Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,330 (G)8→7 noncoding (72/87 nt) leuQ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,3230G.99.4% 761.2 / ‑2.4 153noncoding (79/87 nt)leuQtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (94/58);  total (95/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

CCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGA  >  NC_000913/4606223‑4606423
                                                                                                    |                                                                                                    
cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGGAATATCAACGTGGTTTTTGGTGCGAg                                                                                                      <  1:6839709/101‑1 (MQ=255)
cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAg                                                                                                      <  2:6839810/101‑1 (MQ=255)
cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAg                                                                                                      <  2:6839897/101‑1 (MQ=255)
 ccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgg                                                                                                     >  2:6840242/1‑101 (MQ=255)
 ccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgg                                                                                                     >  1:6840241/1‑101 (MQ=255)
  cccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggg                                                                                                    >  1:6840244/1‑101 (MQ=255)
    cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                                                                  <  2:6839521/101‑1 (MQ=255)
    cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                                                                  >  2:6840247/1‑101 (MQ=255)
    cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                                                                  >  2:6840249/1‑101 (MQ=255)
     aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                                                                 <  1:6839548/101‑1 (MQ=255)
     aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                                                                 <  2:6839757/101‑1 (MQ=255)
      cGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                                                                                                >  1:6840251/1‑101 (MQ=255)
       gTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggga                                                                                               <  1:6839728/101‑2 (MQ=255)
         ccGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggact                                                                                             >  1:6840255/1‑98 (MQ=255)
         ccGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                                                                                                >  2:6840256/1‑98 (MQ=255)
          cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggactt                                                                                            <  2:6839642/101‑5 (MQ=255)
          cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggactt                                                                                            <  1:6839584/101‑5 (MQ=255)
           gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTg                                                                                          <  2:6839935/101‑1 (MQ=255)
           gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTg                                                                                          >  2:6840259/1‑101 (MQ=255)
            tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGa                                                                                         >  2:6840261/1‑101 (MQ=255)
            tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGa                                                                                         <  1:6839644/101‑1 (MQ=255)
             aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGaa                                                                                        <  1:6839695/101‑1 (MQ=255)
               gAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAAcc                                                                                      >  2:6840263/1‑101 (MQ=255)
                  cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAAccccc                                                                                   >  1:6840266/1‑101 (MQ=255)
                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCa                                                                                  >  1:6840268/1‑101 (MQ=255)
                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCa                                                                                  >  2:6840269/1‑101 (MQ=255)
                    cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCAc                                                                                 <  2:6839766/101‑1 (MQ=255)
                      aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGt                                                                               <  2:6839830/101‑1 (MQ=255)
                        cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                             >  2:6840275/1‑101 (MQ=255)
                        cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                             <  1:6839953/101‑1 (MQ=255)
                        cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                             <  1:6839755/101‑1 (MQ=255)
                        cacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                             >  1:6840274/1‑101 (MQ=255)
                            tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTaa                                                                         >  1:6840278/1‑101 (MQ=255)
                              aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                       >  2:6840280/1‑101 (MQ=255)
                               aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                             >  1:6840282/1‑94 (MQ=255)
                                gCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                     >  2:6840284/1‑101 (MQ=255)
                                   aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                  >  1:6840288/1‑101 (MQ=255)
                                   aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                  <  1:6840232/101‑1 (MQ=255)
                                      gcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                                               >  2:6840290/1‑101 (MQ=255)
                                         tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                            >  1:6840293/1‑101 (MQ=255)
                                         tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                            >  2:6840294/1‑101 (MQ=255)
                                          cTACCAATTCCGCCACCTTCGCCCAGGCCGAGCAATATCAACGTGGGTTTTGGTGCGAggg                                                                                                    >  2:6840296/1‑61 (MQ=37)
                                           tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                          >  2:6840298/1‑101 (MQ=255)
                                             ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGaa                                                        <  1:6839841/101‑1 (MQ=255)
                                             ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa                                                        >  2:6840301/1‑101 (MQ=255)
                                              cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggg                                                                                               >  2:6840304/1‑62 (MQ=255)
                                              cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                       >  1:6840303/1‑101 (MQ=255)
                                                aTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                     <  1:6839926/101‑1 (MQ=255)
                                                aTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTAAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                     >  2:6840307/1‑101 (MQ=255)
                                                 ttCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                    <  1:6839803/101‑1 (MQ=255)
                                                     gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                >  1:6840309/1‑101 (MQ=255)
                                                     gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                <  1:6839826/101‑1 (MQ=255)
                                                       cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                             <  2:6839702/70‑1 (MQ=255)
                                                       cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                              >  2:6840311/1‑101 (MQ=255)
                                                         ccTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAAc                                                                                       <  1:6839463/58‑1 (MQ=255)
                                                           ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                          >  1:6840314/1‑101 (MQ=255)
                                                             cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                                                                                                >  1:6840317/1‑46 (MQ=255)
                                                             cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                        >  2:6840316/1‑101 (MQ=255)
                                                               cccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACg                                                                                <  2:6839914/59‑1 (MQ=255)
                                                               cccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                      >  1:6840320/1‑101 (MQ=255)
                                                                ccAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                     <  2:6839866/101‑1 (MQ=255)
                                                                  aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                 <  1:6840087/87‑1 (MQ=255)
                                                                  aGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                   >  2:6840322/1‑101 (MQ=255)
                                                                   gTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc                                  >  1:6840323/1‑100 (MQ=255)
                                                                     gCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                                       <  2:6839594/78‑1 (MQ=255)
                                                                      cGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                               >  2:6840326/1‑101 (MQ=255)
                                                                       gAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                <  1:6839780/67‑1 (MQ=255)
                                                                       gAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                              >  1:6840327/1‑101 (MQ=255)
                                                                       gAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                              <  1:6839778/101‑1 (MQ=255)
                                                                       gAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                              >  2:6840330/1‑101 (MQ=255)
                                                                        aGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg                                                          <  1:6839882/72‑1 (MQ=255)
                                                                        aGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                             >  1:6840331/1‑101 (MQ=255)
                                                                         gCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                            >  1:6840333/1‑101 (MQ=255)
                                                                           aaTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                          <  2:6839838/85‑1 (MQ=255)
                                                                           aaTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                          <  2:6840100/101‑1 (MQ=255)
                                                                           aaTATCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                          >  1:6840336/1‑101 (MQ=255)
                                                                            atatCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                         >  1:6840338/1‑101 (MQ=255)
                                                                              atCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                             <  1:6839846/47‑1 (MQ=18)
                                                                              atCAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                       >  2:6840340/1‑101 (MQ=255)
                                                                                cAACGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa                     >  1:6840341/1‑101 (MQ=255)
                                                                                 aaCGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAc                    >  2:6840345/1‑101 (MQ=255)
                                                                                 aaCGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAc                    >  1:6840343/1‑101 (MQ=255)
                                                                                  aCGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACGCCTGa                                                         <  2:6840002/63‑1 (MQ=14)
                                                                                  aCGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                          <  1:6839750/94‑1 (MQ=255)
                                                                                   cGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACCCt                                                                  >  1:6840350/1‑53 (MQ=17)
                                                                                   cGTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCa                  >  2:6840348/1‑101 (MQ=255)
                                                                                    gTGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                 >  1:6840352/1‑101 (MQ=255)
                                                                                     tGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATc                >  1:6840354/1‑101 (MQ=255)
                                                                                     tGGTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATc                >  2:6840356/1‑101 (MQ=255)
                                                                                      ggTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCa               <  1:6840205/101‑1 (MQ=255)
                                                                                      ggTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCa               <  1:6839855/101‑1 (MQ=255)
                                                                                       gTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa              >  1:6840358/1‑101 (MQ=255)
                                                                                       gTTTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa              >  2:6840359/1‑101 (MQ=255)
                                                                                        tatttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                            <  1:6839745/68‑1 (MQ=11)
                                                                                         ttttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                      <  1:6839253/91‑1 (MQ=16)
                                                                                         ttttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt            >  1:6840361/1‑101 (MQ=255)
                                                                                              gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa       >  2:6840364/1‑101 (MQ=255)
                                                                                                 gcgggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa                     <  2:6839719/82‑1 (MQ=14)
                                                                                                   gggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                 <  1:6839878/86‑1 (MQ=37)
                                                                                                    ggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa  <  2:6839946/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGA  >  NC_000913/4606223‑4606423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑