| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,439,602 | (T)7→6 | intergenic (‑93/+14) | yhdN ← / ← rplQ | conserved protein YhdN/50S ribosomal subunit protein L17 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,439,596 | 0 | T | . | 84.2% | 20.2 / ‑1.8 | 19 | intergenic (‑87/+20) | yhdN/rplQ | conserved protein YhdN/50S ribosomal subunit protein L17 |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base . (1/15); total (3/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01 | |||||||||||
CACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATC‑AAACTCACC‑TTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑ATAAAAAAACCC‑‑GCCGGGGCGGG‑TTTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCGTTATCACGA‑GTACGGGCG‑AATGCCAGACG‑ACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCG > NC_000913/3439377‑3439851 | cACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATC‑AAACTCACC‑TTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑ATAAAAAAACCC‑‑GCCGGGGCGGG‑‑‑‑TTTTA‑CGTTGCTTCAGATTACTCTGCagcagc < 1:1447659/251‑1 (MQ=255) ttCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGG‑TATC‑AAACTCACC‑TTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGT‑AACCACATAAAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑AT‑AAAAAACCC‑‑GCCGGGGCGGGTTTTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGtt < 1:552858/298‑1 (MQ=255) atcatcCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATC‑AAACTCACC‑TTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TAC‑GGT‑ATAAAA‑‑‑‑‑C‑‑G‑CGGGGCGGG‑‑TTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCG‑TGTCGCCTGCa > 1:1447665/1‑278 (MQ=255) gTTATCAAAACTCACCTTTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGTAAACCACATAAAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑AT‑AAAAAACCC‑‑GCCGGGGCGGG‑‑TTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAAACCAGCTCg < 1:552866/224‑1 (MQ=255) ttCTGCTATATGGCGCTCTGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑ATAAAAAAACCC‑‑GCC‑GGGCGGG‑‑‑‑‑TTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTG‑CGCCTGCACGG‑AAGCCACACTTCAg < 1:1447670/235‑1 (MQ=255) cTGCTATATGGCGCTCTGCCCACTGGTCAAGTAAACCACATAAAATACTCCAGGGGATGAAACAAAAAAGAGTACAGCTATTGTAGATAAAGT‑‑GGGGATAC‑TACGGGT‑AT‑AAAAAACCC‑‑GCCGGGGCGGG‑‑TTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTGAAACGATCAAACCAGCTCGATGTAAAGCCATCGGCGCGTTGTCGCCTGCACGGAAAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGC‑GGCCCAGTTCGTTAAAACAGTTTTGCCACGATCTCGTTATCACGA‑GTACGGGCGAAATGCCAGACG‑ACGATTAGCAACGCTATCAGTCTTGGCaa < 1:552871/378‑1 (MQ=255) cTATATGGCGCTCTGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑ATAAAAAAACCC‑‑GCCGGGGCGG‑‑‑‑‑‑TTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTG‑CGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAAcg < 1:1447671/266‑1 (MQ=255) gcgcTCTGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑AT‑AAAAAACCC‑‑GCCGGGGCGGG‑‑TTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAACACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCGTTATCACGA‑GTACGGGCG‑AATGCCAgac < 1:552872/328‑1 (MQ=255) ctGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑AT‑AAAAAACCC‑‑GCCGGGGCGGG‑‑‑TTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCGAAAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCGTTATCACGA‑g < 1:552873/305‑1 (MQ=255) tCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑AT‑AAAAAACCC‑‑GCCGGGGCGGG‑‑‑TTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAAACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGTAAACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAG‑TTTGCCACGATCTCGTTATCACGA‑‑TACGGGCG‑AATGCCAGACG‑ACGATTAGCAACGCTATCAGTCtt < 1:552874/336‑1 (MQ=255) gATG‑AAC‑‑AAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑ATAAAAAAACCC‑‑GCCGGGGCGGG‑‑TTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCG‑TGTCGCCTGCACGG‑‑AGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCGTTATCACGA‑GTACGGGCG‑AATGCCAGACGAACGAtttagca < 1:552875/296‑6 (MQ=255) cTATTGTAGAT‑AAGTGAGGGGATACGTACGGGTAATCAAAAAACCCCGGCCGGGGCGGG‑‑‑‑TTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCGTTATCACGA‑GTACGGGCG‑AATGCCAGACG‑ACGATTAGc < 1:1447675/280‑1 (MQ=255) gTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑ATAAAAAAACCC‑‑GCCGGGGCGGG‑tttttta > 1:1447678/1‑54 (MQ=255) aaGT‑‑GGGGATAC‑TACGGGT‑AATAAAAAACCC‑‑GGCCGGGCGGG‑‑‑‑TTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAg < 1:1447677/152‑1 (MQ=255) ggtaatcaaaaaacCCG‑‑GCCGGGGCGGG‑‑‑‑TTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGAtct < 1:1447679/202‑1 (MQ=255) taaaaaaCCC‑‑GCCGGGGCGGG‑‑‑‑TTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCG‑TGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCGAAAACGCGGGCCCAGTTCGTT‑aa < 1:552878/183‑1 (MQ=255) aaaaaCCC‑‑GCCGGGGCGGG‑TTTTTTTATCGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCGTTATCACGA‑GTACGGGCG‑AATGCCAGAcg‑acga > 1:1447682/1‑239 (MQ=255) aaCCC‑‑GCCGGGGCGGG‑‑‑TTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAAcg < 1:552879/163‑1 (MQ=255) ccc‑‑GCCGGGGCGGG‑TTTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACG‑TCTCGTTATCACGATGTACGGGCG‑AATGCC‑GACG‑ACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCg > 1:1447684/1‑269 (MQ=255) cccggccgGGGCGGG‑‑‑‑TTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCg < 1:1447681/194‑1 (MQ=255) | CACGTAATTCCGGTGGCACGTGAGAATCATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATC‑AAACTCACC‑TTTCGCTTGCGCTTCTGCTATATGGCGCTCTGCCCACTGGTCAAGT‑AACCACAT‑AAATACTCCAGGGGATG‑AAC‑AAAAAGAGTACAGCTATTGTAGAT‑AAGT‑‑GGGGATAC‑TACGGGT‑ATAAAAAAACCC‑‑GCCGGGGCGGG‑TTTTTTTA‑CGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATC‑AACCAGCTCGATGT‑AAGCCATCGGCGCGTTGTCGCCTGCACGG‑AAGCCACACTTCAGAATACGAGTGT‑AACCACCGGCACGGCTCGCG‑AAACGCGGGCCCAGTTCGTT‑AAACAGTTTTGCCACGATCTCGTTATCACGA‑GTACGGGCG‑AATGCCAGACG‑ACGATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCG > NC_000913/3439377‑3439851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |