Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	819,360	G→T	24.0%	L22L (CTG→CTT)	moaE →	molybdopterin synthase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	819,360	0	G	T	24.0%	36.2 / 4.6	16	L22L (CTG→CTT)	moaE	molybdopterin synthase catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base T (2/2);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCACATGGCTGCGCAGAGCGATCGCTGGGCGCTGGCGCTGGAAGATGGCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAACCATAACCTGGGCGACAGCGTCA  >  NC_000913/819144‑819436                                                                                                                                                                                                                         |                                                                             cAGCACATGGCTGCGCAGAGCGATCGCTGGGCGCTGGCGCTGGAAGATGGCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGa                                                             <  1:31732/234‑1 (MQ=255)                       aTCGCTGGGCGCTGGCGCTGGAAGATGGCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTgcg                            <  1:309325/245‑1 (MQ=255)                                                                                                 ccGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTTGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAACCATAACCTGGGCGACAGc      >  2:178478/1‑193 (MQ=255)                                                                                                 ccGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAgg                                >  2:148131/1‑167 (MQ=255)                                                                                                     tgactgacGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTTGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAACCATAACCTGGGCGACAGCGTCa  <  1:178478/193‑1 (MQ=255)                                                                                                     tgactgacGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTgcg                            <  1:148131/167‑1 (MQ=255)                                                                                                            cGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCa                                               >  2:260129/1‑141 (MQ=255)                                                                                                                gacgaAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCtt                                           <  1:260129/141‑1 (MQ=255)                                                                                                                                               gAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAACCATAACCTGGGCGACAGc      >  1:140167/1‑147 (MQ=255)                                                                                                                                                   ttAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAACCATAACCTGGGCGACAGCGTCa  <  2:140167/147‑1 (MQ=255)                                                                                                                                                          ggCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACg                                                                 >  1:62358/1‑77 (MQ=255)                                                                                                                                                           gCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTTGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTAc                                        >  2:169799/1‑101 (MQ=255)                                                                                                                                                              gAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGa                                                             <  2:62358/77‑1 (MQ=255)                                                                                                                                                               aaaCCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTTGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGt                                    <  1:169799/101‑1 (MQ=255)                                                                                                                                                                             gttgGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTgcgc                           >  1:345941/1‑96 (MQ=255)                                                                                                                                                                                 gTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAAcc                       <  2:345941/96‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                             CAGCACATGGCTGCGCAGAGCGATCGCTGGGCGCTGGCGCTGGAAGATGGCAAATTACTGGCTGCCGTCAACCAGACGCTGGTGAGTTTTGACCATCCGCTGACTGACGGCGACGAAGTAGCTTTCTTCCCGCCGGTAACCGGAGGTTAAGATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCGTGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAACCATAACCTGGGCGACAGCGTCA  >  NC_000913/819144‑819436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑