Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,009,421	C→T	8.5%	H526H (CAC→CAT)	rlmL →	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,009,421	0	C	T	8.5%	146.1 / 4.0	47	H526H (CAC→CAT)	rlmL	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

GACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGCCTGATTCAGGCCGATTGCCTGGCGTGGCTGCGTGAGGCAAA  >  NC_000913/1009152‑1009660                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 gACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACGGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCg                                                                                                                                                                                                                                                <  1:443847/272‑1 (MQ=255)      ataagagacagaGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGNTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATc                                                                                                                                                                                                                                            <  1:251938/260‑1 (MQ=255)                   gAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATCCTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCt                                                                                                                                                                                                                              >  1:436277/1‑272 (MQ=255)                   gAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCCCACTTGTGGGTGAACCTGAAGGATAATCTCGATACTGGTCTGTTCCACGATCACCGCACCCCCcctcccacgcg                                                                                                                                                                                                                              >  2:191602/1‑265 (MQ=255)                           gccgccAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGa                                                                                                                                                                                                                      >  1:409932/1‑272 (MQ=255)                                                             cAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCa                                                                                                                                                                                                                                              >  2:303029/1‑214 (MQ=255)                                                              aGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCAt                                                                                                                                                                                                                                             <  1:303029/214‑1 (MQ=255)                                                                                    tGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGc                                                                                                                                                             <  1:191602/272‑1 (MQ=255)                                                                                    tGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGc                                                                                                                                                             <  1:361116/272‑1 (MQ=255)                                                                                                 gTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAg                                                                                                                                                 >  1:9930/1‑271 (MQ=255)                                                                                                               gcCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCt                                                                                                                                                                                                                              >  1:407061/1‑180 (MQ=255)                                                                                                               gcCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCaa                                                                                                                                                                                             >  1:68545/1‑213 (MQ=255)                                                                                                                ccAAACAAACTTGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAAc                                                                                                                                                                                            <  2:68545/213‑1 (MQ=255)                                                                                                                ccAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTc                                                                                                                                                                                                                             <  2:407061/180‑1 (MQ=255)                                                                                                                           ggTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTg                                                                                                                                                              >  1:153073/1‑232 (MQ=255)                                                                                                                           ggTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATTCGGGATTAGGCGGTGCCCGCGGCCACCA‑CACCGTg                                                                                                                      >  2:87535/1‑271 (MQ=255)                                                                                                                            gTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGc                                                                                                                                                             <  2:153073/232‑1 (MQ=255)                                                                                                                                tGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCAACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGat                                                                                                                   >  1:145555/1‑269 (MQ=255)                                                                                                                                    aaCCCGTAACCGCCAGAAGGGCAAACACCAAAACCGTAAACGGTGGTGGATGGGGGAGTTTCTTGAAGTGACCGAATATCACGCCACCTTGTGGGTGAACCTGACGGATTATCTCGTGACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTcgc                                                                                                            <  2:145555/272‑1 (MQ=255)                                                                                                                                            aaCGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCCACCTGTTCTCTTATCCCGGCAGCCCCCCCGTg                                                                                                                                                              >  2:343112/1‑215 (MQ=255)                                                                                                                                                                     ccAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATc                                                                                                   >  1:34668/1‑248 (MQ=255)                                                                                                                                                                      cAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCt                                                                                                  <  2:34668/248‑1 (MQ=255)                                                                                                                                                                                gCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCATCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACgcag‑c                                                                                                                                  >  2:393375/1‑206 (MQ=255)                                                                                                                                                                                gCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCATCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACgcag‑c                                                                                                                                  >  2:357139/1‑206 (MQ=255)                                                                                                                                                                                 cGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCATCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACgcag‑ca                                                                                                                                 <  1:393375/206‑1 (MQ=255)                                                                                                                                                                                 cGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCATCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACgcag‑ca                                                                                                                                 <  1:357139/206‑1 (MQ=255)                                                                                                                                                                                                             gAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCaa                                                                                                                                                                                             >  2:249871/1‑119 (MQ=255)                                                                                                                                                                                                              aaTATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAAc                                                                                                                                                                                            <  1:249871/119‑1 (MQ=255)                                                                                                                                                                                                                           cTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTc                                                                        >  1:72326/1‑221 (MQ=255)                                                                                                                                                                                                                            ttGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCt                                                                       <  2:72326/221‑1 (MQ=255)                                                                                                                                                                                                                             tgtgGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTcgcg                                                                                                           >  2:382564/1‑184 (MQ=255)                                                                                                                                                                                                                              gtgGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGt                                                                                                          <  1:382564/184‑1 (MQ=255)                                                                                                                                                                                                                                       ccTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGt                                                                                                               >  2:364768/1‑170 (MQ=255)                                                                                                                                                                                                                                        cTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTc                                                                                                              <  1:364768/170‑1 (MQ=255)                                                                                                                                                                                                                                         tGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACgcag‑c                                                                                                                                  >  1:213213/1‑149 (MQ=255)                                                                                                                                                                                                                                         tGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGt                                                                         >  1:358426/1‑206 (MQ=255)                                                                                                                                                                                                                                          gACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACgcag‑ca                                                                                                                                 <  2:213213/149‑1 (MQ=255)                                                                                                                                                                                                                                          gACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTc                                                                        <  2:358426/206‑1 (MQ=255)                                                                                                                                                                                                                                              gATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCa                                               >  2:49205/1‑227 (MQ=255)                                                                                                                                                                                                                                               aTTATCTCGTTACTGGTCTGTTCCTCGATCACCGCCTCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGCCTGATTCAGGCCGATTGCCTGGCGTGGCTGCGTGAGGCaaa  <  1:87535/271‑1 (MQ=255)                                                                                                                                                                                                                                               aTTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCAt                                              <  1:49205/227‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCg                                                                          >  2:82535/1‑181 (MQ=255)                                                                                                                                                                                                                                                                 tGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGt                                                                         >  2:229824/1‑182 (MQ=255)                                                                                                                                                                                                                                                                  gTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGt                                                                         <  1:82535/181‑1 (MQ=255)                                                                                                                                                                                                                                                                  gTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTc                                                                        <  1:229824/182‑1 (MQ=255)                                                                                                                                                                                                                                                                           tCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGcc                                                               >  2:40073/1‑182 (MQ=255)                                                                                                                                                                                                                                                                            cACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCt                                                              <  1:40073/182‑1 (MQ=255)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 GACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAAACCCGTGAACGCCAGAAGGGCAAAAACCAATACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAACGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGCATCGCCCGTCGTATGCTCGGTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGTGCACGCAG‑CACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGCCTGATTCAGGCCGATTGCCTGGCGTGGCTGCGTGAGGCAAA  >  NC_000913/1009152‑1009660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑