BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,564,437	G→A	R85W (CGG→TGG)	yjiL ←	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,564,437	0	G	A	100.0%	1269.1 / NA	294	R85W (CGG→TGG)	yjiL	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (149/145); total (149/145)

Only 100 of 294 total aligned reads displayed.

CAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGT  >  NC_000913/4564339‑4564533

caGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATg                                                                                                >  1:6774668/1‑101 (MQ=255)

caGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATg                                                                                                <  2:6774277/101‑1 (MQ=255)

  ggAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATgcg                                                                                              >  2:6774672/1‑101 (MQ=255)

   gAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATgcgc                                                                                             <  1:6774469/101‑1 (MQ=255)

   gAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATgcgc                                                                                             <  2:6774258/101‑1 (MQ=255)

    aaaTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGcc                                                                                            >  1:6774676/1‑101 (MQ=255)

      aTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCa                                                                                          <  2:6774345/101‑1 (MQ=255)

      aTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCa                                                                                          <  1:6774364/101‑1 (MQ=255)

       tCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAg                                                                                         >  1:6774681/1‑101 (MQ=255)

          cacaGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGccc                                                                                      <  2:6774292/101‑1 (MQ=255)

           acaGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATgc                                                                                               >  2:6774686/1‑91 (MQ=255)

           acaGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCg                                                                                     <  2:6774164/101‑1 (MQ=255)

           acaGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCg                                                                                     >  1:6774685/1‑101 (MQ=255)

             aGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTg                                                                                   <  2:6774145/101‑1 (MQ=255)

             aGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTg                                                                                   <  1:6774205/101‑1 (MQ=255)

             aGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTg                                                                                   >  2:6774691/1‑101 (MQ=255)

               gTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGAc                                                                                 >  1:6774694/1‑101 (MQ=255)

               gTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCCCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGAc                                                                                 <  1:6774125/101‑1 (MQ=255)

                ttACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACa                                                                                >  2:6774695/1‑101 (MQ=255)

                ttACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACa                                                                                <  2:6774498/101‑1 (MQ=255)

                    cGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGgag                                                                            <  1:6774275/101‑1 (MQ=255)

                    cGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGgag                                                                            >  2:6774700/1‑101 (MQ=255)

                     gTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGgaga                                                                           >  1:6774701/1‑101 (MQ=255)

                      tcatcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGAt                                                                          >  1:6774705/1‑101 (MQ=255)

                       catcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGAtt                                                                         >  1:6774709/1‑101 (MQ=255)

                       catcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGAtt                                                                         <  1:6774373/101‑1 (MQ=255)

                       catcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCAGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGAtt                                                                         >  2:6774710/1‑101 (MQ=255)

                        atcatcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGAttt                                                                        <  2:6774317/101‑1 (MQ=255)

                          catcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTcc                                                                      <  1:6774356/101‑1 (MQ=255)

                          catcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTcc                                                                      <  1:6774396/101‑1 (MQ=255)

                          catcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTcc                                                                      <  1:6774035/101‑1 (MQ=255)

                           atcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCg                                                                     >  2:6774715/1‑101 (MQ=255)

                            tcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGt                                                                    <  2:6774379/101‑1 (MQ=255)

                            tcaAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGt                                                                    >  1:6774716/1‑101 (MQ=255)

                              aaGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTa                                                                  <  2:6773978/101‑1 (MQ=255)

                                     aTCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGttt                                                           >  1:6774721/1‑101 (MQ=255)

                                     aTCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGttt                                                           >  2:6774722/1‑101 (MQ=255)

                                     aTCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGttt                                                           <  2:6774265/101‑1 (MQ=255)

                                      tCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTa                                                          <  1:6774308/101‑1 (MQ=255)

                                       cACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTAt                                                         <  1:6774218/101‑1 (MQ=255)

                                       cACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTAt                                                         >  2:6774727/1‑101 (MQ=255)

                                         cTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCg                                                       >  1:6774728/1‑101 (MQ=255)

                                          tttGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCgg                                                      >  1:6774732/1‑101 (MQ=255)

                                           ttGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGc                                                     <  1:6774242/101‑1 (MQ=255)

                                            tGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCa                                                    >  1:6774735/1‑101 (MQ=255)

                                                 tCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATc                                               <  2:6774386/101‑1 (MQ=255)

                                                 tCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATc                                               >  2:6774740/1‑101 (MQ=255)

                                                 tCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTCATCGGCAAAATc                                               >  1:6774741/1‑101 (MQ=255)

                                                   cTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATcca                                             >  2:6774744/1‑101 (MQ=255)

                                                   cTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGAAAGGAGATTTCCGTTACCTGTTTATCGGCAAAATcca                                             <  2:6774331/101‑1 (MQ=255)

                                                     gACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccacc                                           >  1:6774750/1‑101 (MQ=255)

                                                     gACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccacc                                           >  2:6774748/1‑101 (MQ=255)

                                                      accaccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATccacca                                          <  2:6774435/101‑1 (MQ=255)

                                                       ccaccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAg                                         >  1:6774754/1‑101 (MQ=255)

                                                        caccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGt                                        >  2:6774756/1‑101 (MQ=255)

                                                         accGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGtt                                       <  2:6774230/101‑1 (MQ=255)

                                                          ccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTg                                      <  2:6774420/101‑1 (MQ=255)

                                                          ccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTg                                      >  2:6774760/1‑101 (MQ=255)

                                                            gATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTgcc                                    <  2:6774607/101‑1 (MQ=255)

                                                            gATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTgcc                                    >  1:6774765/1‑101 (MQ=255)

                                                            gATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTgcc                                    >  2:6774763/1‑101 (MQ=255)

                                                              tGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTgccgc                                  <  1:6774456/101‑1 (MQ=255)

                                                                tCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGccc                                <  2:6774366/101‑1 (MQ=255)

                                                                tCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGccc                                <  1:6774171/101‑1 (MQ=255)

                                                                 cGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCg                               >  2:6774770/1‑101 (MQ=255)

                                                                 cGATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCg                               >  1:6774771/1‑101 (MQ=255)

                                                                  gATTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGt                              <  1:6774391/101‑1 (MQ=255)

                                                                   aTTACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTa                             >  2:6774776/1‑101 (MQ=255)

                                                                    ttACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAg                            >  1:6774777/1‑101 (MQ=255)

                                                                     tACCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGc                           <  1:6774190/101‑1 (MQ=255)

                                                                      aCCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGcc                          <  1:6774264/101‑1 (MQ=255)

                                                                      aCCGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGcc                          >  2:6774780/1‑101 (MQ=255)

                                                                       ccGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCg                         <  1:6774301/101‑1 (MQ=255)

                                                                       ccGCGCGCGTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCg                         <  2:6774309/101‑1 (MQ=255)

                                                                         gcgcgcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGt                       <  2:6774449/101‑1 (MQ=255)

                                                                         gcgcgcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGt                       >  1:6774787/1‑101 (MQ=255)

                                                                         gcgcgcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGt                       >  2:6774785/1‑101 (MQ=255)

                                                                          cgcgcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTg                      >  2:6774789/1‑101 (MQ=255)

                                                                          cgcgcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTg                      <  2:6774735/101‑1 (MQ=255)

                                                                           gcgcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGa                     <  1:6774383/101‑1 (MQ=255)

                                                                           gcgcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGa                     <  1:6774237/101‑1 (MQ=255)

                                                                             gcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGc                   <  2:6774112/101‑1 (MQ=255)

                                                                             gcgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGc                   <  1:6774546/101‑1 (MQ=255)

                                                                              cgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCg                  >  1:6774794/1‑101 (MQ=255)

                                                                              cgTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCg                  >  2:6774795/1‑101 (MQ=255)

                                                                               gTTGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGt                 <  2:6774181/101‑1 (MQ=255)

                                                                               gTTGCTGGAGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGtt                                       >  2:6774800/1‑79 (MQ=255)

                                                                                 tGCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCa               <  2:6774470/101‑1 (MQ=255)

                                                                                  gCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAg              <  2:6774340/101‑1 (MQ=255)

                                                                                  gCTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAg              <  2:6774395/101‑1 (MQ=255)

                                                                                   cTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGa             >  1:6774803/1‑101 (MQ=255)

                                                                                   cTGGCGCAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGa             <  2:6774238/101‑1 (MQ=255)

                                                                                       cgcAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACg         >  1:6774806/1‑101 (MQ=255)

                                                                                        gcAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACgg        <  2:6774408/101‑1 (MQ=255)

                                                                                         cAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGc       >  1:6774809/1‑101 (MQ=255)

                                                                                         cAAGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGc       >  2:6774811/1‑101 (MQ=255)

                                                                                          aaGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCg      <  1:6774419/101‑1 (MQ=255)

                                                                                          aaGAAACCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCg      >  2:6774815/1‑101 (MQ=255)

                                                                                             aaaCCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCggaa                                                    >  1:6774818/1‑50 (MQ=255)

                                                                                              aaCCATGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGt  <  2:6774530/101‑1 (MQ=255)

CAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGT  >  NC_000913/4564339‑4564533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`