Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,515,480	C→T	100%	G400S (GGC→AGC)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,515,480	0	C	T	100.0%	77.3 / NA	24	G400S (GGC→AGC)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATG  >  NC_000913/4515213‑4515742                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                        tgATTGTGTCCTCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCtgctgc                                                                                                                                                                                                                                                                    <  1:141554/272‑1 (MQ=255)                                                gTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGCGAGGGTGATGCGTTTGCCATGCCCCCGGTAGCTGCTGCGCAGGGGTTTGGGTG‑AGAAGCCCTGAATGTTAAAATTaattttggtgt                                                                                                                                                                                                                   >  2:183206/1‑264 (MQ=255)                                                                                       gacaggctgGGGGAGGTGTATTATCGCATTTCTTTCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGcga                                                                                                                                                                             <  1:312347/268‑1 (MQ=255)                                                                                       ccGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACGGGTAGCCCAGGCTCGCCAGCTTGcga                                                                                                                                                                             <  1:183206/273‑1 (MQ=255)                                                                                                      gtgtAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACACCAGAAGCGGt                                                                                                                                                             >  1:45309/1‑274 (MQ=255)                                                                                                                    ttCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTg                                                                                                                                               >  1:242825/1‑274 (MQ=255)                                                                                                                               attcattcAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑gg                                                                                                                                                                                                                                                              >  2:27216/1‑152 (MQ=255)                                                                                                                                  cattcaAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGtt                                                                                                                                                                                                                                                           <  1:27216/152‑1 (MQ=255)                                                                                                                                                                                             tGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATg                                                                                                                                                                                                                           >  2:367797/1‑125 (MQ=255)                                                                                                                                                                                                cTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATgctg                                                                                                                                                                                                                        <  1:367797/125‑1 (MQ=255)                                                                                                                                                                                                           cTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTgca                                                                   >  1:273208/1‑263 (MQ=255)                                                                                                                                                                                                                      gcACCCAGTAGTTACGCGGCGAGCGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTgg                                                                                                                                                                                                     >  1:237945/1‑122 (MQ=255)                                                                                                                                                                                                                      gcACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTg                                                                                                                                                                                >  1:117164/1‑143 (MQ=255)                                                                                                                                                                                                                         cccAGTAGTTACGCGGCGAGCGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAg                                                                                                                                                                                                  <  2:237945/122‑1 (MQ=255)                                                                                                                                                                                                                         cccAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGcga                                                                                                                                                                             <  2:117164/143‑1 (MQ=255)                                                                                                                                                                                                                         cccAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCcgc                                                                                                                  <  1:290329/202‑1 (MQ=255)                                                                                                                                                                                                                         cccAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGaa                                                      <  1:286827/262‑1 (MQ=255)                                                                                                                                                                                                                                          gagaGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTg                                                        <  1:347814/243‑1 (MQ=255)                                                                                                                                                                                                                                            gagGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTg                                                                                                                                                                                >  2:109747/1‑121 (MQ=255)                                                                                                                                                                                                                                               ggTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGcga                                                                                                                                                                             <  1:109747/121‑1 (MQ=255)                                                                                                                                                                                                                                                       gTTTGCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTg                                                        <  1:283681/230‑1 (MQ=255)                                                                                                                                                                                                                                                           gCCTTGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTg                                                           >  1:324255/1‑223 (MQ=255)                                                                                                                                                                                                                                                              ttGCTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTg                                                        <  2:324255/223‑1 (MQ=255)                                                                                                                                                                                                                                                                gCTCCAGGTAGCTGCGGCGCA‑GGTTTTGGTTGTAGACCCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGa    <  1:252502/273‑1 (MQ=255)                                                                                                                                                                                                                                                                 cTCCAGGTAGCTGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCCTGTCGGCTTCACCGTCGTAATCTTGCAGCCGGCCGTTGACGGGGGGCGCCCCATCCGGCCCCGATTTGCTTTTCAGCAACCggtggttggag  >  2:252502/1‑269 (MQ=255)                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                        CGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCA‑GGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAGCAGGCTGTTGAAGGTGTGCACCTCATCCGGCGCATATTTGCTTTTCAGCATCAGGTCGTCGATG  >  NC_000913/4515213‑4515742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑