BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	159,035	A→G	V31A (GTG→GCG)	pcnB ←	poly(A) polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	159,035	0	A	G	100.0%	1065.8 / NA	256	V31A (GTG→GCG)	pcnB	poly(A) polymerase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (152/104); total (152/104)

Only 100 of 256 total aligned reads displayed.

CCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACA  >  NC_000913/158938‑159132

 cagGTTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGt                                                                                               >  2:239845/1‑101 (MQ=255)

   ggCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCa                                                                                             >  2:239849/1‑101 (MQ=255)

      ttCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCt                                                                                          >  2:239851/1‑101 (MQ=255)

       tCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCtg                                                                                         <  1:239548/101‑1 (MQ=255)

        cGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCtgt                                                                                        <  1:239370/101‑1 (MQ=255)

        cGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCtgt                                                                                        >  2:239854/1‑101 (MQ=255)

         gTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCtgtg                                                                                       <  2:239333/101‑1 (MQ=255)

          tATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTgg                                                                                      <  2:239475/101‑1 (MQ=255)

          tATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTgg                                                                                      >  2:239856/1‑101 (MQ=255)

            tCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCtg                                                                                         >  1:239860/1‑96 (MQ=255)

            tCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGAc                                                                                    <  2:239609/101‑1 (MQ=255)

            tCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGAc                                                                                    <  1:239557/101‑1 (MQ=255)

             cccGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACg                                                                                   <  2:239418/101‑1 (MQ=255)

             cccGCTTTATTGAGCCTGTACATTACCTTCAGGGCACTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACg                                                                                   <  1:239510/101‑1 (MQ=255)

              ccGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACgg                                                                                  >  2:239862/1‑101 (MQ=255)

                 cTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCg                                                                               >  1:239866/1‑101 (MQ=255)

                  tttATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGa                                                                              >  2:239868/1‑101 (MQ=255)

                  tttATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGa                                                                              >  1:239869/1‑101 (MQ=255)

                   ttATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGAc                                                                             >  2:239873/1‑101 (MQ=255)

                     aTTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTg                                                                           <  1:239404/101‑1 (MQ=255)

                     aTTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTg                                                                           <  2:239397/101‑1 (MQ=255)

                     aTTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTg                                                                           >  1:239876/1‑101 (MQ=255)

                       tGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGcc                                                                         >  2:239880/1‑101 (MQ=255)

                        gAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCt                                                                        >  2:239882/1‑101 (MQ=255)

                         aGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTg                                                                       >  1:239883/1‑101 (MQ=255)

                          gCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGt                                                                      <  2:239454/101‑1 (MQ=255)

                           ccTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGtt                                                                     >  1:239887/1‑101 (MQ=255)

                              gTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAg                                                                  <  2:239717/101‑1 (MQ=255)

                               tACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGc                                                                 >  2:239891/1‑101 (MQ=255)

                                aCATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGcc                                                                >  2:239893/1‑101 (MQ=255)

                                aCATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGcc                                                                <  2:239472/101‑1 (MQ=255)

                                   ttACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCg                                                             <  1:239617/101‑1 (MQ=255)

                                   ttACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCg                                                             <  1:239438/101‑1 (MQ=255)

                                    tACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGc                                                            >  2:239896/1‑101 (MQ=255)

                                     aCCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCt                                                           <  1:239428/101‑1 (MQ=255)

                                     aCCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCt                                                           >  2:239899/1‑101 (MQ=255)

                                      ccTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGACTCGCtt                                                          >  1:239900/1‑101 (MQ=255)

                                       cTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCttt                                                         <  2:239760/101‑1 (MQ=255)

                                        ttCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTc                                                        <  1:239464/101‑1 (MQ=255)

                                        ttCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTc                                                        >  2:239903/1‑101 (MQ=255)

                                          cAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCt                                                      >  2:239907/1‑101 (MQ=255)

                                          cAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCt                                                      <  2:239487/101‑1 (MQ=255)

                                           aGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTc                                                     <  2:239539/101‑1 (MQ=255)

                                           aGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTc                                                     <  2:239434/101‑1 (MQ=255)

                                           aGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTc                                                     >  1:239909/1‑101 (MQ=255)

                                           aGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTc                                                     >  2:239912/1‑101 (MQ=255)

                                            gggCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTcg                                                    <  1:239819/101‑1 (MQ=255)

                                             ggCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTcgc                                                   >  2:239916/1‑101 (MQ=255)

                                              gCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTcgcg                                                  >  2:239918/1‑101 (MQ=255)

                                               cATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCgg                                                 <  2:239647/101‑1 (MQ=255)

                                               cATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCgg                                                 >  1:239921/1‑101 (MQ=255)

                                                aTTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGc                                                >  1:239924/1‑101 (MQ=255)

                                                aTTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGc                                                <  1:239757/101‑1 (MQ=255)

                                                 ttttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCt                                               <  1:239295/101‑1 (MQ=255)

                                                 ttttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCt                                               <  1:239584/101‑1 (MQ=255)

                                                  tttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCtt                                              <  1:239190/101‑1 (MQ=255)

                                                   ttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTa                                             >  1:239926/1‑101 (MQ=255)

                                                    tCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAg                                            >  2:239928/1‑101 (MQ=255)

                                                     cACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTcg                                                    >  1:239931/1‑92 (MQ=255)

                                                      aCTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCa                                          >  1:239934/1‑101 (MQ=255)

                                                      aCTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCa                                          >  2:239933/1‑101 (MQ=255)

                                                        tGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCAcc                                        >  1:239939/1‑101 (MQ=255)

                                                        tGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCAcc                                        >  2:239941/1‑101 (MQ=255)

                                                         gATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCt                                       <  1:239569/101‑1 (MQ=255)

                                                         gATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCt                                       >  1:239944/1‑101 (MQ=255)

                                                          atatCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCtt                                      <  2:239591/101‑1 (MQ=255)

                                                          atatCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCtt                                      >  2:239946/1‑101 (MQ=255)

                                                             tCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCg                                   >  2:239949/1‑101 (MQ=255)

                                                              cTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCgg                                  >  1:239950/1‑101 (MQ=255)

                                                              cTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCgg                                  <  1:239493/101‑1 (MQ=255)

                                                                ttGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCa                                >  2:239952/1‑101 (MQ=255)

                                                                 tGCGGGAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCaa                               <  1:239896/101‑1 (MQ=255)

                                                                    gggAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCaaaaa                            >  2:239957/1‑101 (MQ=255)

                                                                     ggAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAAt                           <  1:239634/101‑1 (MQ=255)

                                                                     ggAAATAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAAt                           <  2:239714/101‑1 (MQ=255)

                                                                        aaTAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAg                        >  1:239960/1‑101 (MQ=255)

                                                                        aaTAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAg                        >  1:239958/1‑101 (MQ=255)

                                                                         aTAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGc                       >  1:239964/1‑101 (MQ=255)

                                                                         aTAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGc                       <  2:239572/101‑1 (MQ=255)

                                                                          tAGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCg                      >  2:239966/1‑101 (MQ=255)

                                                                           aGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGa                     >  1:239969/1‑101 (MQ=255)

                                                                           aGCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGa                     >  2:239972/1‑101 (MQ=255)

                                                                            gCATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGAc                    >  1:239973/1‑101 (MQ=255)

                                                                             cATGCTGCTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACt                   >  1:239975/1‑101 (MQ=255)

                                                                               tgctgcTCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCg                 >  2:239980/1‑101 (MQ=255)

                                                                                gctgctCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCgg                >  1:239981/1‑101 (MQ=255)

                                                                                 ctgctCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCggg               >  2:239986/1‑101 (MQ=255)

                                                                                 ctgctCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCggg               >  1:239985/1‑101 (MQ=255)

                                                                                   gctCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTa             <  2:239666/101‑1 (MQ=255)

                                                                                    ctCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCaa                               <  1:239606/82‑1 (MQ=255)

                                                                                    ctCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTaa            >  2:239990/1‑101 (MQ=255)

                                                                                     tCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTaaa           >  1:239991/1‑101 (MQ=255)

                                                                                     tCACGCGGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTaaa           >  2:239993/1‑101 (MQ=255)

                                                                                        cgcgGGATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAAt        <  1:239862/101‑1 (MQ=255)

                                                                                           gggATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGc                                    <  2:239498/70‑1 (MQ=255)

                                                                                           gggATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGt     >  2:239996/1‑101 (MQ=255)

                                                                                             gATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTac   <  2:239728/101‑1 (MQ=255)

                                                                                             gATCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTac   <  2:239336/101‑1 (MQ=255)

                                                                                              aTCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCaaaa                             <  2:239550/74‑1 (MQ=255)

                                                                                              aTCGCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTaca  <  2:239448/101‑1 (MQ=255)

CCAGGCTTCGTATCCCGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTAGCGACTCGGGTAAAAATAGTACA  >  NC_000913/158938‑159132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`