Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,840,930	0	A	.	59.5%	2.9 / 37.1	37	coding (1407/1458 nt)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base A (15/0);  new base . (0/22);  total (15/22)
Fisher's exact test for biased strand distribution p-value = 1.07e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCTTATCGCCAGTCTTATCAGCGG‑TTTTATTTGCGGC‑GC‑GGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTGACCACTGTCGATATGAT‑CCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATG‑ACGAGTTTTATCCCAGCCA  >  NC_000913/2840687‑2841180                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                     gCTTATCGCCAGTCTTATCAGCGG‑TTTTATTTGCGGC‑GC‑GGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCG‑CGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAaagaa                                                                                                                                                                                                                                <  1:702665/278‑1 (MQ=255)                tATCAGCGGTTTTTATTTGCGGCGGCGGGTTGCCGGTA‑GGCGGGGCTTGCCAGCCACTCAA‑GGCAGCG‑CGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAaag                                                                                                                                                                                                                                  <  1:198308/262‑1 (MQ=255)                tATCAGCGG‑TTTTATTTGCGGC‑GC‑GGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAaag                                                                                                                                                                                                                                  <  1:198309/262‑1 (MQ=255)                 aTCAGCGG‑TTTTATTTGCGGC‑GC‑GGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑TCCCTC‑ACACTGTTGTC‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCGAAAAGTATC‑AGAGCGTACAAACCGACCGTCGCCAAAGAAGTAAAGTCTT‑aa                                                                                                                                                                                                                   >  1:198310/1‑280 (MQ=255)                                               gCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑aa                                                                                                                                                                                                                                                  >  1:198313/1‑219 (MQ=255)                                               gCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGgc‑gc                                                                                                                                                              >  1:198314/1‑298 (MQ=255)                                                        gCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑Aaccg                                                                                                                                                                                                                                               >  1:198315/1‑213 (MQ=255)                                                                 gccagccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCG‑ATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAG‑AAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCaa                                                                                                                                                  <  1:702671/289‑1 (MQ=255)                                                                        aCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAg                                                                                                                                                                                               <  1:198316/241‑1 (MQ=255)                                                                            aaTGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTGCCCCAGGCGCGAAAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAaagaag                                                                                                                                                                                                                               >  1:198317/1‑212 (MQ=255)                                                                                 cAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAaag                                                                                                                                                                                                                                  <  1:702673/200‑1 (MQ=255)                                                                                                  aCCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTTATGGGGCGGC‑GCGCTTgccgcc                                                                                                                                                    <  1:702676/256‑1 (MQ=255)                                                                                                           cAG‑TTCTTCGATCCGGCGAATCC‑ATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAc                                                                                                                   <  1:702677/279‑1 (MQ=255)                                                                                                                 cttcGATCCGGCGAATCCAATGA‑GCATCGTCTGGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTA‑TTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTg                                                                                                                           >  1:702684/1‑268 (MQ=255)                                                                                                                 cttcGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGaaa                                                                                                                                                                                                      >  1:702683/1‑196 (MQ=255)                                                                                                                 cttcGATCCGGCGAATCC‑ATGAGGCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGaa                                                                                                                                                                                                       <  1:702678/193‑1 (MQ=255)                                                                                                                   tcGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAGT‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑aa                                                                                                                <  1:702679/275‑1 (MQ=255)                                                                                                                       tCCGGCG‑ATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTATT‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAG‑AAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTg                                                                                                         <  1:702680/277‑1 (MQ=255)                                                                                                                              agtCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAGT‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑a                                                                                                                 <  1:702681/260‑1 (MQ=255)                                                                                                                                 ccAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCA‑GCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTGAc                                                                                                       <  1:198318/268‑1 (MQ=255)                                                                                                                                  cAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AA‑CGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTGAc                                                                                                       <  1:702685/267‑1 (MQ=255)                                                                                                                                                        ttCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAaag                                                                                                                                                                                                                                  <  1:702686/132‑1 (MQ=255)                                                                                                                                                               tCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAG‑AAG‑tttttt                                                                                                                                                                           <  1:702687/174‑1 (MQ=255)                                                                                                                                                                cATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTGACCACTg                                                                                                  >  1:198319/1‑247 (MQ=255)                                                                                                                                                                          gCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑‑TTTTTAT‑GGGCGGC‑GCGCTTGCCGCCAACCAGTCTg                                                                                                                                          <  1:702689/195‑1 (MQ=255)                                                                                                                                                                          gCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑a                                                                                                                 <  1:702688/220‑1 (MQ=255)                                                                                                                                                                                     gTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑CTTCGGC‑TTTGAAGGATATTCCTGTTGAGGAAGCGGCAT‑GCC‑‑GCGCG‑‑AAGTATCAAGAGCGTAA‑CAACGACCGTCGCC‑AAGAAGT‑AAGTCTTAAA‑TTGAGGATGAAAATGTCAGTATTTCCAG‑AAG‑ttttt                                                                                                                                                                            <  1:702691/153‑1 (MQ=255)                                                                                                                                                                                     gTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGCTTTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGaagt‑aag                                                                                                                                                                                                                          >  1:702692/1‑112 (MQ=255)                                                                                                                                                                                        g‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGCTTTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AATTTGAGGATGAAAATGTCa                                                                                                                                                                                                >  1:1212474/1‑134 (MQ=255)                                                                                                                                                                                        g‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTGCCCCAGGCGCGAAAAGTATC‑atgag                                                                                                                                                                                                                                                         >  1:198320/1‑77 (MQ=255)                                                                                                                                                                                          tttA‑T‑CCTCGACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAG‑AAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCC‑ACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTGACCACTGTCGATATGATCCCCACACGGCGAGCATCGAATGGCGGTGa                                                          <  1:702690/259‑1 (MQ=255)                                                                                                                                                                                            tA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCaa                                                                                                                                                  >  1:198321/1‑173 (MQ=255)                                                                                                                                                                                                            ttG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGA‑CGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGggcgg                                                                                                                                                                  >  1:198322/1‑144 (MQ=255)                                                                                                                                                                                                            ttG‑C‑TCGGC‑TTTG‑AGGATA‑TCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTA‑TTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑‑AAGGTCTGACCACTG‑CGATATGAT‑CCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGG‑AAAACGTTTTCAGTTGCGAGATGAACGAGTTTTATCCCAGCCa  >  1:702694/1‑297 (MQ=255)                                                                                                                                                                                                                        tttG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑‑CCAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAG‑AAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAAGGTCTGACCACTGTCGATATGATCCCCACACGGCGAGCATCGAATGGCGGTGaa                                                         <  1:702693/237‑1 (MQ=255)                                                                                                                                                                                                                                                      tG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGCTGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTGACCACTGTCGATATGAT‑CCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAAc                                        >  1:198324/1‑227 (MQ=255)                                                                                                                                                                                                                                                         gccAGGCGCG‑‑AAGTATC‑AGAGCGTAC‑AACCGACCG‑CGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCaa                                                                                                                                                  <  1:198323/117‑1 (MQ=255)                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                     GCTTATCGCCAGTCTTATCAGCGG‑TTTTATTTGCGGC‑GC‑GGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAG‑TTCTTCGATCCGGCGAATCCAATGA‑GCATCGTCT‑GGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCG‑TTTA‑T‑CCTC‑ACACTGTTG‑C‑TCGGC‑TTTG‑AGGATATTCCTGTTGAGGAAGCGGCTG‑CCCAGGCGCG‑AAAGTATC‑AGAGCGTAC‑AACCGACCGTCGCCAAAGAAGT‑AAGTCTT‑AA‑TTGAGGATGAAAATGTCAGTATTTCCAGAAAG‑TTTTTTATGGGGCGGC‑GCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGAC‑AAAGGTCTGACCACTGTCGATATGAT‑CCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATG‑ACGAGTTTTATCCCAGCCA  >  NC_000913/2840687‑2841180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑