Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,076,773	C→A	8.5%	A704S (GCT→TCT)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,076,773	0	C	A	8.5%	160.7 / 5.0	47	A704S (GCT→TCT)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (24/19);  new base A (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

ACAGGCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCG  >  NC_000913/1076568‑1077035                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                         acaGGCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCAcg                                                                                                                                                                                                       >  1:91397/1‑272 (MQ=255) acaGGCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCAc                                                                                                                                                                                                        >  1:91714/1‑271 (MQ=255) acaGGCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCCGGTTATTAACCGC‑CAGTTCCACCCCCCGTTccccctccag                                                                                                                                                                                                       >  2:73668/1‑263 (MQ=255)   aGGCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAAc                                                                                                                                                                                                                                      >  1:379963/1‑240 (MQ=255)   aGGCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCCCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAACCCAGATTGGCGCGTTATTAACCCC‑ACTTTCCCGCCCCTTGTCttcttctttct                                                                                                                                                                                                     >  2:49284/1‑267 (MQ=255)    ggCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAAcc                                                                                                                                                                                                                                     <  2:379963/240‑1 (MQ=255)    ggCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAg                                                                                                                                                                                                                         <  1:49284/251‑1 (MQ=255)     gCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCCGATTGGCGCGTTATTAACCGCAACTCTCC‑GCCCCTGTTCCACCTccccgcg                                                                                                                                                                                                    >  2:301787/1‑265 (MQ=255)     gCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACgcgg                                                                                                                                                                                                    >  1:255937/1‑271 (MQ=255)     gCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCACCACGGCACCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTccc                                                                                                                                                                                                                          >  1:400866/1‑248 (MQ=255)      cccTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGTGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACgcggcg                                                                                                                                                                                                  >  1:147348/1‑272 (MQ=255)      cccTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACGGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCAC‑ACTTTACAGCGCCGGTTCTACTTCACgcggcg                                                                                                                                                                                                  >  1:292583/1‑272 (MQ=255)                    gggtgtgcttAGCGGAAACCATCCCGACCCTGTCCGGCGTAGTAGTGGGGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGTGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGcac                                                                                                                                                                                     <  2:147348/264‑1 (MQ=255)                      gTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCa                                                                                                                                                                                                                          >  1:128224/1‑232 (MQ=255)                       tttCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAg                                                                                                                                                                                                                         <  2:128224/232‑1 (MQ=255)                                                                           cGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑CCTTTCCAGCGCCTGTTCTACTTCCCGCGGCGTGGCCTCCCGCCCCTCGCCCCACATACCCTTCCGTTTCCGC‑GGGTTAAAAACGAGcccacaccgcacc                                                                                                                             >  2:390279/1‑260 (MQ=255)                                                                                              ggCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGACGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCa                                                                                                                                                                                                                          >  2:84879/1‑161 (MQ=255)                                                                                               gCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGACGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAg                                                                                                                                                                                                                         <  1:84879/161‑1 (MQ=255)                                                                                                                     ttCCGGCCTCACGCACCAGAATACCAATCAGTTTCTGCATCTGGCTTTCCATCAGCACGGTAGCGCTGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAAACAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCTGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCAttt                                                                                    <  1:73668/271‑1 (MQ=255)                                                                                                                       ccGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCAttttt                                                                                  <  1:144526/271‑1 (MQ=255)                                                                                                                        cGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCa                                                                                                                                                                                                                          >  2:374086/1‑135 (MQ=255)                                                                                                                         ggCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAg                                                                                                                                                                                                                         <  1:374086/135‑1 (MQ=255)                                                                                                                         ggCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCa                                                                               <  1:398510/272‑1 (MQ=255)                                                                                                                                   aCCAGAATACCAATCTGTTTCTGCTTCTGGCTTTCCATCAGCACGGCAGCGCGGTGCACAAGCGCTGCGCGTTCATCGGGAGGCGTGGCAAACCAGATTGGCGCTTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGTTTAAGACCGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTAtt                                                                     <  1:387632/272‑1 (MQ=255)                                                                                                                                                  aGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTc                                                                                                                                                            >  1:6473/1‑170 (MQ=255)                                                                                                                                                  aGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGAACTAt                                                                      >  1:217182/1‑256 (MQ=255)                                                                                                                                                  aGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGACGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGtt                                                                                                                                                             >  1:336539/1‑169 (MQ=255)                                                                                                                                                   gTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGACGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTc                                                                                                                                                            <  2:336539/169‑1 (MQ=255)                                                                                                                                                                        tCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACgcggcg                                                                                                                                                                                                  >  1:329760/1‑111 (MQ=255)                                                                                                                                                                         cAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGt                                                                                                                                                                                                 <  2:329760/111‑1 (MQ=255)                                                                                                                                                                         cAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGt                               <  1:301787/272‑1 (MQ=255)                                                                                                                                                                           cagcGGCAGCGGGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGAGTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGtt                              <  1:290618/268‑1 (MQ=255)                                                                                                                                                                           caccgatggcgcGGTGACAAATCGCGGGGCGTTCAGCCGGTGGCGTGCCATACCAGGTTGGCGCGTTATTAACCGC‑ACTTTCCGGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTa                             <  2:255937/264‑1 (MQ=255)                                                                                                                                                                               ggCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAAc                                                                                                                                             >  2:390831/1‑156 (MQ=255)                                                                                                                                                                                gCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACg                                                                                                                                            <  1:390831/156‑1 (MQ=255)                                                                                                                                                                                 cAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGc                                                                                >  2:441477/1‑215 (MQ=255)                                                                                                                                                                                  aGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCa                                                                               <  1:441477/215‑1 (MQ=255)                                                                                                                                                                                       ggTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCa                                                                                                                                                                                                                          >  2:365539/1‑72 (MQ=255)                                                                                                                                                                                       ggTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGtt                                                                                                                                                             >  1:376193/1‑132 (MQ=255)                                                                                                                                                                                        gTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAg                                                                                                                                                                                                                         <  1:365539/72‑1 (MQ=255)                                                                                                                                                                                        gTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTc                                                                                                                                                            <  2:376193/132‑1 (MQ=255)                                                                                                                                                                                            aaaaTCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCt                                                                                                                       >  1:52989/1‑165 (MQ=255)                                                                                                                                                                                            aaaaTCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGGGATccgggcccg             >  2:69693/1‑264 (MQ=255)                                                                                                                                                                                             aaaTCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGcc                                            <  1:50650/239‑1 (MQ=255)                                                                                                                                                                                                      cgcgTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCCTTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAAAGCGAGGCCCGGCGGTGTTCGTTATCAAGAACCAGGCCCGGCGAGATGttg  >  2:379288/1‑270 (MQ=255)                                                                                                                                                                                                      cgcgTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGc                                             >  2:443035/1‑229 (MQ=255)                                                                                                                                                                                                       gcgTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGcc                                            <  1:443035/229‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                         ACAGGCCCTAATGGACGGTGGGTTTCGTTAGC‑GAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGCGTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGC‑ACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAG‑CCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCG  >  NC_000913/1076568‑1077035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑