| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,282,845 | (T)6→5 | intergenic (+44/+95) | rplY → / ← yejK | 50S ribosomal subunit protein L25/nucleoid‑associated protein YejK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,282,840 | 0 | T | . | 84.2% | 27.9 / ‑1.8 | 20 | intergenic (+39/+100) | rplY/yejK | 50S ribosomal subunit protein L25/nucleoid‑associated protein YejK |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base . (8/8); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCC‑‑GGCAAT‑CAT‑CTAC‑GGTGGCAAAG‑AAGCGCCGCTGGCTATCGAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑AAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGAT‑CCCAGAAGATGCGTTCACCTAACAGCATC‑GCATC‑AAAGTTAATAGTCAAGCCACCGC > NC_000913/2282552‑2283082 | gggTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCC‑‑GGCAAT‑CAT‑CTAC‑GGTGGCAAAG‑AAGCGCCGCTGGCTATCGAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAA‑CCCGCTCCGGCGGGGt > 1:634775/1‑288 (MQ=255) gTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCC‑‑GGCAAT‑CAT‑CTACGGGTGGCAAAGAAAGCGCCGCTGGCTATCGAG‑C‑TGGATCACGACAAAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCG‑TGTTGACGGTAAAGAAATC‑AAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACgg < 1:161368/313‑1 (MQ=255) cGTGCCGCTAACAAGTTCCC‑‑GGCAAT‑CAT‑CTAC‑GGTGGCAAAG‑AAGCGCCGCTGGCTATCGAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACg > 1:634778/1‑294 (MQ=255) ccGCTAACAAGTTCCCCGGGCAATACATCCTAC‑GGTGGCAAAGAAAGCGCCGCTGGCTATCGAG‑CATGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGTAAAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACgg < 1:161371/298‑1 (MQ=255) aag‑aagCGCCGCTGGCTATCGAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTT‑AAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGGtttttt < 1:634780/228‑1 (MQ=255) aagCGCCGCTGGCTATCGAGTC‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACaa > 1:634785/1‑314 (MQ=255) agCGCCGCTGGCTATCGAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGGTTTTTTGt < 1:634783/227‑1 (MQ=255) agCGCCGCTGGCTATCGAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑‑AAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGAtt > 1:634786/1‑246 (MQ=255) gAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAA‑CCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGATTAAGGCG‑TTCACGCCGCATCGTGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑AAATTc > 1:634787/1‑349 (MQ=255) c‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAA‑CCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTA‑TTTTTGCA‑GCCAGATTAAGGCGTTTCACGCCGCATC‑TGGCATTCA‑GAACACAAAAc > 1:634788/1‑284 (MQ=255) aCAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAG‑AATCAAAGTT‑AAGCTCAGGA‑CGTCACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAA‑TGCTGAAT‑AAGTTGT‑‑AAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑AAATTCGGCGGCGTGCCTTTAATGGTc < 1:634791/317‑1 (MQ=255) cAGCGAAGTTCTGACCATCGTTGTTGACGGT‑AAG‑AATC‑AAGTT‑AAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTAC‑AACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑AAATTCGGCGGCGTGCCTTTAATGGTc < 1:634790/314‑1 (MQ=255) gaaTCAAAGTT‑AAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑‑AAAAACCCCGCTCCGGCGGG‑‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTA‑TTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑AAATTCGGCGGCGTGCCTTTAATGGTCa < 1:634793/280‑1 (MQ=255) tAAAGCTCAGGATCGT‑ACAGCGTCTACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑‑AAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTGACAGATTATG‑CCTTGATTACGGTAATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCACGAACACaa > 1:634794/1‑206 (MQ=255) tCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAAGTTGTAAAAAAACCCCGCTCCGGCGGGG‑GTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑T‑GCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑AAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCg < 1:1008661/278‑1 (MQ=255) gCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑‑AATTCGGCGGCGTGCC‑TTAATGGTCAAGGTATCGGTTGCTGGAT‑CCCAGAAGATGCGTTCAct > 1:634797/1‑291 (MQ=255) aCCCGTACAAACCGAAGCTGCAGCACATCGACTTCG‑TCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCG‑‑‑‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑T‑GCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAG‑CGTcc < 1:634796/198‑1 (MQ=255) tACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGGGTTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCa < 1:1102417/193‑1 (MQ=255) cAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTGCCAATTGGTCGCGC‑AAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTg > 1:634798/1‑262 (MQ=255) cAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAA‑CCCGCTCCGGCGGGG‑‑TTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTA‑TTTTTGCAGGCCAGATTAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTGACCGCCAGACGTCCGGCGCTGCCAATTGGTCGCGC‑AAATTCGGCGGCGTGCC‑TTAATGGTCAAGGTATCGGTTGCTg > 1:634799/1‑259 (MQ=255) ttcgCGCTTAATTGCTGAATAAAGTTGT‑AAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTG‑CAGATTATGTCC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGCAAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTgg < 1:634800/236‑1 (MQ=255) ggcggGG‑TTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTA‑TTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGCAAAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGATCCCCAGAAGATGCGTTCACCTAACAGCATCAGCATCAAAAGTTAATAGTCAAGCCAccgc < 1:634801/252‑1 (MQ=255) | GGGTAAGGGTGCGAGCCGCCGCCTGCGTGCCGCTAACAAGTTCCC‑‑GGCAAT‑CAT‑CTAC‑GGTGGCAAAG‑AAGCGCCGCTGGCTATCGAG‑C‑TGGATCACGAC‑AAAGTCATGAACATGCAAGCTAAAGCTGAATTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGA‑CGT‑ACAGCGTC‑ACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAAT‑AAGTTGT‑AAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTG‑CAGATTATG‑CC‑TGATTACGGT‑ATTGCTATTTTTTGCAGGCCAGA‑TAAGGCG‑TTCACGCCGCATC‑TGGCATTCA‑GAACACAAAACCGATTAATTG‑CCGCCAGACGTCCGGCGCTG‑CAATTGGTCGCGC‑AAATTCGGCGGCGTGCCTTTAATGGTCAAGGTATCGGTTGCTGGAT‑CCCAGAAGATGCGTTCACCTAACAGCATC‑GCATC‑AAAGTTAATAGTCAAGCCACCGC > NC_000913/2282552‑2283082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |