| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 697,144 | (T)8→9 | intergenic (‑11/+369) | metT ← / ← asnB | tRNA‑Met/asparagine synthetase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 697,136 | 1 | . | T | 76.9% | 28.2 / ‑1.5 | 13 | intergenic (‑3/+377) | metT/asnB | tRNA‑Met/asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (8/2); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
AAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGG‑TGGCT‑ACGACGGGATTCGAACCTGTGACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATC‑‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTC‑TTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTG‑TTTTTTCCCACCCTACAGCCATTCTTTTG‑TCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGC > NC_000913/696908‑697386 | aaaCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGC‑TTCTGGTG‑ATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGC‑GCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGG‑TGGCT‑ACGACGGGATTCGAACCTGTGACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATCT‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGgc‑gc < 1:829344/264‑1 (MQ=255) ttCGCCACTCCCGC‑AAAAAACATGGCTGGCTAACGACGGGATTCGAACCTGTGACCCCATCACTTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCTCTT‑‑‑‑‑‑‑‑‑TCTTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATACG‑AGCCTTGCAGCGTCAACCTC‑TTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTc < 1:91128/247‑1 (MQ=37) gggATTCGAACCTGTGACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATCT‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑t > 1:829376/1‑82 (MQ=255) cGAACCTGTGACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATCTT‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTCTTTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGgc > 1:829378/1‑191 (MQ=255) cGAACCTGTGACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATCT‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GCGA‑TTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTC‑tt > 1:829379/1‑135 (MQ=255) gAACCTGTGACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATCT‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGT‑‑ATCCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATA‑GTAGCCTTGCAGCGTCAACCTCTTTTTTCAAGGAAAATTGCTCg > 1:829381/1‑154 (MQ=25) tgACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATCTTTTTTTTTT‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTC‑TTTTTCAAGGaa < 1:829375/144‑1 (MQ=25) aTGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATCTTT‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GCGATTTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTC‑TTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTGTTTTTTTCCCACCCTACAGCCATTCTTTTGTTCATACa > 1:91130/1‑241 (MQ=255) tGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATC‑‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCtt < 1:829382/58‑1 (MQ=255) aCCAACTGAGCTACGTAGCC‑ATCT‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTggc > 1:829385/1‑53 (MQ=255) aCCAACTGAGCTACGTAGCC‑ATCT‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTC‑TTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTG‑‑TTTTTCCCACCCTACAGCCATTCTTTTG‑TCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGc > 1:829384/1‑273 (MQ=255) aCCAACTGAGCTACGTAGCC‑ATC‑‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGATTTACCTTATCGGCGTTGCGGGGCGGC‑A‑TTa > 1:829386/1‑67 (MQ=255) gCC‑ATCT‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTC‑TTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTG‑‑TTTTTCCCACCCTACAGCCATTCTTTTG‑TCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATAc > 1:829390/1‑252 (MQ=255) | AAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTGCGGGAGGCGAGACTTGAACTCGCACACCTTGCGGCGCCAGAACCTAAATCTGGTGCGTCTACCAATTTCGCCACTCCCGCAAAAAAAGATGG‑TGGCT‑ACGACGGGATTCGAACCTGTGACCCCATCA‑TTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCC‑ATC‑‑‑‑‑‑‑‑‑T‑TTTTTTTCGCGA‑‑TACCTTATCGGCGTTGCGGGGC‑GC‑A‑TTATGCGTATA‑G‑AGCCTTGCAGCGTCAACCTC‑TTTTTCAAGGAAAATTGCTCGAAAGTGACTGTTTGGTTAGGTTGCGAACAGCGTGGCGCTATATTCGTCAATTATTGTTTACTTTGTG‑TTTTTTCCCACCCTACAGCCATTCTTTTG‑TCATACAGGATGAAATTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGC > NC_000913/696908‑697386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |