BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,864,358	G→A	G170D (GGT→GAT)	yeaD →	putative aldose 1‑epimerase YeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,864,358	0	G	A	100.0%	1214.5 / NA	283	G170D (GGT→GAT)	yeaD	putative aldose 1‑epimerase YeaD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (156/127); total (156/127)

Only 100 of 287 total aligned reads displayed.

GTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACC  >  NC_000913/1864260‑1864455

gTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATg                                                                                                 <  2:2718077/101‑1 (MQ=255)

  tAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGgatgat                                                                                               >  2:2718439/1‑101 (MQ=255)

    gcgcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGatat                                                                                             >  1:2718443/1‑101 (MQ=255)

     cgcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATc                                                                                            >  2:2718446/1‑101 (MQ=255)

     cgcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATc                                                                                            >  2:2718444/1‑101 (MQ=255)

      gcATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCg                                                                                           <  1:2718051/101‑1 (MQ=255)

       cATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGc                                                                                          >  2:2718452/1‑101 (MQ=255)

        aTTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCt                                                                                         >  1:2718454/1‑101 (MQ=255)

         tttCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTa                                                                                        >  2:2718456/1‑101 (MQ=255)

             cgcgTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAgg                                                                                     >  2:2718461/1‑100 (MQ=255)

              gcgTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTa                                                                                   >  1:2718463/1‑101 (MQ=255)

               cgTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTaa                                                                                  <  1:2718115/101‑1 (MQ=255)

                gTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGc                                                                                          <  2:2718274/92‑1 (MQ=255)

                gTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAg                                                                                 >  1:2718468/1‑101 (MQ=255)

                 tGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGc                                                                                <  1:2718362/101‑1 (MQ=255)

                  gggTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCg                                                                               >  2:2718471/1‑101 (MQ=255)

                   ggTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGt                                                                              <  1:2717860/101‑1 (MQ=255)

                    gTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTc                                                                             >  1:2718474/1‑101 (MQ=255)

                    gTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTc                                                                             <  1:2717952/101‑1 (MQ=255)

                     tAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCa                                                                            >  2:2718476/1‑101 (MQ=255)

                      aaaaCTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAg                                                                           >  1:2718481/1‑101 (MQ=255)

                      aaaaCTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAg                                                                           >  1:2718478/1‑101 (MQ=255)

                      aaaaCTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAg                                                                           <  1:2717807/101‑1 (MQ=255)

                      aaaaCTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAg                                                                           <  1:2718090/101‑1 (MQ=255)

                        aaCTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtg                                                                         >  2:2718484/1‑101 (MQ=255)

                           ttGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggc                                                                      >  1:2718487/1‑101 (MQ=255)

                            tgtgAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggct                                                                     <  2:2718045/101‑1 (MQ=255)

                             gtgAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggctg                                                                    >  1:2718491/1‑101 (MQ=255)

                             gtgAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggctg                                                                    <  2:2718245/101‑1 (MQ=255)

                              tgAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggctgg                                                                   >  2:2718493/1‑101 (MQ=255)

                              tgAAATCGATCTTGAAGCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggctgg                                                                   <  2:2718002/101‑1 (MQ=255)

                                aaaTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggctgggc                                                                 <  2:2717980/101‑1 (MQ=255)

                                aaaTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggctgggc                                                                 <  1:2718040/101‑1 (MQ=255)

                                aaaTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGtgggctgggc                                                                 <  2:2718225/101‑1 (MQ=255)

                                 aaTCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCg                                                                >  1:2718495/1‑101 (MQ=255)

                                     gATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCg                                                            >  1:2718503/1‑101 (MQ=255)

                                     gATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCg                                                            >  2:2718501/1‑101 (MQ=255)

                                     gATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCg                                                            >  1:2718499/1‑101 (MQ=255)

                                     gATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCg                                                            <  1:2718126/101‑1 (MQ=255)

                                      aTCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGc                                                           <  1:2718071/101‑1 (MQ=255)

                                      aTCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGc                                                           >  2:2718507/1‑101 (MQ=255)

                                        cTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCtt                                                         >  1:2718511/1‑101 (MQ=255)

                                        cTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCtt                                                         >  2:2718513/1‑101 (MQ=255)

                                         ttGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTc                                                        >  1:2718514/1‑101 (MQ=255)

                                          tGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCa                                                       >  1:2718518/1‑101 (MQ=255)

                                            aaTCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCAtt                                                     >  2:2718521/1‑101 (MQ=255)

                                            aaTCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCAtt                                                     <  1:2718163/101‑1 (MQ=255)

                                             aTCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTg                                                    >  1:2718523/1‑101 (MQ=255)

                                              tCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGa                                                   <  1:2718102/101‑1 (MQ=255)

                                               cacaTGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGAt                                                  >  1:2718527/1‑101 (MQ=255)

                                                 caTGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATaa                                                >  1:2718531/1‑101 (MQ=255)

                                                  aTGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATaaa                                               <  1:2718029/101‑1 (MQ=255)

                                                   tGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAg                                              <  2:2717951/101‑1 (MQ=255)

                                                    ggCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGt                                             <  2:2718111/101‑1 (MQ=255)

                                                    ggCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGt                                             <  1:2718269/101‑1 (MQ=255)

                                                     gCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTg                                            >  2:2718534/1‑101 (MQ=255)

                                                     gCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTg                                            <  2:2718084/101‑1 (MQ=255)

                                                      cGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGa                                           >  1:2718535/1‑101 (MQ=255)

                                                        aaTTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAGAGTGAAt                                         <  1:2718184/101‑1 (MQ=255)

                                                        aaTTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAAt                                         >  2:2718540/1‑101 (MQ=255)

                                                         aTTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTc                                                        >  1:2718542/1‑85 (MQ=255)

                                                          tttGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGa                                       <  2:2718323/101‑1 (MQ=255)

                                                           ttGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGAc                                      >  2:2718545/1‑101 (MQ=255)

                                                            tGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGAcg                                     >  1:2718548/1‑101 (MQ=255)

                                                              aaaCCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGAcgcg                                   <  2:2717858/101‑1 (MQ=255)

                                                              aaaCCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGAcgcg                                   >  1:2718552/1‑101 (MQ=255)

                                                              aaaCCACCTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGAcgcg                                   >  2:2718551/1‑101 (MQ=255)

                                                                accaccTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCgaa                                 >  2:2718556/1‑101 (MQ=255)

                                                                 ccaccTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCgaaa                                >  1:2718557/1‑101 (MQ=255)

                                                                 ccaccTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGACAAAGTGAATGACGCga                                  <  1:2718096/99‑1 (MQ=255)

                                                                  caccTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCgaaag                               <  1:2718022/101‑1 (MQ=255)

                                                                    ccTCTGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCgaaagaa                             >  2:2718562/1‑101 (MQ=255)

                                                                     ctctGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCgaaagaaa                            >  1:2718563/1‑101 (MQ=255)

                                                                     ctctGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCgaaagaaa                            <  2:2718154/101‑1 (MQ=255)

                                                                     ctctGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCgaaagaaa                            <  1:2718081/101‑1 (MQ=255)

                                                                      tctGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGaaaa                           >  2:2718566/1‑101 (MQ=255)

                                                                       ctGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAAt                          <  1:2717998/101‑1 (MQ=255)

                                                                       ctGCCCTGCATACCTACGTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAAt                          <  1:2718135/101‑1 (MQ=255)

                                                                        tGCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGTATGACGCGAAAGAAAATg                         <  1:2717786/101‑1 (MQ=255)

                                                                         gCCCTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGt                        <  1:2718219/101‑1 (MQ=255)

                                                                          cccTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTa                       <  1:2718322/101‑1 (MQ=255)

                                                                          cccTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTa                       <  2:2718351/101‑1 (MQ=255)

                                                                            cTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACt                     >  1:2718571/1‑101 (MQ=255)

                                                                            cTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACt                     <  1:2718255/101‑1 (MQ=255)

                                                                            cTGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACt                     <  1:2718171/101‑1 (MQ=255)

                                                                             tGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTg                    <  2:2718309/101‑1 (MQ=255)

                                                                             tGCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTg                    >  2:2718572/1‑101 (MQ=255)

                                                                              gCATACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGa                   <  2:2718129/101‑1 (MQ=255)

                                                                                aTACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGa                                                   >  2:2718577/1‑67 (MQ=255)

                                                                                 tACCTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCg                >  1:2718578/1‑101 (MQ=255)

                                                                                    cTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATg             <  2:2718199/101‑1 (MQ=255)

                                                                                    cTACTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATg             >  1:2718581/1‑101 (MQ=255)

                                                                                      aCTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGt           >  1:2718586/1‑101 (MQ=255)

                                                                                       cTTTAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTa          >  2:2718587/1‑101 (MQ=255)

                                                                                          tAACGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTc       <  2:2718207/101‑1 (MQ=255)

                                                                                           aaCGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCa      >  1:2718591/1‑101 (MQ=255)

                                                                                           aaCGTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCa      >  2:2718593/1‑101 (MQ=255)

                                                                                            aCGTGGATGATATCGCTAAGGTAAGCGTCAGtgggct                                                                     <  1:2717967/37‑1 (MQ=255)

                                                                                              gTGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGAc   <  2:2718186/101‑1 (MQ=255)

                                                                                               tGGATGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGAcc  >  1:2718597/1‑101 (MQ=255)

GTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCGCTAAGGTAAGCGTCAGTGGGCTGGGCGATCGCTTCATTGATAAAGTGAATGACGCGAAAGAAAATGTACTGACCGATGGTATTCAGACC  >  NC_000913/1864260‑1864455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`