Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,818,781	C→A	intergenic (+197/‑93)	dinD → / → yicG	DNA damage‑inducible protein D/conserved inner membrane protein YicG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,818,781	0	C	A	97.6%	146.3 / ‑5.2	43	intergenic (+197/‑93)	dinD/yicG	DNA damage‑inducible protein D/conserved inner membrane protein YicG
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base A (21/20);  minor base . (0/1);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

CAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCC‑AAGGT‑CCAGGTG‑CAGTGAAC‑ACAATGGCT‑AAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑ACAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTC‑AAACACCCGG  >  NC_000913/3818546‑3819060                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                             cAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCC‑AAGGT‑CCAGGTG‑CAGTGAAC‑ACAATGGCT‑AAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCa                                                                                                                                                                                                                                                                    <  1:1500970/271‑1 (MQ=255)                                                   aaCTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCC‑AAGGTACCAGGTGACAGTGAACAACAATGGCT‑AAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCATGACAAGAAGTCAGAAGAAATGCATCTCTGCTACAG‑AAAT‑AGCG‑ATTTC‑ACAT‑AACGT‑AAAAG‑TTA‑TC‑AA‑tt                                                                                                                                                                                                                                                                                              >  1:1500982/1‑203 (MQ=255)                                                                  ttgaGACACCCGATGCGTAAGCC‑AAGGT‑CCAGGTG‑CA‑TGAAC‑ACAATGGCT‑AAATATTGCTACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGACAAATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑ttttt                                                                                                                                                                                                                   <  1:1500975/252‑1 (MQ=255)                                                                         acCCGATGCGTAAGCCAAAGGT‑CCAGGTG‑CAGTGAAC‑ACAATGGCTAAAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTATTTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TCAAA‑TTTCCCCTCCCCTTAAAAAAA‑T‑CTCAA‑‑CGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTa                                                                                                                                                                                                                  <  1:1500979/249‑1 (MQ=255)                                                                         acCCGATGCGTAAGCC‑AAGGT‑CCAGGTG‑CAGTGAAC‑ACAATGGCT‑AAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTa                                                                                                                                                                                                                  <  1:1500980/245‑1 (MQ=255)                                                                         acCCGATGCGTAAGCC‑AAGGT‑CCAGGTG‑CAGTG‑AC‑ACAATGGCT‑AAATATTGCTACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTa                                                                                                                                                                                                                  <  1:1500981/245‑1 (MQ=255)                                                                                    aaGCC‑AAGGT‑CCAGGTG‑CAGTGAAC‑ACAATGGCT‑AAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑‑TTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGcc                                                                                                                                                    >  1:1500985/1‑289 (MQ=255)                                                                                     aGCC‑AAGGT‑CCAGGTG‑CAGTGAAC‑ACAATGGCT‑AAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCcag                                                                                                                                                                                                                              >  1:1500986/1‑222 (MQ=255)                                                                                          aaGGT‑CCA‑GTG‑CAGTG‑AC‑ACAATGGCT‑AAATATTGCTACCTTTCTTTT‑CCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAG‑AAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑ttttt                                                                                                                                                                                                                   <  1:1500984/225‑1 (MQ=255)                                                                                                                                           ctttTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAACTACTCAATTCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGgcgtggc                                                                                                                                   >  1:1500989/1‑256 (MQ=255)                                                                                                                                                        gTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑TAAAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTTAAAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATg                                                                                                               <  1:1500988/266‑1 (MQ=255)                                                                                                                                                                        ttCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTg                                                                                                                                                                                                                                  >  1:1500992/1‑142 (MQ=255)                                                                                                                                                                                   tttCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGATTTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCC‑TGAC‑GGGGGCGCTGGCGGCCGGACG‑CGGCGCATGGATACA‑TTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGTCCAGTgc                                                   >  1:1500995/1‑295 (MQ=255)                                                                                                                                                                                    ttCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑TAAAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCACAAAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGCTT‑G‑TT‑ACACA‑TTTTGTA‑‑TTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACG‑CGGCGCATGGATAC‑TTTGGCGTAATTATTATTTGCGACCGCCa                                                                            >  1:592963/1‑272 (MQ=255)                                                                                                                                                                                    ttCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGcc                                                                                                                                                    >  1:1500996/1‑201 (MQ=255)                                                                                                                                                                                    ttCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCC‑TGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTg                                                    >  1:1500994/1‑293 (MQ=255)                                                                                                                                                                                    ttCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGAG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑‑TA‑TC‑AA‑TTtcccctc                                                                                                                                                                                                                                                                                       <  1:592959/82‑1 (MQ=255)                                                                                                                                                                                           gACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AATTTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCa                                                                                                                                                                  >  1:1500998/1‑182 (MQ=255)                                                                                                                                                                                            aCAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑‑TTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGgac                                                                                                                          <  1:1500990/218‑1 (MQ=255)                                                                                                                                                                                             cAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGgcg                                                                                                                                       <  1:1500991/205‑1 (MQ=255)                                                                                                                                                                                              aagaagTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑TAAAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTTAAAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCACAAAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACATTTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATATTctgctg                                     >  1:592968/1‑304 (MQ=255)                                                                                                                                                                                              aagaagTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑t                                                                                                                                                                                                                                                                                               <  1:592957/66‑1 (MQ=255)                                                                                                                                                                                               agaagTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑‑AAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCACAAAATCGCTACCCTGCCAGACAGA‑‑‑TTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGc                                                                              <  1:592967/258‑1 (MQ=255)                                                                                                                                                                                                 aagTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCGAATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCcaga                                                                                                                                                                                                                             <  1:592960/124‑1 (MQ=255)                                                                                                                                                                                                 aagTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTT‑CCCTCCCCTT‑AAAAAC‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATA‑Tctgct                                      >  1:592969/1‑294 (MQ=255)                                                                                                                                                                                                    gcAGAAG‑AATGCATCTCTGCTACAG‑AAAT‑AGCG‑A‑TTC‑ACAT‑AAC‑T‑A‑AAG‑TTA‑TC‑AA‑‑TTCCCCTCCCCTT‑‑AAAAA‑T‑CTCAA‑TCGt                                                                                                                                                                                                                                                              <  1:592966/83‑1 (MQ=255)                                                                                                                                                                                                      agaag‑aATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACATAAAC‑TAAAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TTAACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCC‑TGAC‑GGGGGCGCTgg                                                                                                                                   >  1:592970/1‑202 (MQ=255)                                                                                                                                                                                                         ag‑aaTGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGt                                                                                                                                                                                                                                                              <  1:592962/84‑1 (MQ=255)                                                                                                                                                                                                               gCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑ACAAG‑TTA‑Tc                                                                                                                                                                                                                                                                                                    <  1:592965/47‑1 (MQ=255)                                                                                                                                                                                                               gCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTg                                                                                                                                                                                <  1:1500997/149‑1 (MQ=255)                                                                                                                                                                                                               gCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑‑TA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑t‑ctc                                                                                                                                                                                                                                                                     <  1:592964/72‑1 (MQ=255)                                                                                                                                                                                                                    tctGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AATTTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGATTTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCC‑TGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGc                   >  1:1501002/1‑298 (MQ=255)                                                                                                                                                                                                                    tctGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGGCGCTGGCGGCCGGacgacg                                                                                                                      >  1:1501001/1‑201 (MQ=255)                                                                                                                                                                                                                    tctGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCC‑TGAC‑GGGGGCGCTGGCGGCCGGACGACGgc                                                                                                                    >  1:1501003/1‑201 (MQ=255)                                                                                                                                                                                                                      tGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑TAAAAAG‑TTAGTC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑‑TTTTAGGGAGAGAACCATGC‑TGG‑TT‑ACACA‑ttt                                                                                                                                                                                  >  1:592972/1‑142 (MQ=255)                                                                                                                                                                                                                      tGCTACAGAAAAT‑AGCG‑ATTTC‑AC‑T‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTC‑A‑TC‑TGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑t                                                                                                                                                                                             >  1:1501004/1‑128 (MQ=255)                                                                                                                                                                                                                           cAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGATTTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCA‑CGCAATTgg                                                                  >  1:1501005/1‑244 (MQ=255)                                                                                                                                                                                                                            aGAAAATAAGCG‑ATTTCAACAT‑AAC‑T‑AAAAGCTTA‑TC‑AA‑ATTCCCCTCCCCTT‑AAAAAA‑T‑CT‑AA‑‑CGTGACAAATGCGCAC‑AAATCGCTACCCTGCCAGACAGATTTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTATTTTGGTTGGCATTACTGCCGAAGCCATg                                                                                                                                                 <  1:592973/170‑1 (MQ=255)                                                                                                                                                                                                                              aaaaT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTC‑AAACACCCgg  >  1:1501006/1‑303 (MQ=255)                                                                                                                                                                                                                                aaT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATATTCTGCTggg                                   >  1:1501008/1‑269 (MQ=255)                                                                                                                                                                                                                                      cG‑ATTTC‑ACAT‑AAC‑T‑AAAAG‑TTA‑TCAAA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑‑TTTTA‑GGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGACGGGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGA‑GGT‑CAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTCAAAACACCCg   <  1:1501000/295‑1 (MQ=255)                                                                                                                                                                                                                                       g‑ATTTCAACAT‑AAC‑T‑AAAAGCTTA‑TCAAA‑TTTCCCCTCCCCTTAAAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACa                                                                                                         <  1:1501007/199‑1 (MQ=255)                                                                                                                                                                                                                                          tttC‑ACAT‑AAC‑T‑AAAAG‑‑TA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑‑GTTTAGGGAGAGAACCATGC‑T‑GTTT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTA‑TA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATATTCTGCTGGGc                                  <  1:592971/260‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                             CAGTTGGAAAACAGTGTAAAAATTACAGAGAAGAAATAGCAGAAACTAAAAACTCTTTTTGTTGATTGAGACACCCGATGCGTAAGCC‑AAGGT‑CCAGGTG‑CAGTGAAC‑ACAATGGCT‑AAATATTGC‑ACCTTTCTTTTCCCCCTCAGTTTTAACCTA‑TTTTTTCTTATGCATTTTCTCA‑GACAAGAAGTCAGAAG‑AATGCATCTCTGCTACAGAAAAT‑AGCG‑ATTTC‑ACAT‑AAC‑T‑ACAAG‑TTA‑TC‑AA‑TTTCCCCTCCCCTT‑AAAAAA‑T‑CTCAA‑TCGTGAC‑AATGCGCAC‑AAATCGCTACCCTGCCAGACAGA‑TTTTTAGGGAGAGAACCATGC‑T‑G‑TT‑ACACA‑TTTTGTA‑TTTGGTTGGCATTACTGCCGAAGCCATGAC‑GGGGGCGCTGGCGGCCGGACGACGGCGCATGGATACATTTGGCGTAATTATTA‑TTGCGACCGCCACCGCAATTGGCGGAGGGT‑CAGTGCGCGATATTCTGCTGGGCCACTATCCGCTCGGCTGGGTC‑AAACACCCGG  >  NC_000913/3818546‑3819060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑