| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,994,744:1 | +A | 15.4% | coding (616/657 nt) | uvrY ← | DNA‑binding transcriptional activator UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,994,744 | 1 | . | A | 15.4% | 72.4 / 16.3 | 26 | coding (616/657 nt) | uvrY | DNA‑binding transcriptional activator UvrY |
| Reads supporting (aligned to +/- strand): ref base . (10/12); new base A (2/2); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01 | |||||||||||
GTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGC > NC_000913/1994501‑1994994 | gTTACATTAATTTGCTGGATCTGGGCTACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGc < 1:232130/273‑1 (MQ=255) aGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACttactgtccctt > 1:264080/1‑262 (MQ=255) cgcTTCGGTTTTGCGCGAAGCGAGGTCGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCCCTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AAATTCCCGGTGAGTTAGCGCAACACCCCCAAGAAAGTTTCGACTACTTAAAATAccaaag > 2:233119/1‑268 (MQ=255) tggtttttttttACGGAAATGGTTGGAAGCCCGTTTTTTCAGGTCTGTCGCTTTGCCGACATAGATAACCGTACCCCCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGt < 2:294357/261‑1 (MQ=255) aGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCg < 1:94187/273‑1 (MQ=255) ttttttCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCa < 1:233119/273‑1 (MQ=255) cGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATgtttagt > 2:203974/1‑181 (MQ=255) cGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATgtttagt < 1:203974/181‑1 (MQ=255) ccGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGAc < 1:187335/243‑1 (MQ=255) tACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCaa < 2:44071/146‑1 (MQ=255) tACCACAAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCaa > 1:44071/1‑146 (MQ=255) caGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAAtt > 1:293393/1‑272 (MQ=255) tCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGAtt > 2:122153/1‑206 (MQ=255) tCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGAtt > 2:122152/1‑206 (MQ=255) tCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGAtt < 1:122153/206‑1 (MQ=255) tCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGAtt < 1:122152/206‑1 (MQ=255) aTAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTg < 1:94461/231‑1 (MQ=255) aaCGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCg < 1:108880/181‑1 (MQ=255) aaCGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCg > 2:108880/1‑181 (MQ=255) gTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTTat > 2:94655/1‑217 (MQ=255) gTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGat < 1:94655/217‑1 (MQ=255) ttGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGatcatc < 2:279106/173‑1 (MQ=255) ttGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGatcatc > 1:279106/1‑173 (MQ=255) aaTGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTACCGCCATTTGTTGAGCGATGTCAg > 1:4837/1‑265 (MQ=255) aTGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACAt < 2:180016/60‑1 (MQ=255) aTGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACAt > 1:180016/1‑60 (MQ=255) g‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGc < 1:307903/251‑1 (MQ=255) | GTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCG‑AATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCATCAGCATAATCTGCAATTCACGTTCAGACAAACTGGCAAATGGGCTTTCTGTTTTTTCTGGTTCGATCTGGCTTAACGCCATTTGTTGAGCGATGTCAGAAGCAATGTAACGC > NC_000913/1994501‑1994994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |