| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,515,480 | C→T | 100% | G400S (GGC→AGC) | fecA ← | ferric citrate outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,515,480 | 0 | C | T | 100.0% | 93.1 / NA | 26 | G400S (GGC→AGC) | fecA | ferric citrate outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/12); total (14/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAG > NC_000913/4515264‑4515678 | cgcgGTCGTAAGTGCTTGAGCCGGACGGCAACTGCCCGCGGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTc < 1:141495/271‑1 (MQ=255) ggTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTctgg > 1:453748/1‑271 (MQ=255) gggCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTTAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCCTAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGGTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCGGGAACt > 1:430234/1‑271 (MQ=255) ggCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCg > 1:88673/1‑271 (MQ=255) gctgctGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCa > 2:383848/1‑188 (MQ=255) ctgctgGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAg < 1:383848/188‑1 (MQ=255) agtaACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAgg > 1:304963/1‑173 (MQ=255) gtaACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAggg < 2:304963/173‑1 (MQ=255) cGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGAACCCAGacggcgtcagacag > 2:87554/1‑259 (MQ=255) caAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGc > 2:130075/1‑137 (MQ=255) aaaTAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAgctgct < 1:130075/137‑1 (MQ=255) aGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATAATAAAGATCTGGCTGGAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATcc < 1:368202/272‑1 (MQ=255) cGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGgtgt > 1:87489/1‑129 (MQ=255) gTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTa < 2:87489/129‑1 (MQ=255) gTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATAc < 1:166529/220‑1 (MQ=255) aaGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAg > 1:346462/1‑200 (MQ=255) aGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGa < 2:346462/200‑1 (MQ=255) gCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGCGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCg > 1:318521/1‑210 (MQ=255) cTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGa < 2:318521/210‑1 (MQ=255) tAGCGTGTCTCAATCCCGCGCACCCAGTAGTTACGGGGCGGGGGGGTGATGCTTTGGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGATTTTTGAATTTATGCTGGCTGTCTGGCGGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTgcag < 1:87554/272‑1 (MQ=255) aCCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGTTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGa > 2:301422/1‑186 (MQ=255) aCCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGgcggcg > 1:9657/1‑195 (MQ=255) aCCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATc > 2:393598/1‑200 (MQ=255) cccAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATcc < 1:393598/200‑1 (MQ=255) cccAGTAGTTACGCGGCGAGAGGGTGATGCGGTTGCCTTGCTCCAGGTAGCTGTTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGAt < 1:301422/186‑1 (MQ=255) agagGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTg > 2:160646/1‑122 (MQ=255) gagGGTGATGCGTTTGCCTTGCTCCAGGTAGCTGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGc < 1:160646/122‑1 (MQ=255) | CGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGTAGCGTGGCTCAATACCGCGCACCCAGTAGTTACGCGGCGAGAGGGTGATGCGTTTGCCTTGCTCCAGGTAGCCGCTGCGCAGGGTTTGGGTGTAGAACCCCTGAATGTTGAATTTATGCTGGCTGTCTGGCTGGAACTGGTAGCCCAGGCTCGCCAGCTTGCGACGACCCCAGAAGCGGTCATACGGGCGGGTGGATTGCCAGCGATCGGCGTCGTAATCCGCGCGAGACAGGCCACCGGGCATGTCGGCTTCACCGTCGTAATATTGCAG > NC_000913/4515264‑4515678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |