Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 263,603 G→A 18.8% Q23* (CAA→TAA)  ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913263,6030GA18.8% 47.9 / 2.4 21Q23* (CAA→TAA) ykfICP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (8/9);  new base A (2/2);  total (10/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGC  >  NC_000913/263438‑263791
                                                                                                                                                                     |                                                                                                                                                                                            
tCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACg                                                                                                                                                                                 >  1:159303/1‑179 (MQ=255)
gcgtatCGGTATGAACCGGCTGGTGTTTTTTTACCGTAAATTTCACGTGGTCGGCTTGAGTTGTACCGGCATCATGGTGTTCCTGAATCCCGGCCTCATTGCAGAATGGCGTGTCGTTTATGGTCATACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACTGGTAACAGGCAGTGCTTCACCGCCCGCTGAGTTATTGCACGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGTGGTAAATgg                                                                                 <  2:37086/270‑1 (MQ=21)
    gTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTg                                                                                                                                                                             <  2:159303/179‑1 (MQ=255)
                                                              aTACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGAcaggc                                                                                                                                                                       >  1:157055/1‑127 (MQ=255)
                                                                  cGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAggg                                                                                                                                                                   <  2:157055/127‑1 (MQ=255)
                                                                    gCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTg                                                                                                                                                                                              <  2:86694/98‑1 (MQ=255)
                                                                             tgtTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTACCAGACAGCCAc                                                                                                                                                                                  >  2:14295/1‑101 (MQ=255)
                                                                                 ccTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTACCAGACAGCCACGGGt                                                                                                                                                                              <  1:14295/101‑1 (MQ=255)
                                                                                    tAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGa                                                                                                                                                                            >  1:6606/1‑100 (MQ=255)
                                                                                        cacaGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGAcagg                                                                                                                                                                        <  2:6606/100‑1 (MQ=255)
                                                                                                                                           ttCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGc                                                                  >  1:257500/1‑151 (MQ=255)
                                                                                                                                           ttCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGtt                                  >  1:242501/1‑183 (MQ=255)
                                                                                                                                               agcagACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCa                                                              <  2:257500/151‑1 (MQ=255)
                                                                                                                                               agcagACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAAt                              <  2:242501/183‑1 (MQ=255)
                                                                                                                                                gcagACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTaa                                                                                     >  1:261063/1‑127 (MQ=255)
                                                                                                                                                gcagACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGGTTTCATGTTTGCTCTCGTGTAGGTAATTaa                                                                                                    >  1:290454/1‑112 (MQ=255)
                                                                                                                                                gcagACGTGTCAGTAACATTTACCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGt      >  1:109103/1‑206 (MQ=255)
                                                                                                                                                    aCGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCt                                                                                                <  2:290454/112‑1 (MQ=255)
                                                                                                                                                    aCGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATgg                                                                                 <  2:261063/127‑1 (MQ=255)
                                                                                                                                                    aCGTGTCAGTAACATTTACCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGc  <  2:109103/206‑1 (MQ=255)
                                                                                                                                                     cGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTtgt                        >  1:318185/1‑183 (MQ=255)
                                                                                                                                                         tCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTa                    <  2:318185/183‑1 (MQ=255)
                                                                                                                                                                     |                                                                                                                                                                                            
TCCTGTCGATACGAACCAGCTCGTATTTTTCTACCAGAAAATTCACGGCATCGGCTAGGGTGATACCGGCATCGATGTGTTCCTTAATCACAGCCTCATTGCAGAATGGCGTGTCGTTTATTGTCAGACCATAGTGCTGTTCCAGCAGACGTGTCAGTAACATTTGCCAGACAGCCACGGGTGACAGGCAGGGCTTCACCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGC  >  NC_000913/263438‑263791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: