Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,664,265 C→G 6.3% S118S (TCG→TCC)  gadW ← DNA‑binding transcriptional dual regulator GadW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,664,2650CG 6.3% 216.8 / 6.7 64S118S (TCG→TCC) gadWDNA‑binding transcriptional dual regulator GadW
Reads supporting (aligned to +/- strand):  ref base C (32/27);  new base G (2/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

TTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGT‑GAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAAT  >  NC_000913/3663994‑3664531
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                           
tttCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAAAAAACATc                                                                                                                                                                                                                                                                             >  1:268747/1‑272 (MQ=255)
  tccgacatatTATGCATGGGAATTTGACGTAACTGGAGTATTCGGCTGGCCATCGACATCCTTGAGGCGAGTAATATTTTACCGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGa                                                                                                                                                                                                                                                                           <  2:268746/266‑1 (MQ=255)
                      aaTTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCTTTCAACTTTTCCTGAACCAGTGCTGATGCTATTGAAATGTAAGGGGAACAGTTCTTTCTTATGATACAACAGCAAAAAAAAGGAAAGGGagagaa                                                                                                                                                                                                                                                       >  2:281175/1‑270 (MQ=255)
                                                                       aGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAACGGTAGAGc                                                                                                                                                                                                                                                        >  2:665605/1‑222 (MQ=255)
                                                                       aGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGc                                                                                                                                                                                                                                                        <  1:665606/222‑1 (MQ=255)
                                                                          aaTATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATTAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCACAGTATCGGTGAATTTAACCCGGATTCATTGAAGTTAAGAAAAAt                                                                                                                                                                                                   >  2:821112/1‑272 (MQ=255)
                                                                          aaTATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGCGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCCCAGTATCGGGTAATTTACCCCGGATTCCATTAAGATCTCaaa                                                                                                                                                                                                      >  2:705964/1‑269 (MQ=255)
                                                                          aaTATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGAAAAACAGGAAAGGTAGAGCATATTGCCCAGTAACGGTGAATTTACCCCGGATTCAATTAAGCAATCaaaaaa                                                                                                                                                                                                   >  2:221794/1‑271 (MQ=255)
                                                                                    tGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGc                                                                                                                                                                                                                                                        >  2:543522/1‑209 (MQ=255)
                                                                                    tGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGc                                                                                                                                                                                                                                                        <  1:543523/209‑1 (MQ=255)
                                                                                                       cTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACt                                                                                                                                                                      >  1:254624/1‑272 (MQ=255)
                                                                                                                           gACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCt                                                                                                                                                  <  1:221794/272‑1 (MQ=255)
                                                                                                                                    tGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTagag                                                                                                                                                                                                                                                         >  2:881734/1‑160 (MQ=255)
                                                                                                                                    tGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAGGGGGGGTGAATGGTTAATTAAAAACAGGTCttgtcagct                                                                                                                                          >  2:435710/1‑264 (MQ=255)
                                                                                                                                     ggTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGc                                                                                                                                                                                                                                                        <  1:881735/160‑1 (MQ=255)
                                                                                                                                     ggTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACa                                                                                                                                                          <  1:435711/254‑1 (MQ=255)
                                                                                                                                              tCTTTCCGCGATATCGCGCAGATACCATCGGTGGGCGATAGCAAAGCTAATCAGGCGTTCAACTTATACTGAGACAGTGCTGATGCTATGTAAAAGTAAGGGGATCATTTCTTTCTTAAGAGAAAACATCGAGAAACAGGAAAGGTAGAGCATAtt                                                                                                                                                                                                                                                   <  1:1128249/156‑1 (MQ=255)
                                                                                                                                               cTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATc                                                                                                                                                                                                                                                                             >  1:104706/1‑129 (MQ=255)
                                                                                                                                                tttCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCg                                                                                                                                                                                                                                                                            <  2:104706/129‑1 (MQ=255)
                                                                                                                                                   ccGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTTCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGCAAAAATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTAACCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGTGGTGACTGGTTAATTCACAAACGGTCATTGCTGTGTAACCCTAAATTGCGt                                                                                                                          >  1:399495/1‑272 (MQ=255)
                                                                                                                                                   ccGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATGGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCCGCGGCCTGGGGGGGGACTGGTTAATTAACAAACGGTCTTTGCTGTTTTACTCTAAAtcggtt                                                                                                                          >  2:330789/1‑267 (MQ=255)
                                                                                                                                                   ccGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATGGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgt                                                                                                                                           <  1:330799/255‑1 (MQ=255)
                                                                                                                                                   ccGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATGGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgt                                                                                                                                           >  2:330798/1‑255 (MQ=255)
                                                                                                                                                   ccGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGCCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTTTTTGCTGTGTAACACAAAAGTGGGt                                                                                                                          >  2:789312/1‑272 (MQ=255)
                                                                                                                                                    cgcgATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATGGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgt                                                                                                                                           <  1:330790/254‑1 (MQ=255)
                                                                                                                                                    cgcgATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAgg                                                                                                                                                                                                                                                              >  1:903424/1‑139 (MQ=255)
                                                                                                                                                    cgcgATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCg                                                                                                                                                                                     <  2:841519/212‑1 (MQ=255)
                                                                                                                                                    cgcgATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCg                                                                                                                                                                                     >  1:841520/1‑212 (MQ=255)
                                                                                                                                                     gcgATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAGAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCAACAGCGGCATGGGGGGGGACTGGTTAATTAACAAAAGGTCTTTTCTGTGTAAccccccgttgcctaa                                                                                                                        >  2:1091219/1‑260 (MQ=255)
                                                                                                                                                     gcgATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGt                                                                                                                                                                                                                                                             <  2:903423/139‑1 (MQ=255)
                                                                                                                                                      cgATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAGAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCt                                                                                                                                                  <  1:1091220/245‑1 (MQ=255)
                                                                                                                                                                        aaCGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCg                                                                                                                                                                                              >  1:379936/1‑183 (MQ=255)
                                                                                                                                                                         aCGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGt                                                                                                                                                                                             <  2:379935/183‑1 (MQ=255)
                                                                                                                                                                                gCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAACACATCGATaaa                                                                                                                                                                                                                                                                       >  1:820454/1‑102 (MQ=255)
                                                                                                                                                                                 cGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAAc                                                                                                                                                                                                                                                                      <  2:820453/102‑1 (MQ=255)
                                                                                                                                                                                 cGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGaaa                                                                                                                                                                                                                                                                >  2:704922/1‑108 (MQ=255)
                                                                                                                                                                                  gATATTAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATg                                                                                             <  1:821113/270‑1 (MQ=255)
                                                                                                                                                                                  gATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAg                                                                                                                                                                                                                                                               <  1:704923/108‑1 (MQ=255)
                                                                                                                                                                                  gATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATg                                                                                             <  1:705965/270‑1 (MQ=255)
                                                                                                                                                                                                     cGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGat                                                                        <  1:789313/272‑1 (MQ=255)
                                                                                                                                                                                                         cAACTTTTCCTGAAACAGTGCTGACGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAAAATTTTGTAAGTTAAGGCCAACTGTTAGCTCAACGatata                                                                     >  2:76048/1‑270 (MQ=255)
                                                                                                                                                                                                          aaCTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGttaatta                                                                                                                                                             >  1:64797/1‑182 (MQ=255)
                                                                                                                                                                                                          aaCTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtg                                                                                                                                            >  2:978020/1‑199 (MQ=255)
                                                                                                                                                                                                          aaCTTTTCCTGAAACAGTGCTGACGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                                                                    <  1:76048/255‑1 (MQ=255)
                                                                                                                                                                                                           aCTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGttaattaa                                                                                                                                                            <  2:64797/182‑1 (MQ=255)
                                                                                                                                                                                                           aCTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgt                                                                                                                                           <  1:978021/199‑1 (MQ=255)
                                                                                                                                                                                                                              tGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTAt                                                                                                                                                                                                                                          >  1:610298/1‑85 (MQ=255)
                                                                                                                                                                                                                              tGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCAc                                                                                                                                                                                          >  1:952636/1‑133 (MQ=255)
                                                                                                                                                                                                                              tGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAAc                                                                                                                                                           >  1:859398/1‑164 (MQ=255)
                                                                                                                                                                                                                              tGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTAATCGATAACCAGTCGTtgctttaacaggtga                                               >  2:231511/1‑260 (MQ=255)
                                                                                                                                                                                                                               gATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATc                                                                                                                                                                                                                                         <  2:610297/85‑1 (MQ=255)
                                                                                                                                                                                                                               gATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCAcc                                                                                                                                                                                         <  2:952635/133‑1 (MQ=255)
                                                                                                                                                                                                                               gATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACa                                                                                                                                                          <  2:859397/164‑1 (MQ=255)
                                                                                                                                                                                                                                        gAAAAGTAAGGGGATCAGTTTTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGc                                                                                                                                              >  1:420372/1‑167 (MQ=255)
                                                                                                                                                                                                                                         aaaaGTAAGGGGATCAGTTTTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCt                                                                                                                                             <  2:420371/167‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTagag                                                                                                                                                                                                                                                         >  1:21124/1‑49 (MQ=255)
                                                                                                                                                                                                                                                   ggATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGc                                                                                                                                              >  1:1115111/1‑156 (MQ=255)
                                                                                                                                                                                                                                                    gATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGc                                                                                                                                                                                                                                                        <  2:21124/49‑1 (MQ=255)
                                                                                                                                                                                                                                                    gATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCt                                                                                                                                             <  2:1115110/156‑1 (MQ=255)
                                                                                                                                                                                                                                                          ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTAAGCTCATCGATATCCAGTCGTCGCGTATCCGGT‑GAACAAAACGCAAAATCGACTGCCAAAtttt                  >  1:766879/1‑272 (MQ=255)
                                                                                                                                                                                                                                                               tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGT‑GAACAAAACGCAAAATCGTCTGCCAAATTTTtctc              >  1:576348/1‑271 (MQ=255)
                                                                                                                                                                                                                                                                cttATGAGAAAACATAGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGTAAACTGTAAGCTCATCGATATCCAGTCGTCGCGTATCCGGT‑GAACAAAACGCAAAATCGTCTGCCAAATTTTtct               >  1:582417/1‑269 (MQ=255)
                                                                                                                                                                                                                                                                  tATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCCCACCCATATCCAGTCGTCGCGTACCCGGTGGAACAAAAGACAAAACCGCCGGCAAATTTTTactc              >  2:50240/1‑269 (MQ=255)
                                                                                                                                                                                                                                                                       gaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCg                                                                                                                                                                                              >  2:1020918/1‑88 (MQ=255)
                                                                                                                                                                                                                                                                        aaaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGt                                                                                                                                                                                             <  1:1020919/88‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGAATGGTTAATTAACAAACGGTCTTTGCTGTTTAACCCTAAATTGCGTGGTAGCTGCAGACTATTTTGTAAGTAATGGCAAACTGACAGCTACACGATATCAAGCCGTCGCGTATCAGGT‑GAACAAACAGAAAAAAAGACTGCCAAAATTTTCTCCAACAGCAGAAt  >  1:454251/1‑271 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                           
TTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATACCAACGTTTGGCGATATCAAAGCTAATAAGGCGTTCAACTTTTCCTGAAACAGTGCTGATGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGT‑GAACAAAACGCAAAATCGTCTGCCAAATTTTTCTCCAGCAGCAGAAT  >  NC_000913/3663994‑3664531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑