Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,879,162 T→C T32A (ACT→GCT)  yeaQ ← PF04226 family protein YeaQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,879,1620TC96.3% 1074.3 / 19.2 273T32A (ACT→GCT) yeaQPF04226 family protein YeaQ
Reads supporting (aligned to +/- strand):  ref base T (7/3);  new base C (130/133);  total (137/136)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 273 total aligned reads displayed.

CACGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTT  >  NC_000913/1879064‑1879259
                                                                                                  |                                                                                                 
 aCGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCAt                                                                                                >  1:2740364/1‑101 (MQ=255)
   gAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATaa                                                                                              >  1:2740369/1‑101 (MQ=255)
   gAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATaa                                                                                              >  2:2740367/1‑101 (MQ=255)
    aaGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATaaa                                                                                             <  1:2739753/101‑1 (MQ=255)
    aaGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATaaa                                                                                             >  1:2740372/1‑101 (MQ=255)
     aGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAg                                                                                            <  2:2739963/101‑1 (MQ=255)
     aGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAg                                                                                            <  2:2739920/101‑1 (MQ=255)
      gCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGa                                                                                           >  2:2740375/1‑101 (MQ=255)
      gCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATaaa                                                                                             <  1:2739931/99‑1 (MQ=255)
       cTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGaa                                                                                          >  1:2740376/1‑101 (MQ=255)
           cAAAATTGAAGCCATCGCCTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATaa                                                                                              >  2:2740384/1‑93 (MQ=255)
           cAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCa                                                                                      >  1:2740380/1‑101 (MQ=255)
            aaaaTTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCAc                                                                                     >  1:2740385/1‑101 (MQ=255)
             aaaTTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCAcc                                                                                    <  1:2740057/101‑1 (MQ=255)
              aaTTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCt                                                                                   <  1:2739841/101‑1 (MQ=255)
               aTTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTc                                                                                  >  1:2740389/1‑101 (MQ=255)
               aTTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTc                                                                                  <  2:2739947/101‑1 (MQ=255)
                ttGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTcc                                                                                 >  1:2740391/1‑101 (MQ=255)
                 tGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCa                                                                                >  2:2740395/1‑101 (MQ=255)
                 tGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCa                                                                                >  1:2740396/1‑101 (MQ=255)
                 tGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCa                                                                                <  2:2739890/101‑1 (MQ=255)
                   aaGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATc                                                                              >  2:2740403/1‑101 (MQ=255)
                   aaGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATc                                                                              >  2:2740401/1‑101 (MQ=255)
                    aGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCt                                                                             >  1:2740404/1‑101 (MQ=255)
                     gCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCtt                                                                            <  1:2739976/101‑1 (MQ=255)
                      ccATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCttt                                                                           >  1:2740407/1‑101 (MQ=255)
                       cATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAAt                                                                                         >  1:2740409/1‑86 (MQ=255)
                           gACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTg                                                                      >  1:2740412/1‑101 (MQ=255)
                           gACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTAACCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTg                                                                      <  2:2740088/101‑1 (MQ=255)
                            aCTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTgg                                                                     <  1:2739925/101‑1 (MQ=255)
                             cTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGc                                                                    >  1:2740417/1‑101 (MQ=255)
                              tttACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCa                                                                   >  2:2740419/1‑101 (MQ=255)
                               ttACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCAt                                                                  <  2:2740024/101‑1 (MQ=255)
                                 aCCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGa                                                                <  2:2739584/101‑1 (MQ=255)
                                  ccAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGAt                                                               >  2:2740422/1‑101 (MQ=255)
                                     aaGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCa                                                            >  2:2740425/1‑101 (MQ=255)
                                       gCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACt                                                          >  1:2740428/1‑101 (MQ=255)
                                       gCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACt                                                          >  2:2740427/1‑101 (MQ=255)
                                       gCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACt                                                          <  1:2739961/101‑1 (MQ=255)
                                        ccAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACtt                                                         <  1:2739799/101‑1 (MQ=255)
                                         cAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTc                                                        <  1:2740121/101‑1 (MQ=255)
                                         cAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTc                                                        <  1:2739917/101‑1 (MQ=255)
                                          aaaCAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCg                                                       >  1:2740433/1‑101 (MQ=255)
                                            aCAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGcc                                                     <  1:2739898/101‑1 (MQ=255)
                                             cAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCa                                                    >  2:2740435/1‑101 (MQ=255)
                                                cGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGaa                                                 <  1:2740151/101‑1 (MQ=255)
                                                 gTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGa                                                  >  2:2740438/1‑99 (MQ=255)
                                                   gCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATAc                                              <  2:2739864/101‑1 (MQ=255)
                                                    cTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATAcc                                             <  2:2740020/101‑1 (MQ=255)
                                                     tGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATAcc                                             <  1:2740112/100‑1 (MQ=255)
                                                     tGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCg                                            >  2:2740440/1‑101 (MQ=255)
                                                      gATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCgg                                           >  1:2740444/1‑101 (MQ=255)
                                                       atccatccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGc                                          >  2:2740446/1‑101 (MQ=255)
                                                       atccatccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGc                                          <  1:2740258/101‑1 (MQ=255)
                                                        tccatccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCa                                         <  2:2740132/101‑1 (MQ=255)
                                                        tccatccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCa                                         <  1:2739989/101‑1 (MQ=255)
                                                          cacccgccgACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCgg                                           <  2:2739819/94‑1 (MQ=255)
                                                           atccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATa                                      >  2:2740451/1‑101 (MQ=255)
                                                           atccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATa                                      >  1:2740450/1‑101 (MQ=255)
                                                            tccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATaa                                     >  2:2740455/1‑101 (MQ=255)
                                                            tccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATaa                                     <  1:2740019/101‑1 (MQ=255)
                                                            tccGCCGACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATaa                                     <  1:2740074/101‑1 (MQ=255)
                                                             ccgccgACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAg                                    <  1:2739997/101‑1 (MQ=255)
                                                               gccgACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGcc                                                     >  2:2740457/1‑82 (MQ=255)
                                                                ccgACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCa                                                    <  2:2739972/82‑1 (MQ=255)
                                                                ccgACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGccc                                 <  2:2740063/101‑1 (MQ=255)
                                                                ccgACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGccc                                 <  1:2740042/101‑1 (MQ=255)
                                                                 cgACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCa                                >  1:2740458/1‑101 (MQ=255)
                                                                  gACTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCaa                               <  1:2739623/101‑1 (MQ=255)
                                                                   aCTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCaaa                              <  1:2740249/101‑1 (MQ=255)
                                                                    cTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCaaaa                             >  1:2740464/1‑101 (MQ=255)
                                                                    cTACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCACGATCCACTTCGCCAGAATACCGGCAATAAGCCCaaaa                             >  2:2740463/1‑101 (MQ=255)
                                                                     tACGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCaaaaa                            <  1:2739948/101‑1 (MQ=255)
                                                                       cGGCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAaata                          >  2:2740469/1‑101 (MQ=255)
                                                                        ggCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAaataa                         <  2:2740013/101‑1 (MQ=255)
                                                                         gCACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAaataat                        <  2:2740199/101‑1 (MQ=255)
                                                                          cACCGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATc                       <  1:2740004/101‑1 (MQ=255)
                                                                            ccGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCa                     <  1:2739885/101‑1 (MQ=255)
                                                                             cGACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCAt                    >  2:2740474/1‑101 (MQ=255)
                                                                              gACTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATg                   <  1:2740096/101‑1 (MQ=255)
                                                                               aCTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGa                  >  1:2740475/1‑101 (MQ=255)
                                                                               aCTATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGa                  <  2:2740193/101‑1 (MQ=255)
                                                                                 tATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACa                <  2:2739983/101‑1 (MQ=255)
                                                                                 tATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACa                >  1:2740477/1‑101 (MQ=255)
                                                                                 tATCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACa                <  2:2740054/101‑1 (MQ=255)
                                                                                  aTCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAg               <  2:2740167/101‑1 (MQ=255)
                                                                                  aTCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAg               <  2:2739908/101‑1 (MQ=255)
                                                                                   tCCCCAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGa              <  2:2740086/101‑1 (MQ=255)
                                                                                    ccccAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCAt                    <  2:2739798/94‑1 (MQ=255)
                                                                                    ccccAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGaa             >  1:2740481/1‑101 (MQ=255)
                                                                                    ccccAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGaa             <  1:2740035/101‑1 (MQ=255)
                                                                                     cccAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAAt            <  1:2740469/101‑1 (MQ=255)
                                                                                     cccAGCAGGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAAt            >  2:2740482/1‑101 (MQ=255)
                                                                                       cagcagGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGc          <  2:2739850/101‑1 (MQ=255)
                                                                                          cagGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCa       <  1:2740002/101‑1 (MQ=255)
                                                                                           agGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCAt      >  1:2740486/1‑101 (MQ=255)
                                                                                           agGATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCAt      >  2:2740488/1‑101 (MQ=255)
                                                                                             gATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTg    <  2:2740299/101‑1 (MQ=255)
                                                                                             gATAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTg    >  1:2740492/1‑101 (MQ=255)
                                                                                              aTAGCCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGt   >  2:2740493/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CACGAAGCTGCCAAAATTGAAGCCATCGACTTTACCAAAGCCAAACAGCGTGCTGATCCATCCGCCGACTACGGCACCGACTATCCCCAGCAGGATAGTCATAAAGAATCCACCTCCATCTTTACCTGGCATGATCCACTTCGCCAGAATACCGGCAATAAGCCCAAAAATAATCCATGACAGAATGCCCATTGTT  >  NC_000913/1879064‑1879259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑