Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,636,200 C→A 15.1% R386L (CGC→CTC)  der ← 50S ribosomal subunit stability factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,636,2000CA15.1% 71.7 / 6.1 28R386L (CGC→CTC) der50S ribosomal subunit stability factor
Reads supporting (aligned to +/- strand):  ref base C (9/14);  new base A (2/2);  total (12/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

TCGCATACGGGTTTTCCCCTTCTTTG‑AACTGAATACGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGC  >  NC_000913/2635961‑2636418
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                            
tCGCATACGGGTTTTCCCTTTCTTTG‑ACCTGAATACGAAGCGGCGTGCCCATTACGTCGAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTg                                                                                                                                                                                             <  2:92384/272‑1 (MQ=255)
 ccctattctgTTTTCCCCTTCTTTGAAACTGATTACGATCCGGGGTTCCCATTACTGCCAGCGTTTTGCGGAAGTAGTTCAGCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCGGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTg                                                                                                                                                                                             <  2:45113/263‑1 (MQ=255)
                        tG‑AACTGAATACGAATCGGCGATCCATTTCCGTCCAGCGATTTGCGGAAGTAGTTCATCAATTACCGCTTGAAGGAATCAGGCAGTTCTTTCACCTGATTACCGTGAATCACCAAAATCGGGGGTTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACAAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  1:30690/217‑1 (MQ=255)
                                 atagctaTCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCGTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTTGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGAGGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCa‑aa                                                                                                                                                            <  1:374416/266‑1 (MQ=255)
                                             gATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGCGATCTTCAACAGCCATCGTCATGCTGCACGTCAGCAAAGCGGTCCCC‑CACAGACCGGGGGAGCTTGAATACCCTCAACGTACT‑AGTCACAACAAGTTAcacac                                                                                                                                                >  2:733548/1‑268 (MQ=255)
                                                               gATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTACCGTGCAAGGc                                                                                                                              >  1:712712/1‑272 (MQ=255)
                                                                      ggAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCAACGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGt                                                                                                                                                                                              >  1:526596/1‑202 (MQ=255)
                                                                      ggAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATAACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTAAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCCACAGCCATAGTCATGATGGGCGTCCGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCGTCACGTACTGATTCA‑AACAAATTACCAACACCACAGCCGTGCAAGGCCGAGATa                                                                                                                       >  1:1099166/1‑272 (MQ=255)
                                                                       gAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTg                                                                                                                                                                                             <  2:526595/202‑1 (MQ=255)
                                                                                             ttGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgc                                                                                                                                                                                                                              >  2:52650/1‑148 (MQ=255)
                                                                                              tGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  1:52650/148‑1 (MQ=255)
                                                                                                             ggTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgc                                                                                                                                                                                                                              >  2:372369/1‑132 (MQ=255)
                                                                                                              gTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  1:372370/132‑1 (MQ=255)
                                                                                                                            tACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAac                                                                                                                                                >  2:548125/1‑193 (MQ=255)
                                                                                                                             aCCGTGAATCACCACAATCGGCGGGTTATAACCACCTGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAaca                                                                                                                                               <  1:548126/193‑1 (MQ=255)
                                                                                                                                  gAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAac                                                                                                                                                >  1:534412/1‑187 (MQ=255)
                                                                                                                                   aaTCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAaca                                                                                                                                               <  2:534411/187‑1 (MQ=255)
                                                                                                                                    aTCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgc                                                                                                                                                                                                                              >  1:531612/1‑109 (MQ=255)
                                                                                                                                    aTCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGc                                                                                                                                   >  1:942813/1‑198 (MQ=255)
                                                                                                                                    aTCACCACAATCGGCGGGTTATAACCACCGCCGTGGGCATATTTCAGCTTCCCACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGt                                                                                                                                                                                              >  1:482527/1‑140 (MQ=255)
                                                                                                                                     tCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  2:531611/109‑1 (MQ=255)
                                                                                                                                     tCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTg                                                                                                                                                                                             <  2:482526/140‑1 (MQ=255)
                                                                                                                                     tCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCa                                                                                                                                  <  2:942812/198‑1 (MQ=255)
                                                                                                                                      caccacAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgc                                                                                                                                                                                                                              >  1:953440/1‑107 (MQ=255)
                                                                                                                                       accacAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  2:953439/107‑1 (MQ=255)
                                                                                                                                       accacAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgc                                                                                                                                                                                                                              >  1:1125325/1‑106 (MQ=255)
                                                                                                                                        ccacAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  2:1125324/106‑1 (MQ=255)
                                                                                                                                          ccatgcgtggggTTTTAACCACCGGCGTTGGCATATTTCAGCTTCACCCGCCGACCGCGTACCAGCGGCGGTTGGTGATCGTCAACAGCCATCGTCATGATGCGCTTCAGCATAGAGCTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCAc                                                   <  1:271277/263‑1 (MQ=255)
                                                                                                                                                                  gCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGGCATCGTCATGATgc                                                                                                                                                                                                                              >  1:754083/1‑79 (MQ=255)
                                                                                                                                                                  gCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgc                                                                                                                                                                                                                              >  2:891629/1‑79 (MQ=255)
                                                                                                                                                                  gCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAAcacc                                                                                                                                             >  2:981325/1‑158 (MQ=255)
                                                                                                                                                                   cGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGGCATCGTCATGATgcg                                                                                                                                                                                                                             <  2:754082/79‑1 (MQ=255)
                                                                                                                                                                   cGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCTTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAAcacca                                                                                                                                            <  1:981326/158‑1 (MQ=255)
                                                                                                                                                                   cGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  1:891630/79‑1 (MQ=255)
                                                                                                                                                                          atatTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgc                                                                                                                                                                                                                              >  2:112557/1‑71 (MQ=255)
                                                                                                                                                                           tatTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATgcg                                                                                                                                                                                                                             <  1:112557/71‑1 (MQ=255)
                                                                                                                                                                                     tCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGAGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTc                                                                                                                                                                >  1:143204/1‑121 (MQ=255)
                                                                                                                                                                                      cacaCGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGAGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCa                                                                                                                                                               <  2:143204/121‑1 (MQ=255)
                                                                                                                                                                                           gacgacCTCTTACCAGTGGCGGTTGTTTATCTTCAACAGCCATCGTCATGATGCGCGTAAGCATAGAGGTCCCCACAC‑GACGGGTGTAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCACAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAgcgc  <  1:733549/272‑1 (MQ=255)
                                                                                                                                                                                                 cgcgTACCAGCGGCGGTTGGTGATCGTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACGGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCtt                                                       <  1:452836/213‑1 (MQ=255)
                                                                                                                                                                                                      aCCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGAGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATc                                                                                                   >  1:942427/1‑164 (MQ=255)
                                                                                                                                                                                                       ccAGCGGCGGTTGGTGATCTTCATCAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTTATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctttc                                                                 <  1:490709/197‑1 (MQ=255)
                                                                                                                                                                                                       ccAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGAGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCg                                                                                                  <  2:942426/164‑1 (MQ=255)
                                                                                                                                                                                                                                     tCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAg                                                                                            >  1:28349/1‑140 (MQ=255)
                                                                                                                                                                                                                                      cGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGc                                                                                           <  2:28349/140‑1 (MQ=255)
                                                                                                                                                                                                                                               gcgcGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATaa                                                                                              >  1:175924/1‑128 (MQ=255)
                                                                                                                                                                                                                                                cgcgTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATaaa                                                                                             <  2:175924/128‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                            
TCGCATACGGGTTTTCCCCTTCTTTG‑AACTGAATACGAATCGGCGATCCCATTACGTCCAGCGATTTGCGGAAGTAGTTCATCAAGTAGCGCTTGTAGGAATCAGGCAGGTCTTTCACCTGATTACCGTGAATCACCACAATCGGCGGGTTATAACCACCGGCGTGGGCATATTTCAGCTTCACACGACGACCGCGTACCAGCGGCGGTTGGTGATCTTCAACAGCCATCGTCATGATGCGCGTCAGCATAGAGGTCCCCACAC‑GACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCA‑AACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGC  >  NC_000913/2635961‑2636418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑