| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 1,660,340 | C→T | P257S (CCC→TCC) | clcB → | H(+)/Cl(‑) exchange transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,660,340 | 0 | C | T | 95.5% | 131.1 / ‑4.4 | 44 | P257S (CCC→TCC) | clcB | H(+)/Cl(‑) exchange transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (18/24); minor base G (1/1); total (19/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGT > CP009273/1660277‑1660403 | ccACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTg < 1:997219/68‑1 (MQ=255) tgttgtTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCaa > 1:2750759/1‑68 (MQ=255) tgttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTg > 1:1396344/1‑68 (MQ=255) ttAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGc < 1:4488599/68‑1 (MQ=255) ttAACGTCAATGAACGCCGGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGGCCTGGCAACTGGc > 1:875787/1‑68 (MQ=255) tAACGTTGATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCa > 1:869292/1‑68 (MQ=255) tAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCa > 1:838055/1‑68 (MQ=255) tAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCa < 1:4738832/68‑1 (MQ=255) tAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCa > 1:3632022/1‑68 (MQ=255) aCGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACt > 1:2344215/1‑68 (MQ=255) aCGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACt > 1:2586228/1‑68 (MQ=255) aCGTTAATGAACGCCTGTCATCGTGGATTTGTGACTCTCAAACTTGCGCCGTCCTGGCAACTGGCACt > 1:2852199/1‑68 (MQ=255) gTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAg > 1:4254424/1‑68 (MQ=255) gTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAg < 1:2996150/68‑1 (MQ=255) ttACTGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAgg < 1:3129118/68‑1 (MQ=255) aaTGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCg < 1:4518225/68‑1 (MQ=255) tGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCggg > 1:103918/1‑68 (MQ=255) tGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCggg > 1:10375/1‑68 (MQ=255) tGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCggg < 1:1211154/68‑1 (MQ=255) tGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCggg < 1:15154/68‑1 (MQ=255) gAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGt > 1:4876601/1‑68 (MQ=255) cGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGa < 1:2931583/68‑1 (MQ=255) cGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGa < 1:5394652/68‑1 (MQ=255) gCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGAt > 1:1298147/1‑68 (MQ=255) gCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGAt > 1:2649059/1‑68 (MQ=255) ccTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATc < 1:4684979/68‑1 (MQ=255) gTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTg < 1:5154272/68‑1 (MQ=255) tCGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTc > 1:1625235/1‑68 (MQ=255) cGTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCt < 1:2275585/68‑1 (MQ=255) gTGGATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTg < 1:181248/68‑1 (MQ=255) ggATTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGCGTTGAACGTGGGACTGca > 1:3026719/1‑67 (MQ=255) aTTTGTGAGTCTCAAACTTGCGCCGTCCTGGCAACAGGCACTAGGCGGGTTGATCGTGGGTCTGCttt > 1:261259/1‑68 (MQ=255) tgtgAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTccc > 1:5597977/1‑68 (MQ=255) gAGTCTCAAACTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGt < 1:4090724/68‑1 (MQ=255) gAGTCTCAAACTTGCGCCGGCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGt < 1:379148/68‑1 (MQ=255) aaaCTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACAcc < 1:5099636/68‑1 (MQ=255) aaaCTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACAcc < 1:4398513/68‑1 (MQ=255) aaaCTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACAcc < 1:4713820/68‑1 (MQ=255) aaaCTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACAcc < 1:1855567/68‑1 (MQ=255) aaCTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCt < 1:4106830/68‑1 (MQ=255) aaCTTGCGCCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCt < 1:337026/68‑1 (MQ=255) gcgcCGTCCTGGCAACTGGGACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgt < 1:2412589/68‑1 (MQ=255) gcgcCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgt < 1:3982153/68‑1 (MQ=255) gcgcCGTCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGGCTGCTTTCCCTGTTCACACCTGCAgt < 1:2877525/68‑1 (MQ=255) gccgtcCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgtgt < 1:1714596/68‑1 (MQ=255) gccgtcCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAgtgt > 1:3618535/1‑68 (MQ=255) | CCACTGTTGTTAACGTTAATGAACGCCTGTCATCGTGGATTTGTGAGTCTCAAACTTGCGCCGCCCTGGCAACTGGCACTAGGCGGGTTGATCGTGGGTCTGCTTTCCCTGTTCACACCTGCAGTGT > CP009273/1660277‑1660403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |