Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,584,928	0	T	.	60.8%	32.2 / 28.9	51	coding (1559/2280 nt)	ydeP	putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base T (10/10);  new base . (13/18);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGCCTTACCTGACGGCGT‑CATCCAGCGCC‑TTTCAGCA‑GCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCC‑AGACGAGCCAGAAACTCTGCAGACGGTTTCTCGGTGATACCGACGGTTCGGTCGCCCTGTACATTAGAGTGTCCACGTAGTGGGCA  >  NC_000913/1584621‑1585289                                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                                                                                                     aGCCTTACCTGACGGCGTCCATCCAGCGCCTTTTCAGCAGGCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTGTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGAt                                                                                                                                                                                                                                                                                                              <  1:320552/383‑1 (MQ=255)                           cgcC‑TTTCAGCA‑GCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑‑TTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGc                                                                                                                                                                                                                                                                                                                               >  1:320559/1‑334 (MQ=255)                                                ttATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCt                                                                                                                                                                                                                                                                                               <  1:320558/348‑1 (MQ=255)                                                     aGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑G‑CCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAAtct                                                                                                                                                                                                                                                                                                                                                                   >  1:1137456/1‑275 (MQ=255)                                                                       tGACGGATGCGCTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGaa                                                                                                                                                                                                                                                                                                                                           <  1:1137454/281‑1 (MQ=255)                                                                                 gcTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑Gttttt                                                                                                                                                                                                                                                                                                                                                                                  >  1:1137461/1‑234 (MQ=255)                                                                                                  aaCTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTg                                                                                                                                                                                                                                                                                                                                                                               <  1:1137460/220‑1 (MQ=255)                                                                                                                    tCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑Tc                                                                                                                                                                                                                                                                                                                                                                        <  1:1137464/207‑1 (MQ=255)                                                                                                                            aTTGCGAAATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCTGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑‑TTTACA‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTGTCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGa                                                                                                                                                                                                                                >  1:320566/1‑332 (MQ=255)                                                                                                                              tGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTT‑CCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGagag                                                                                                                                                                                                                                                                                                         >  1:1137467/1‑258 (MQ=255)                                                                                                                                           cATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCG‑TTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGcgctcgct                                                                                                                                                                                                                                       >  1:320570/1‑310 (MQ=255)                                                                                                                                                tCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑G‑CCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑‑TTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑a                                                                                                                                                                                                                                                                                                                    >  1:320573/1‑229 (MQ=255)                                                                                                                                                    ccaccaGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑‑TTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTG‑CCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACa                                                                                                                                                                <  1:320565/366‑1 (MQ=255)                                                                                                                                                      accaGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCACCGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑‑‑TTTTGCATG‑TCAATCTCGCTACGTccc                                                                                                                                                                                                                                                                                                                                                        <  1:320567/189‑1 (MQ=255)                                                                                                                                                         aGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACgg                                                                                                                                                                                                                                                      <  1:1137468/282‑1 (MQ=255)                                                                                                                                                             aCTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACg                                                                                                                                                                                                                                                       >  1:320575/1‑279 (MQ=255)                                                                                                                                                             aCTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCa                                                                                                                                                                                                                                                                          >  1:1137476/1‑259 (MQ=255)                                                                                                                                                                cccAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGAt                                                                                                                                                                                                                                                                                                              <  1:1137469/221‑1 (MQ=255)                                                                                                                                                                    ggCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAgcg                                                                                                                                                                                                                                             <  1:1137471/280‑1 (MQ=255)                                                                                                                                                                    ggCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACgtgt                                                                                                                                                                                                                                                                                 <  1:1137470/245‑1 (MQ=255)                                                                                                                                                                      cTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑‑TTTTTGCATG‑TCAA‑CTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTTCCCCGATCTGGCATTGCCCAGCGCAAAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATGTCCAGGTGCATGa                                                                                                                    <  1:320571/394‑1 (MQ=255)                                                                                                                                                                        aCCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGaa                                                                                                                                                                                                                                                                                                                                           <  1:1137473/185‑1 (MQ=255)                                                                                                                                                                         ccACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑‑TTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGtt                                                                                                                                                                                                                                                           <  1:1137474/260‑1 (MQ=255)                                                                                                                                                                          cACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTGTTTTTTTGCATG‑TCAATCTCGCTACGTccc                                                                                                                                                                                                                                                                                                                                                        <  1:320574/174‑1 (MQ=255)                                                                                                                                                                           aCGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACgt                                                                                                                                                                                                                                                                                   >  1:320576/1‑238 (MQ=255)                                                                                                                                                                                ctGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGC‑T‑CCCGATCTGGCATTG‑CCAGCGC‑AAA‑‑TGTCCCCACTGCAGATCAATGCt                                                                                                                                                                                 >  1:320577/1‑321 (MQ=255)                                                                                                                                                                                ctGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTAcc                                                                                                                                                                                                                                                                              >  1:1137479/1‑237 (MQ=255)                                                                                                                                                                                ctGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAgcg                                                                                                                                                                                                                                             >  1:1137480/1‑266 (MQ=255)                                                                                                                                                                                                     gATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑c                                                                                                                                                                                                                                    <  1:1137478/254‑1 (MQ=255)                                                                                                                                                                                                                           ctctGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATGTTCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACgt                                                                                                                                                                                                                                                                                   >  1:1137483/1‑190 (MQ=255)                                                                                                                                                                                                                           ctctGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACgt                                                                                                                                                                                                                                                                                   >  1:1137484/1‑189 (MQ=255)                                                                                                                                                                                                                           ctctGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACgt                                                                                                                                                                                                                                                                                   >  1:1137482/1‑189 (MQ=255)                                                                                                                                                                                                                            ctgattttCAGCATTACACCGGCGGG‑TTTTAACAACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTCAAACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCTAAATTGCGTTAACGG‑TAACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCAC‑TGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATg                                                                                                                                 >  1:320582/6‑332 (MQ=255)                                                                                                                                                                                                                                  ttCAGCATTACACCGGCGGG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTA‑CGCCTGCGCACC‑‑‑‑TGTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGC‑TAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTAc                                                                                                                                                                 <  1:320580/287‑1 (MQ=255)                                                                                                                                                                                                                                  ttCAGCATTACA‑CGGCGGGTTTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAA‑GGTTACCGCCTGCGCACC‑‑G‑‑TTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGC‑TAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGCAAAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATAttgcttg                                                                                                                                                       <  1:320579/298‑1 (MQ=255)                                                                                                                                                                                                                                   tCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACa                                                                                                                                                                                                                                                                                                                 >  1:1137487/1‑151 (MQ=255)                                                                                                                                                                                                                                               cggcggG‑TTTTAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑‑TGTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACgg                                                                                                                                                                                                                                                      <  1:320581/197‑1 (MQ=255)                                                                                                                                                                                                                                                gcgggg‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCC‑AATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGC‑T‑CCCGATCTGGCATTG‑CCAGCGC‑‑AAATTGCCCCCACTGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCC‑AGACGAGCCAGAAACTCTGCAGACGGTTTCTCGGTGATACCGACGGt                                         >  1:320586/4‑392 (MQ=255)                                                                                                                                                                                                                                                       ttttAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTGCCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑aaa                                                                                                                                                                                                          <  1:1137485/231‑1 (MQ=255)                                                                                                                                                                                                                                                       ttttAAC‑ACG‑CACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTA‑GTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATAttgcttg                                                                                                                                                       <  1:1137486/279‑1 (MQ=255)                                                                                                                                                                                                                                                             c‑acgccacgcGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑‑‑TTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑aaa                                                                                                                                                                                                          <  1:320583/222‑1 (MQ=255)                                                                                                                                                                                                                                                               acgccacgcGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTGTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGC‑TAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATGTCCAGGt                                                                                                                          <  1:320584/302‑1 (MQ=255)                                                                                                                                                                                                                                                                   cacgcGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGc                                                                                                                                             <  1:1137488/279‑1 (MQ=255)                                                                                                                                                                                                                                                                    acgcGAGGCATGAATCATCGACA‑TGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AAtt                                                                                                                                                                                                                                                                >  1:1137493/1‑168 (MQ=255)                                                                                                                                                                                                                                                                      gcgAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCA‑CGC‑AAAATTGCCCCCCATGCAGATc                                                                                                                                                                                       <  1:1137491/234‑1 (MQ=255)                                                                                                                                                                                                                                                                      gcgAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑‑TTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATg                                                                                                                                 <  1:1137490/287‑1 (MQ=255)                                                                                                                                                                                                                                                                                aaTCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAA‑TGCCCCCACTGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCC‑AGACGAGCCAGaa                                                                           >  1:320587/1‑331 (MQ=255)                                                                                                                                                                                                                                                                                     tcGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑‑TTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAAGATA‑GGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATc                                                                                                                                                                                       <  1:1137492/218‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gAATCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑‑AAATTGCCCCAC‑TGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTGCCATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCCGAGACGAGCCAGAAACTCTGCAGACGGTTTCTCGGTGATACCGACGGTTCGGTCG‑CCTGTACATTAGAGTGTCCACGTAGTGGGCa  >  1:320589/1‑390 (MQ=255)                                                                                                                                                                                                                                                                                               aTCCTCAACGGTTACCGCCTGCGCACC‑‑G‑TTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑‑ATTGCGTTAACGG‑T‑ACAGcgctcgc                                                                                                                                                                                                                                        >  1:1137494/1‑163 (MQ=255)                                                                                                                                                                                                                                                                                                     aaCGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATGTTCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGCTTATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCC‑AATCT‑AATTGCGTTAACGGTT‑ACAgcgc                                                                                                                                                                                                                                            >  1:320590/1‑157 (MQ=255)                                                                                                                                                                                                                                                                                                          ttACCGCCTGCGCACC‑‑‑TGTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGC‑TAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑aa                                                                                                                                                                                                           <  1:320588/178‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                             tttttttGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCt                                                                                              >  1:1137498/1‑271 (MQ=255)                                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                                                                                                     AGCCTTACCTGACGGCGT‑CATCCAGCGCC‑TTTCAGCA‑GCTGCATTTATCAGGTGAAAACCACCGGGATGACGGATGCGCTGGTTATAGTCGGCGAACTCTGGCAGCACAGCTTCAATGTCATTGCG‑AATGCGATCATAATCTTCCACCAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGC‑GACCACTGCACACTCTGATTTCAGCATTACACCGGCGGG‑TTTTAAC‑ACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACC‑‑GTTTTTTTGCATG‑TCAATCTCGCTACGTCCCAGGACCGGCAGAATATAGC‑TATGCCGTGCGGTC‑AACAGATGAGAGCGGTTAAGCTTAGTGGCTACGTGTACCGCCAAATCT‑AATTGCGTTAACGG‑T‑ACAGCGCTCGCTT‑CCCGATCTGGCATTG‑CCAGCGC‑AAAATTGCCCCCCATGCAGATCAATGCTCGAGCCTGCCCCGTACATATTGCTTG‑CATGCTGGCAATTGCAGCATGTCCAGGTGCATGAGGTGGGGTGAAGCCATAGCGCTCACCC‑AGACGAGCCAGAAACTCTGCAGACGGTTTCTCGGTGATACCGACGGTTCGGTCGCCCTGTACATTAGAGTGTCCACGTAGTGGGCA  >  NC_000913/1584621‑1585289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑