| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,385,621 | Δ1 bp | coding (111/1062 nt) | ycjF → | conserved inner membrane protein YcjF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,385,618 | 0 | T | . | 79.2% | 37.7 / ‑0.6 | 24 | coding (108/1062 nt) | ycjF | conserved inner membrane protein YcjF |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base . (7/12); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAATTTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCA‑‑GGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTG > NC_000913/1385400‑1385840 | gCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGC‑AAATTTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGgaa < 1:1056163/251‑1 (MQ=255) ccAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGATAAAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCTAAAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGt < 1:524885/315‑1 (MQ=255) ttGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAA‑‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGt < 1:101351/307‑1 (MQ=255) tGAGGCATTTCGTCCGCAGGTGATGGA‑GTCGACAAACCACTACCGCATA‑TCGTCTTGATGCTGCGCTGG‑ATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAA‑‑TTTG‑CCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGt < 1:101352/302‑1 (MQ=255) gatggatgTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAgg > 1:524888/1‑222 (MQ=255) gatggatgTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAA‑‑‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑Tgg < 1:101354/283‑1 (MQ=255) cactatccGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGt < 1:524889/230‑1 (MQ=255) tGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAATTTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGaagaag < 1:524892/149‑1 (MQ=255) cGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAATTTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑Ga < 1:524893/160‑1 (MQ=255) aCCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAA‑‑‑TTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGCGGGTAAT‑GGACGCAGCG‑TACGTCCG‑‑AACGCAGCCTGATGGCGC‑AAA‑TGGTGAT‑GGGAGGCG‑TGGCTCTGt > 1:524895/1‑225 (MQ=255) aaCCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑‑TTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAATGGGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGC‑AAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTTG‑GGCGCAAGCGTTGTC‑‑GGCA‑‑ggg > 1:524896/1‑248 (MQ=255) gATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑‑TTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTGATGGCGC‑AAA‑TGGTGATGGGGAGGGCGTGGCTCTGTTTTGGGCGCAAGCGTTGTC‑GGGCAGGGGGTGTACAGTGGACAATGAATGCCt > 1:524898/1‑257 (MQ=255) tGGAGGTCGATCAGAATCCT‑AAATTCAGGGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGTGATGGGGAGGGC‑TGGCTCTG‑TT‑‑GGCGCAAGCGTTGTCGGGGCAG‑GGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTg > 1:524901/1‑255 (MQ=255) tGGAGGTCGATCAGAATCCT‑AAATTCAGGGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑‑TTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGCGGGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGC‑AAA‑TGGTGATGGGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCAG‑GGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTgg > 1:524900/1‑255 (MQ=255) tGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCA‑‑GGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAgg < 1:101358/248‑1 (MQ=255) tGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACC‑TTGACG‑‑AAATCAGG‑CGCAA‑‑‑‑TT‑CCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGC‑AAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑‑GGCA‑‑GGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTg < 1:101359/278‑1 (MQ=255) tCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑gg < 1:524899/113‑1 (MQ=255) aTCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAA‑‑‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCA‑‑GGGTGTACAGTGGACaatg < 1:101360/212‑1 (MQ=255) ttCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCGAAAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑ggg < 1:1176635/186‑1 (MQ=255) ggCGCAGCAAGACCTTTGACA‑AAATTCAGGACGCAAAATTTTGCCCCGG‑CCACG‑CTCGAC‑GAAGCAGCAGgaaga < 1:524902/75‑1 (MQ=255) gc‑agACCTTTGACG‑AAAATCAGG‑CGCAAAA‑TTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑gg < 1:101361/139‑1 (MQ=255) gACCTTTGACG‑AAAATCAGG‑CGCAAAA‑‑TTG‑CCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTCGGAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGC‑AAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCA‑‑GGGTGTACAGTGGACAATGAATGCCTGGCaaa > 1:101363/1‑203 (MQ=255) ccttttGACGAAAAATCAGG‑CGCAAAATTTTGCCCCGGCCCACGCCTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACg > 1:524904/4‑94 (MQ=255) aCCTTTGACG‑AAAATCAGG‑CGCAA‑‑‑‑TT‑‑CCCGG‑CCACG‑CTCGACGGAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAAGTGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCA‑‑‑GGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCAtt < 1:101362/243‑1 (MQ=255) caaaaTTTGCCCCGG‑CCACG‑CTCGAC‑G‑AGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCGAAAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCA‑‑g < 1:524905/151‑1 (MQ=255) ttttGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCAG‑GGGTGTACAGTGGACaatg > 1:524907/1‑167 (MQ=255) | GCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATAGGAGAT‑AAATTGCGATGACCGAACCGTTAAAACCACGTATTGATTTCGACGGTCCTCTGGAGGTCGATCAGAATCCT‑AAATTCA‑GGGCGCAGC‑AGACCTTTGACG‑AAAATCAGG‑CGCAAAATTTTGCCCCGG‑CCACG‑CTCGAC‑GAAGC‑GCAGGAAGAAGAGGGGCAAGTC‑GAAGC‑GGTAAT‑GGACGCAGCGTTACGTCCG‑AAACGCAGCCTG‑TGGCGCAAAA‑TGGTGAT‑GGGAGGGC‑TGGCTCTGTTT‑‑GGCGCAAGCGTTGTC‑GGGCA‑‑GGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTG > NC_000913/1385400‑1385840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |