Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT66.7% 2.9 / 13.7 20intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base T (5/7);  total (8/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTG‑ATGCGACGC‑TTAACGCG‑TCTTA‑TCAGGCCTAC‑GCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTGCCGCGTCTT‑ATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGG  >  NC_000913/4295903‑4296142
                                                                                                                                                                        |                                                                                    
aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTG‑ATGCGACGC‑TTAACGCG‑TCTTA‑TCAGGCCTAC‑GCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGATGCGACGC‑t                                                                      <  1:1569152/173‑1 (MQ=14)
          tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGCATGCGACGC‑TTAACGCGTTCTTACTCAGGCCTACGGCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑‑TTACGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTGCCGCGTCTT‑ATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt                 <  1:1569155/218‑1 (MQ=11)
                                   ggACATCAACGCCTG‑ATGCGACGC‑TTAACGCG‑TCTTA‑TCAGGCCTAC‑GCCAGACAGCGCTAATAGCCTGA‑TTTAGGCGTGATTTT‑GTAGGT‑C‑GATAAGGCG‑TTTACGCCGCATCCGAC‑TCAATGCCTGATg                                                                              >  1:643940/2‑131 (MQ=11)
                                                tG‑ATGCGACGC‑TTAACGCG‑TCTTA‑TCAGGCCTAC‑GCCAGACAGCGC‑AATAGCCTGA‑‑TTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCtt                                                           >  1:643955/1‑135 (MQ=255)
                                                gctaTGCGACGCTTTAACGCG‑TC‑TA‑TCAGGCCTAC‑GCCAGACAGCGC‑AATAGCCTGATTTTA‑GCGTGATTTTGGTAGGTCCGGATAAGGCG‑TTTACGCCGCATCCGAC‑TCAATGCCTGATGCGACGCTTTGCCGCGTCTT‑ATccag                                                    >  1:1569180/4‑143 (MQ=11)
                                                   aTGCGACGC‑TTAACGCG‑TCTTA‑TCAGGCCTAC‑GCCAGACAGCGCTAATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCGTTTTACGCCGCATCCGACATCAA‑GCCTGATGCGAc                                                                          >  1:643941/1‑121 (MQ=11)
                                                                         tA‑TCAGGCCTAC‑GCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCGTTTTACGCCGCATCCGACATCAATGCCTGATGCGACGC‑TTg                                                                    >  1:643956/1‑106 (MQ=255)
                                                                                 ccTAC‑GCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGATGCGACGC‑tt                                                                     <  1:1569164/97‑1 (MQ=18)
                                                                                    aC‑GCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAA‑GCG‑TTACAGCCGCAT‑CGACATCAA‑GCCTGATGCGACGC‑TTGCCGCGTCTT‑a                                                         <  1:1569165/102‑1 (MQ=11)
                                                                                     c‑GCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGATGc                                                                             <  1:643943/86‑1 (MQ=18)
                                                                                     c‑GCCAGACAGCGC‑AATAGCCTGA‑‑TTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAACGCCTGATGCGACGC‑‑TGCCGCGTCTT‑ATCAGGcc                                                  <  1:643948/109‑1 (MQ=255)
                                                                                          aGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAACGCCTGATGCGACGC‑tt                                                                     <  1:643945/89‑1 (MQ=33)
                                                                                            aCAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTT‑At                                                        <  1:1569166/99‑1 (MQ=255)
                                                                                              aGCGC‑AATAGCCTGA‑TTTA‑GCGTGA‑TTT‑GTAGGT‑CGTATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGAt                                                                               >  1:643949/1‑75 (MQ=11)
                                                                                                     aTAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTGCCGCGTCtt                                                           <  1:643947/89‑1 (MQ=255)
                                                                                                           tGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGATGc                                                                             <  1:1569167/66‑1 (MQ=25)
                                                                                                            gA‑TTTA‑GCGTGA‑TTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAATGCCTGATGc                                                                             <  1:1569168/64‑1 (MQ=14)
                                                                                                                       tGATTTT‑GTAGGT‑CGGATAAGGCGTTTTACGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTg                                                                    >  1:1569172/1‑65 (MQ=255)
                                                                                                                          tttt‑GTAGGT‑CGGATAA‑GCG‑TTTACGCCGCATCCGACATCAATGCCTGATGCGACGC‑TTGCCGCGTCTT‑ATCAGGCCTatctt                                            <  1:1569191/83‑5 (MQ=11)
                                                                                                                                                    tACGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTGCCGCGTCTTGATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCga  >  1:1569174/1‑103 (MQ=255)
                                                                                                                                                                        |                                                                                    
ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTG‑ATGCGACGC‑TTAACGCG‑TCTTA‑TCAGGCCTAC‑GCCAGACAGCGC‑AATAGCCTGA‑TTTA‑GCGTGATTTT‑GTAGGT‑CGGATAAGGCG‑TTTACGCCGCATCCGACATCAACGCCTGATGCGACGC‑TTGCCGCGTCTT‑ATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGG  >  NC_000913/4295903‑4296142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑