Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	926,186	+T	intergenic (‑215/+39)	serW ← / ← infA	tRNA‑Ser/translation initiation factor IF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	926,182	1	.	T	75.0%	78.3 / 9.3	40	intergenic (‑211/+43)	serW/infA	tRNA‑Ser/translation initiation factor IF‑1
Reads supporting (aligned to +/- strand):  ref base . (9/1);  new base T (1/29);  total (10/30)
Fisher's exact test for biased strand distribution p-value = 3.55e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-06

GATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTC‑TTC‑TTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAAC‑GGGGCGCTACTATAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTC‑TTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGA‑TTTGCGCGCTAAATCGCAACAAACAGGTTCGGCACATTACTCCGAAAACACACGGCTAAGCCG  >  NC_000913/925832‑926579                                                                                                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                                                                                                                                                 gaTAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTCTTTCTTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAAC‑GGGGCGCTACTATAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCCAAAAGTACTTCATACATATCAC‑AAA‑ATAAAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGtt                                                                                                                                                                                                                                                                                                                                                                                                                       <  1:114626/368‑1 (MQ=255)                                                                                           gCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGGCGCTACTATAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTa                                                                                                                                                                                                                                                                                                                                                                          <  1:861189/317‑1 (MQ=255)                                                                                             cGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAAC‑GGGGCGCTACTATAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGc                                                                                                                                                                                                                                                                                                                                                                               <  1:861188/309‑1 (MQ=255)                                                                                                                    cTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAAC‑GGGGCGCTACTATAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAc                                                                                                                                                                                                                                                                                                                                                                  <  1:861192/299‑1 (MQ=255)                                                                                                                                              ccAAATTG‑TTTGCTACCAAACCTCATGGGTGGCAAC‑GGGGCGCTACTATAGGGAGTT‑GGAGT‑AAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTG‑AAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAAc                                                                                                                                                                                                                                                                                                                     <  1:114638/310‑1 (MQ=255)                                                                                                                                                                    cTCATGGGTGGCAAC‑GGGGCGCTACTATAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAATTTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGACTTTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCCTTTTGCTCAGGTTCGTGAC‑GGGGTCAG‑TTCAACAG‑CAC‑TTTCGTCG‑CCCGTCAGGATGCGGATGTAGTTTTTTGCGCATTTTTACCGgag                                                                                                                                                                                                                                                         <  1:114641/354‑1 (MQ=255)                                                                                                                                                                                             ctaTAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑‑AA‑AT‑‑AAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAACAAATCAGCGACTACGGAAGAC‑AATGCGGCCTTTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCACTTTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑ttttgc                                                                                                                                                                                                                                                                         >  1:861203/1‑305 (MQ=255)                                                                                                                                                                                              tataGGGAGTTAGGAGTAAAACGGTCAAGAATGAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGc                                                                                                                                                                                                                                                                                                                                                                               <  1:114645/216‑1 (MQ=255)                                                                                                                                                                                                taGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCCTTTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTgc                                                                                                                                                                                                                                                                        >  1:861204/1‑306 (MQ=255)                                                                                                                                                                                                  gggAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑tt                                                                                                                                                                                                                                                                                                                                                     <  1:861198/234‑1 (MQ=255)                                                                                                                                                                                                   ggAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑aa                                                                                                                                                                                                                                                                                                                                                               <  1:861200/224‑1 (MQ=255)                                                                                                                                                                                                    gAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑aa                                                                                                                                                                                                                                                                                                                                                               <  1:861199/223‑1 (MQ=255)                                                                                                                                                                                                               aaaaCGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACC‑tttt                                                                                                                                                                                                                                                                                                                                                                                                                              <  1:861201/154‑1 (MQ=255)                                                                                                                                                                                                                                  ttttAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTCTTTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGgaga                                                                                                                                                                                                                                                        <  1:861206/290‑1 (MQ=255)                                                                                                                                                                                                                                           tAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑‑AAAAGG‑CCGG‑T‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCGCCCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGTGAC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑‑TTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAAc                                                                                                                                                                                                                      <  1:114648/311‑1 (MQ=255)                                                                                                                                                                                                                                                attGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGACAAAGTTACGGAC‑TTATACTTCCAAAAGTACTTCATACATATCACAAAA‑ATAAAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTCTTTTCTCTTCGCCCCATCAGGCGGTAAAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCCTTTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTc                                                                                                                                                                                                                                                                                                                        <  1:114649/224‑1 (MQ=255)                                                                                                                                                                                                                                                  tGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑‑AA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCCTTTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑‑TT‑GTCG‑CCCGTCAGGATGCGGATG‑AG‑‑TTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTC‑TTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAAtt                                                                                                             <  1:114650/406‑1 (MQ=255)                                                                                                                                                                                                                                                              cTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAATAA‑AAAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATg                                                                                                                                                                                                                                                                                         >  1:114655/1‑230 (MQ=255)                                                                                                                                                                                                                                                                        g‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑tt                                                                                                                                                                                                                                                                             <  1:861208/231‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGAtgtg‑tg                                                                                                                                                                                                                                                 <  1:861210/249‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tataTCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAg                                                                                                                                                                                                                                                                                             <  1:114654/207‑1 (MQ=255)                                                                                                                                                                                                                                                                                          cATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑‑TTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGaa                                                                                                                                                                                                           <  1:861211/276‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aTCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGt                                                                                                                                                                                                                                             <  1:861212/243‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gagaC‑AAGTTACGGAC‑TTATACTTCC‑‑AAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAACAAATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑‑TTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTAt                                                                                                                                                                                                   <  1:861213/280‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         aCGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAAc                                                                                                                                                                                                                      >  1:861219/1‑251 (MQ=255)                                                                                                                                                                                                                                                                                                              c‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑‑AA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑‑AAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑‑GGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATG‑AG‑‑‑TTTGCGCA‑‑TTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑‑TTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCa                                                                                                                                                                <  1:114657/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                   taCTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGgaga                                                                                                                                                                                                                                                        <  1:861215/211‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                        cc‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTTAAAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAc                                                                                                                                                                                                                                                                                                                                                                  <  1:861217/112‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                        cc‑AAAGTACTTCATACATATCAC‑‑AA‑AT‑AAAAAGGACCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCCTTTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAGTTTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCt                                                                                                                                                                                                                         >  1:114659/1‑236 (MQ=255)                                                                                                                                                                                                                                                                                                                                aCTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑‑TTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATAtt                                                                                                                                                           <  1:861218/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                                    catacataTCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑ccc                                                                                                                                                                                                                                                                                                                                                                                                       >  1:861223/1‑64 (MQ=255)                                                                                                                                                                                                                                                                                                                                    catacataTCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCA‑GCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCACTTTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCGTTTTTCTAACTCTACGCGGAACATGGTATTAGGCAAC‑TTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTC‑TTTGGCCATc                                                                                                                                          >  1:861224/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                                                    catacataTCAC‑‑AA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTaa                                                                                                                                                                                                                       <  1:861220/225‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                                    catacataTCAC‑‑AA‑AT‑AAAAAGG‑CCGGT‑‑‑AACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCACTTTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑‑TTTTGCGCA‑TTTTACCGGAGATGTGTTGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTCTTTTGGCCATCTAATCCTCT‑GGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGATTTTGCGCGCTAAATCGCAACAAACAGGTTCGGCACATTACTCCGAAAACACACGGCTAAGCCg  <  1:114658/436‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                                           atCAC‑AAA‑TA‑AAAGAAG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGACGAATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACC‑GAGAtgtg                                                                                                                                                                                                                                                    >  1:114661/1‑191 (MQ=255)                                                                                                                                                                                                                                                                                                                                            tCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCt                                                                                                                                                                                                                         <  1:861221/216‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                                              tcacaa‑aT‑AAAAAGG‑CCGGTTAAAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACT‑CGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAAC‑TGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTC‑TTTGGCCATCTAATCCTCTGGGGTATCACT‑CCGTAATTTGAACCgg                                                                                                     >  1:114662/5‑327 (MQ=255)                                                                                                                                                                                                                                                                                                                                               c‑aAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGt                                                                                                                                                                             <  1:861222/256‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                                                                    ttaaaaCCGACC‑TTTTACTCGTTC‑TTTCTCTTCGCCCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑ACGGGGGTCAGTTTCAACAGTCAC‑TTT‑GTCGCCCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGttt                                                                                                                                                                                       >  1:114663/4‑234 (MQ=255)                                                                                                                                                                                                                                                                                                                                                                     t‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTC‑TTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAAttt                                                                                                            <  1:861225/303‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                                                                     t‑AAACCGACCTTTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑‑TTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTc                                                                                                                                                                                      <  1:861226/229‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                                                                                                                                                 GATAAAATGCGAAAAAAAAGCTCGCACTTTCGTACGAGCTC‑TTC‑TTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAAC‑GGGGCGCTACTATAGGGAGTT‑GGAGTAAAACGGTCAAGAA‑GAA‑TTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAG‑TTGTGCAGTATATCTACATCGAGAC‑AAGTTACGGAC‑TTATACTTCC‑AAAGTACTTCATACATATCAC‑AAA‑AT‑AAAAAGG‑CCGGTT‑AAACCGACC‑TTTTACTCGTTC‑TTTCTCTTCG‑CCCATCAGGCGGT‑AAAAC‑AATCAGCGACTACGGAAGAC‑AATGCGGCC‑TTTGCTCAGG‑TCGT‑AC‑GGGGTCAG‑TTCAACAGTCAC‑TTT‑GTCG‑CCCGTCAGGATGCGGATGTAG‑TTTTTGCGCA‑TTTTACCGGAGATGTG‑TGCAGTAACCACGTGACCG‑TTTTCTAACTCTACGCGGAACATGGTATTAGGCAACGTTTCAAGAACGGTACCTTGCATTTCAATATTGTCTTC‑TTTGGCCATCTAATCCTCTGGGGTATCACTACCGTAATTTGAACCGGCAAGATAATGCCGAAGTTCTGTAAATAAGTAAAGA‑TTTGCGCGCTAAATCGCAACAAACAGGTTCGGCACATTACTCCGAAAACACACGGCTAAGCCG  >  NC_000913/925832‑926579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑