Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,901,251	C→A	G17V (GGT→GTT)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,901,251	0	C	A	100.0%	101.9 / NA	32	G17V (GGT→GTT)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/9);  total (23/9)

ACGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAA  >  CP009273/2901186‑2901314                                                                  |                                                                aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                               >  1:1858513/1‑68 (MQ=255) aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                               >  1:2018710/1‑68 (MQ=255) aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                               >  1:2286947/1‑68 (MQ=255) aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                               >  1:4918607/1‑68 (MQ=255) aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                               <  1:3005230/68‑1 (MQ=255)  cGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAacac                                                              >  1:4409429/1‑68 (MQ=255)    gAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACgg                                                            >  1:904771/1‑68 (MQ=255)    gAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACgg                                                            >  1:4016036/1‑68 (MQ=255)    gAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACgg                                                            >  1:3777871/1‑68 (MQ=255)            gCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCgat                                                    <  1:2762037/68‑1 (MQ=255)                tACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAtt                                                <  1:4509673/68‑1 (MQ=255)                tACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAtt                                                <  1:1220244/68‑1 (MQ=255)                tACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAtt                                                <  1:2310316/68‑1 (MQ=255)                   cgacgaAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCa                                             >  1:516185/1‑68 (MQ=255)                   agacgaAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCa                                             >  1:3685285/2‑68 (MQ=255)                          accaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCga                                      >  1:2817638/1‑68 (MQ=255)                               ccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGAtt                                 <  1:3622758/68‑1 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCTATGATTTTTACGAt                         <  1:3034604/68‑1 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                         >  1:3139507/1‑68 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                         >  1:3439900/1‑68 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                         >  1:3485171/1‑68 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAAAACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                         >  1:718295/1‑68 (MQ=255)                                         tACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAttt                       >  1:220902/1‑68 (MQ=255)                                            ttCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTgg                    >  1:622157/1‑68 (MQ=255)                                            ttCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTgg                    <  1:349600/68‑1 (MQ=255)                                            ttCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTgg                    >  1:4835777/1‑68 (MQ=255)                                            ttCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTgg                    >  1:3140682/1‑68 (MQ=255)                                             tCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGa                   <  1:234880/68‑1 (MQ=255)                                                 cTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACAtt               >  1:2188771/1‑68 (MQ=255)                                                      aCAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGtt          >  1:4342613/1‑68 (MQ=255)                                                       cAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGttt         >  1:373871/1‑68 (MQ=255)                                                             ggTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCa   >  1:5620911/1‑68 (MQ=255)                                                              gTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCaa  >  1:969166/1‑68 (MQ=255)                                                                  |                                                                ACGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAA  >  CP009273/2901186‑2901314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑