Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,681,289	C→A	D292Y (GAT→TAT)	glrR ←	response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,681,289	0	C	A	100.0%	118.3 / NA	34	D292Y (GAT→TAT)	glrR	response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/13);  total (21/13)

TTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCG  >  CP009273/2681227‑2681352                                                               |                                                                ttctGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTcgc                                                            >  1:3405587/1‑68 (MQ=255) ttctGTGCGCGCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTcgc                                                            <  1:3720141/68‑1 (MQ=255)   ctGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCgg                                                          <  1:770786/68‑1 (MQ=255)   ctGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCgg                                                          <  1:3181122/68‑1 (MQ=255)         gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTcc                                                    >  1:1771824/1‑68 (MQ=255)         gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTcc                                                    >  1:434147/1‑68 (MQ=255)         gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTcc                                                    >  1:5631321/1‑68 (MQ=255)         gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTcc                                                    >  1:2757876/1‑68 (MQ=255)              gCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCcgcg                                               >  1:4752830/1‑68 (MQ=255)              gCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCcgcg                                               >  1:4907535/1‑68 (MQ=255)                cAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCcgcgcg                                             <  1:4568927/68‑1 (MQ=255)                   tGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCa                                          >  1:31200/1‑68 (MQ=255)                       ggAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGc                                      <  1:3096189/68‑1 (MQ=255)                       ggAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGc                                      <  1:4025628/68‑1 (MQ=255)                              tCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGc                               >  1:2797219/1‑68 (MQ=255)                              tCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGc                               <  1:2683929/68‑1 (MQ=255)                                  gCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGAt                           <  1:3184242/68‑1 (MQ=255)                                            ttGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGt                 >  1:3538368/1‑68 (MQ=255)                                              gAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTgg               <  1:4815622/68‑1 (MQ=255)                                                ggCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCCACGCTTTTGGCAGATCTCGGTGAGTGGCa             >  1:5173188/1‑68 (MQ=255)                                                ggCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCa             <  1:1324309/68‑1 (MQ=255)                                                ggCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCa             >  1:2174942/1‑68 (MQ=255)                                                  cGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa           <  1:3820576/68‑1 (MQ=255)                                                  cGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa           <  1:2937868/68‑1 (MQ=255)                                                  cGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa           <  1:249950/68‑1 (MQ=255)                                                          gagaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATcc   >  1:1856752/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGGAGATCACGGTGAGTGGCAGAAATAATCCg  >  1:4947668/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCTCGGTGAGTGGCAGAAATAATCCg  >  1:937187/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg  >  1:4796209/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg  >  1:4603679/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg  >  1:3105421/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg  >  1:55795/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg  >  1:78953/1‑68 (MQ=255)                                                           agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg  >  1:859605/1‑68 (MQ=255)                                                               |                                                                TTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCG  >  CP009273/2681227‑2681352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑