| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 422,414 | Δ1 bp | intergenic (+55/‑104) | proY → / → malZ | putative transporter ProY/maltodextrin glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 422,412 | 0 | A | . | 77.3% | 20.6 / 7.1 | 22 | intergenic (+53/‑106) | proY/malZ | putative transporter ProY/maltodextrin glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base . (0/17); total (3/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-02 | |||||||||||
AAAGTGCCGGGTGGGGTAGC‑AACGACCATCGGC‑GGGCTGATTTTCCTGCTCTTTATTA‑TCGGGTTGATTGGTTATCA‑CCCGGATACG‑CGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGAAAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTG‑CCCCCATTTGTTAAACAAAGC‑AAAGATCAACTGCTCATTACACTGTGGCTGACGGGCG > NC_000913/422174‑422602 | aaaGTGCCGGGTGGGGTAGCAAACGACCATCGGC‑GGGCTGA‑TTTCCTGCTCTTTATTA‑TCGGGTTGATTGGTTATCA‑CCCGGATACG‑CGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTTAAAACGCCGCCACGA‑CGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa < 1:37097/310‑1 (MQ=255) aGC‑AACGACCATCGGCGGGGCTGATTTTCCTGCTCTTTATTA‑TCGGGTTGATTGGTTATCA‑CCCGGATACG‑CGTATCTCGCTGTA‑TGTCGGTTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TGTTTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGa > 1:407076/1‑225 (MQ=255) aCCATCGGC‑GGGCTGATTTTCCTGCTCTTTATTA‑TCGGGTTGATTGGTTATCACCCCGGATACGTCGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCCAAAATATCCTTCa < 1:407073/257‑1 (MQ=255) tgcttctTTATTAGTCGGGTTGATTGGTTATCA‑CCCGGATACG‑CGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑‑AACGCCGCCA‑GATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTa < 1:37100/299‑1 (MQ=255) atcggggTTGATTGGTTATCA‑CCCGGATACG‑CGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATTG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGAAAGTTA‑TCCG‑‑CCCCGTCCTCCTccca > 1:407082/5‑209 (MQ=255) tCGGGTTGATTGGTTATCA‑CCCGGATACG‑CGTATCTCGCTGTATTGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTCAAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGa < 1:37104/183‑1 (MQ=255) tGATTGGTTATCACCCCGGATACG‑CGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGG‑AATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc < 1:407078/239‑1 (MQ=255) tATCA‑CCCGGATACG‑CGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑‑AACGCCGCCACGATCGTC‑AGCTGGCTG‑AAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGG‑AATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑‑AATATCCTTCAGATGATGAGTGATCCTGCAt < 1:407080/222‑1 (MQ=255) tA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa < 1:407089/212‑1 (MQ=255) tA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGtt < 1:407090/249‑1 (MQ=255) tttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTa < 1:37109/242‑1 (MQ=255) cgcgTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTTAAAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc < 1:407091/200‑1 (MQ=255) tgtgCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑aaa < 1:407092/147‑1 (MQ=255) gATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGa > 1:407093/1‑108 (MQ=255) aTTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc < 1:1108406/177‑1 (MQ=255) aaaaCGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTc < 1:1046848/130‑1 (MQ=255) tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGG‑AATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑‑AATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTG‑CCCCCATTTGTTAAACAAAGC‑AAAGATCAACTGCTCAt < 1:407096/237‑1 (MQ=255) tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGG‑AATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTG‑CCCCCATTTGTTAAACAAAGC‑AAAGATCAACTGCTCATTa < 1:407095/236‑1 (MQ=255) aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGAACGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTG‑CCCCCATTTGTTAAACAAAGC‑AAAGATCAACTGCTCATTACACTGTGGCt < 1:37113/245‑1 (MQ=255) aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGAAAGTTA‑TCCG‑ccccgtc > 1:1207771/1‑68 (MQ=255) aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTACTCCGCCCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCCATTTGTTAAACAAAGCAAAAGATCAACTGCTc < 1:1060091/225‑1 (MQ=255) cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGAAAGTTA‑Tcc > 1:407100/1‑60 (MQ=255) aGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCG‑AAGTTA‑TCCG‑‑CCCCGTCCTCCTCCCC‑‑‑AATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTtcatca < 1:407099/126‑1 (MQ=255) gaggaAATCCTGATTCCGGCTTATTCCG‑AAGTTA‑TCCGCCCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATTCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTG‑CCCCCATTTGTTAAACAAAGC‑AAAGATCAACTGCTCATTACACTGTGGCTGACGGGCg < 1:37114/220‑1 (MQ=255) ttCCGAAAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTtcat < 1:1072031/96‑1 (MQ=255) | AAAGTGCCGGGTGGGGTAGC‑AACGACCATCGGC‑GGGCTGATTTTCCTGCTCTTTATTA‑TCGGGTTGATTGGTTATCA‑CCCGGATACG‑CGTATCTCGCTGTA‑TGTCGG‑TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGA‑TG‑TTT‑AAACGCCGCCACGATCGTC‑AGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGA‑TCCGGC‑TATTCCGAAAGTTA‑TCCG‑CCCCCGTCCTCCTCCCCC‑AAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGA‑TCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTG‑CCCCCATTTGTTAAACAAAGC‑AAAGATCAACTGCTCATTACACTGTGGCTGACGGGCG > NC_000913/422174‑422602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |