                                   >  1:2544165/1‑68 (MQ=255)    acggacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtctt                                                  >  1:3613094/1‑68 (MQ=255)    acggacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtctt                                                  <  1:3005963/68‑1 (MQ=255)    acggacggGAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtctt                                                  >  1:4500258/1‑68 (MQ=255)      ggacggTAAAATCCGCTACGCTGTAGTACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtcttct                                                >  1:1603748/1‑68 (MQ=255)                  ccGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCg                                    >  1:1790640/1‑68 (MQ=255)                  ccGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCg                                    >  1:4195415/1‑68 (MQ=255)                  ccGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCg                                    <  1:3151025/68‑1 (MQ=255)                     cTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGaaa                                 <  1:2911381/68‑1 (MQ=255)                       aCGCTGTAATACAGGCTGTTGATAGTTAAATCGGGTCGCTGGGCGTCTTCTTCGATGGAGCCGAAAAt                               <  1:3541017/68‑1 (MQ=255)                         gCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTTGGCGTCTTCTTCGATGGAGCCGAAAAtgt                             >  1:1085519/1‑68 (MQ=255)                              aaTACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcg                        >  1:3986781/1‑68 (MQ=255)                              aaTACAGGCTGTGGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcg                        >  1:4401815/1‑68 (MQ=255)                               aTACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcgc                       <  1:5650016/68‑1 (MQ=255)                                tACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcgcg                      <  1:4907233/68‑1 (MQ=255)                                tACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcgcg                      <  1:1795750/68‑1 (MQ=255)                                 aCAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                     >  1:2540249/1‑68 (MQ=255)                                 aCAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                     >  1:3742384/1‑68 (MQ=255)                                 aCAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                     >  1:535971/1‑68 (MQ=255)                                  cAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgca                    <  1:1799865/68‑1 (MQ=255)                                   aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag                   >  1:5242488/1‑68 (MQ=255)                                   aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag                   >  1:3572475/1‑68 (MQ=255)                                   aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag                   >  1:444238/1‑68 (MQ=255)                                   aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag                   >  1:4431047/1‑68 (MQ=255)                                   aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTGCGATGGAGCCGAAAATGTTGTCGCgcag                   >  1:4086725/1‑68 (MQ=255)                                        gTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACa              <  1:1990212/68‑1 (MQ=255)                                         ttGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACAt             <  1:4403989/68‑1 (MQ=255)                                           gATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGc           >  1:156062/1‑68 (MQ=255)                                               gTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGtt       >  1:3454831/1‑68 (MQ=255)                                               gTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGtt       >  1:5182730/1‑68 (MQ=255)                                                    aTCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGcc  >  1:182302/1‑68 (MQ=255)                                                        |                                                                ATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCC  >  CP009273/155077‑155195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑