Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	829,599	G→T	M358I (ATG→ATT)	dinG →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	829,599	0	G	T	100.0%	127.7 / NA	39	M358I (ATG→ATT)	dinG	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/29);  total (10/29)

GCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATT  >  CP009273/829534‑829662                                                                  |                                                                gCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttct                                                               >  1:4517543/1‑68 (MQ=255)   gATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttctgc                                                             >  1:1052758/1‑68 (MQ=255)   gATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttctgc                                                             >  1:3803290/1‑68 (MQ=255)   gATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttctgc                                                             >  1:1694541/1‑68 (MQ=255)      gggCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTg                                                          <  1:1106342/68‑1 (MQ=255)        gccaaCTGCCAGATGAAGTGCTGGAGATCTGCAAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGc                                                        <  1:1051054/65‑1 (MQ=255)              tGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCg                                                  >  1:1662424/1‑68 (MQ=255)               gCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCgg                                                 >  1:4294965/1‑68 (MQ=255)                ccAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGa                                                <  1:3531186/68‑1 (MQ=255)                ccAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGa                                                <  1:954149/68‑1 (MQ=255)                ccAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGa                                                <  1:597167/68‑1 (MQ=255)                 cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg                                               <  1:3804905/68‑1 (MQ=255)                  aGATGTAGTGCTTGAGATCTGCCAGCGGCTGGCAAATCTCTCGGGGATTCTGCGTGGCCTGGCGGAGt                                              <  1:4376112/68‑1 (MQ=255)                    aTGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTa                                            >  1:4674899/1‑68 (MQ=255)                       aaGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTAttt                                         <  1:1308328/68‑1 (MQ=255)                           gCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTa                                     >  1:4754568/1‑68 (MQ=255)                           gCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTa                                     >  1:137351/1‑68 (MQ=255)                              ggAGATCTGCCAGCGGCTGGCAAAACTCACCGACATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACg                                  <  1:828803/68‑1 (MQ=255)                                agaTCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGAt                                <  1:240140/68‑1 (MQ=255)                                agaTCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGAt                                <  1:1534127/68‑1 (MQ=255)                                     tGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTCGCCTGGCGGAGTTATTTCTTAACGATTTAAg                           >  1:1250842/1‑68 (MQ=255)                                       ccAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAtgtg                         >  1:3434414/1‑68 (MQ=255)                                          gCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTgaga                      >  1:1560763/1‑68 (MQ=255)                                             gCTGTCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGtaaa                   <  1:5226706/68‑1 (MQ=255)                                              cTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAAc                  <  1:5399667/68‑1 (MQ=255)                                                    aaaCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGAATTAAGTGAGAAAACCGGCAg            <  1:595734/68‑1 (MQ=255)                                                      aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc          <  1:5186665/68‑1 (MQ=255)                                                      aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc          <  1:971053/68‑1 (MQ=255)                                                      aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc          <  1:441396/68‑1 (MQ=255)                                                      aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc          <  1:4238453/68‑1 (MQ=255)                                                      aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc          >  1:3393059/1‑68 (MQ=255)                                                      aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc          <  1:3032603/68‑1 (MQ=255)                                                      aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc          <  1:1337954/68‑1 (MQ=255)                                                        tCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCAt        >  1:4781210/1‑68 (MQ=255)                                                         cACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATg       <  1:410833/68‑1 (MQ=255)                                                           ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc     <  1:4556452/68‑1 (MQ=255)                                                           ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc     <  1:4052077/68‑1 (MQ=255)                                                           ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc     <  1:5323075/68‑1 (MQ=255)                                                           ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc     <  1:603342/68‑1 (MQ=255)                                                           ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc     <  1:2859793/68‑1 (MQ=255)                                                           ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc     <  1:2508043/68‑1 (MQ=255)                                                            cGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACa    <  1:366658/68‑1 (MQ=255)                                                              agaTTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACAtt  <  1:4480827/68‑1 (MQ=255)                                                                  |                                                                GCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATT  >  CP009273/829534‑829662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑