Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913618,0840T.61.1% ‑1.8 / 10.1 18intergenic (+46/‑170)entF/fepEapo‑serine activating enzyme/polysaccharide co‑polymerase family protein FepE
Reads supporting (aligned to +/- strand):  ref base T (6/1);  new base . (3/8);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 4.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACG‑TTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGGG‑TTTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGT  >  NC_000913/617806‑618314
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
aCGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGGG‑t                                                                                                                                                                                                                                               >  1:818187/1‑280 (MQ=255)
                    ctgACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAA‑CCAT‑AATTAC‑AGAAAATAATTATGGG‑t                                                                                                                                                                                                                                               >  1:818190/1‑259 (MQ=255)
                                                                                    ttCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACGTTTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAA‑CCAT‑AATTAC‑AGAAAATAATTATGGG‑ttttt                                                                                                                                                                                                                                           >  1:818195/1‑199 (MQ=255)
                                                                                              tATGAGTCCCG‑ACGCGCCTGGTCG‑CGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGG‑CG‑TTTGAAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGG‑‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑a                                                                                                                                                       <  1:818193/262‑1 (MQ=255)
                                                                                                   gTCCCG‑ACGCGCCTGGTCG‑CGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACG‑TTTGAAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGG‑‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGaa                                                                                                                                                                                                          <  1:818194/209‑1 (MQ=255)
                                                                                                             cgcCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACG‑TTTGAAAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGGG‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTACAAAACTTCAGCTTCgg                                                                                                                                         <  1:818196/266‑1 (MQ=255)
                                                                                                                                          atatCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACG‑TTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGG‑‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCa                                                                                                                                                                            <  1:818198/200‑1 (MQ=255)
                                                                                                                                                          ttGTGCGCATGTGGATATTATCTCTCCAGGGACG‑TTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTACAAGAAAATAATTATGG‑‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGaa                                                                                                                                                                                                          <  1:818199/156‑1 (MQ=255)
                                                                                                                                                                            tatCTCTCCAGGGACG‑TTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAA‑CCAT‑AATTAC‑AGAAAATAATTATGGG‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCa                                                              >  1:818203/1‑275 (MQ=255)
                                                                                                                                                                             atCTCTCCAGGGACGTTTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAAC‑GGTAAATTAAATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGGG‑TTTTTTA‑TTTGTTTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCctct       >  1:82730/1‑333 (MQ=255)
                                                                                                                                                                                              ttgaaaaaaaTTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAA‑CCAT‑AATTAC‑AGAAAATAATTATGGG‑TTTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTta                                                                     >  1:818204/5‑253 (MQ=255)
                                                                                                                                                                                                                      cgcgCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGGG‑TTTTTTA‑TTTG‑TTTGTA‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTc                    >  1:818206/1‑280 (MQ=255)
                                                                                                                                                                                                                         gcAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AG‑AAATAATTATGG‑‑‑TTTTTA‑TTTG‑TTTG‑A‑‑TTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTgg                                                                                                      <  1:818205/190‑1 (MQ=255)
                                                                                                                                                                                                                                         aaaTT‑AATATTATTTATAAAACCCAT‑AATTAC‑AGAAAATAATTATGGG‑‑TTTTTA‑TTTGTTTTG‑ATTTTATTAGGTTTGATGAATATTTCTCTTAAAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCTACTTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGt  >  1:82735/1‑282 (MQ=255)
                                                                                                                                                                                                                                         aaaTT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGGT‑TTTATTG‑TTT‑‑TGT‑‑A‑TTTA‑TTAGTTGTATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCAtttt                                                                            >  1:82736/1‑202 (MQ=255)
                                                                                                                                                                                                                                                   ttatttat‑‑AACCCAT‑AATTACAAGAAATTAAGTATTGG‑‑TTTTTA‑‑TTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGt                                 <  1:82734/236‑1 (MQ=255)
                                                                                                                                                                                                                                                     atttat‑AAACCCATAAATTAC‑AGAAAATAATTATGGG‑TTTTTTA‑TTTGTTTTG‑A‑TTTA‑TAGGttttga                                                                                                                                                                                                             >  1:82737/1‑65 (MQ=255)
                                                                                                                                                                                                                                                            aaaCCCAT‑AATTAC‑AGAAAATAATTATGG‑‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTccccc                                                                                           <  1:818207/170‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCCCAT‑AATTACAAGAAAATAATTATGGT‑TTTTTAT‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAat                                                                                                                                                                                                         <  1:818208/63‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aTTAC‑AGAAAATAATTATGGG‑‑TTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCAt                                                             >  1:818209/1‑192 (MQ=255)
                                                                                                                                                                                                                                                                                       aTGGGTTTTTTTATTTTGTTTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGt                                                                                                                      >  1:818211/1‑123 (MQ=255)
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
ACGCTGATGCTGTGCGCCTGCTGACGACTGCTCATAGCGTACCGTTTGACGGTAAAGCGACGCTGTTTGTTGCTGAACGCACACTTCAGGAAGGTATGAGTCCCGAACGCGCCTGGTCGCCGTGGATAGCGGAGCTGGATATCTATCGTCAGGATTGTGCGCATGTGGATATTATCTCTCCAGGGACG‑TTTG‑AAAAAATTGGGCCGATTATTCGCGCAACGCTAAACAGGTAAATT‑AATATTATTTAT‑AAACCCAT‑AATTAC‑AGAAAATAATTATGGG‑TTTTTTA‑TTTG‑TTTG‑A‑TTTA‑TAGGTTTGATGAATATTTCTCTT‑AAATAGAGTGAATGTTGCATTCAGTGGAAGGAATTTAC‑AAACTTCAGCTTCGGATAATTATCACCAACTGGTAAAGTGAGCGCCGTGGAGATTTCCCCCTATTGGACTCATTTTCAGGTTATACCCCATGTCATCACTGAATATTAAACAGGGAAGTGACGCTCATTTTCCCGATTATCCTCTGGCGT  >  NC_000913/617806‑618314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑