BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,498	T→C	intergenic (+417/‑110)	rclR → / → ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,498	0	T	C	100.0%	1285.4 / NA	303	intergenic (+417/‑110)	rclR/ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (152/151); total (152/151)

Only 100 of 310 total aligned reads displayed.

ATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAT  >  NC_000913/321400‑321595

 tttCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGggcggt                                                                                                >  1:481578/1‑101 (MQ=255)

   tCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTAt                                                                                              >  2:481580/1‑101 (MQ=255)

    ccccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATg                                                                                             <  2:481325/101‑1 (MQ=255)

     cccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGt                                                                                            <  2:480959/101‑1 (MQ=255)

       cATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTg                                                                                          >  2:481584/1‑101 (MQ=255)

       cATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTg                                                                                          <  2:481110/101‑1 (MQ=255)

        aTAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGc                                                                                         >  1:481585/1‑101 (MQ=255)

        aTAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGc                                                                                         >  2:481588/1‑101 (MQ=255)

          aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTa                                                                                       <  1:480962/101‑1 (MQ=255)

          aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTa                                                                                       >  2:481593/1‑101 (MQ=255)

          aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTa                                                                                       <  2:481045/101‑1 (MQ=255)

           aaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGtt                                                                                           >  1:481594/1‑96 (MQ=255)

            aaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGc                                                                                     >  2:481598/1‑101 (MQ=255)

               gtgAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCaa                                                                                  <  1:481263/101‑1 (MQ=255)

                tgAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAAt                                                                                 >  1:481601/1‑101 (MQ=255)

                tgAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAAt                                                                                 <  1:480955/101‑1 (MQ=255)

                 gAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAAtt                                                                                <  2:480845/101‑1 (MQ=255)

                  aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTa                                                                               >  2:481606/1‑101 (MQ=255)

                  aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTa                                                                               <  1:480880/101‑1 (MQ=255)

                    cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaa                                                                             <  2:481246/101‑1 (MQ=255)

                    cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaa                                                                             >  1:481608/1‑101 (MQ=255)

                      aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaaaa                                                                           <  1:481114/101‑1 (MQ=255)

                      aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaaaa                                                                           <  2:481126/101‑1 (MQ=255)

                      aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaaaa                                                                           <  1:481076/101‑1 (MQ=255)

                      aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaaaa                                                                           <  2:481024/101‑1 (MQ=255)

                       tGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaaaaa                                                                          >  2:481612/1‑101 (MQ=255)

                        gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAg                                                                         <  2:480997/101‑1 (MQ=255)

                        gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAg                                                                         <  1:481106/101‑1 (MQ=255)

                        gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAg                                                                         <  1:481167/101‑1 (MQ=255)

                         ccGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGa                                                                        <  1:481232/101‑1 (MQ=255)

                         ccGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGa                                                                        >  2:481615/1‑101 (MQ=255)

                          cGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGaa                                                                       >  1:481617/1‑101 (MQ=255)

                          cGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGaa                                                                       <  1:480750/101‑1 (MQ=255)

                            aaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTaaaa                                                                            >  2:481630/1‑94 (MQ=255)

                            aaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACg                                                                     >  2:481627/1‑101 (MQ=255)

                            aaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACg                                                                     >  2:481624/1‑101 (MQ=255)

                            aaagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACg                                                                     >  1:481621/1‑101 (MQ=255)

                             aagaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGc                                                                    >  1:481632/1‑101 (MQ=255)

                               gaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCa                                                                  >  2:481635/1‑101 (MQ=255)

                                aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCat                                                                 >  1:481639/1‑101 (MQ=255)

                                aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCat                                                                 <  2:481264/101‑1 (MQ=255)

                                aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCat                                                                 >  2:481641/1‑101 (MQ=255)

                                aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCat                                                                 <  2:481085/101‑1 (MQ=255)

                                 aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                <  2:481409/101‑1 (MQ=255)

                                 aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                <  2:481153/101‑1 (MQ=255)

                                  aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCatat                                                               >  1:481646/1‑101 (MQ=255)

                                    aaaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattt                                                             >  2:481647/1‑101 (MQ=255)

                                     aaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                            >  1:481651/1‑101 (MQ=255)

                                     aaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                            <  1:481129/101‑1 (MQ=255)

                                       aTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatttatt                                                          >  2:481653/1‑101 (MQ=255)

                                         tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtga                                                        <  2:481209/101‑1 (MQ=255)

                                          aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtgat                                                       >  2:481658/1‑101 (MQ=255)

                                           gTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtgatg                                                      >  1:481659/1‑101 (MQ=255)

                                             tATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTgatgat                                                    >  1:481663/1‑101 (MQ=255)

                                                tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattga                                                 <  1:481058/101‑1 (MQ=255)

                                                 gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattgat                                                <  1:481205/101‑1 (MQ=255)

                                                 gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattgat                                                >  2:481667/1‑101 (MQ=255)

                                                 gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattgat                                                <  2:481181/101‑1 (MQ=255)

                                                  cATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATc                                               >  1:481669/1‑101 (MQ=255)

                                                    tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccc                                             >  1:481675/1‑101 (MQ=255)

                                                     ttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATcccc                                            >  2:481676/1‑101 (MQ=255)

                                                      tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccccc                                           <  2:481189/101‑1 (MQ=255)

                                                       gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCg                                          >  1:481680/1‑101 (MQ=255)

                                                          gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGcc                                                                   <  1:481011/73‑1 (MQ=255)

                                                          gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                       >  1:481685/1‑101 (MQ=255)

                                                          gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTg                                       >  2:481684/1‑101 (MQ=255)

                                                           tttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAACCCCCGGTgg                                      <  2:481096/101‑1 (MQ=255)

                                                            ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTggg                                     >  2:481688/1‑101 (MQ=255)

                                                            ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTggg                                     <  1:481401/101‑1 (MQ=255)

                                                                 tGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGa                                >  2:481693/1‑101 (MQ=255)

                                                                 tGAAAGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGa                                >  1:481695/1‑101 (MQ=255)

                                                                    aaGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAc                             >  1:481699/1‑101 (MQ=255)

                                                                    aaGATTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAc                             >  2:481701/1‑101 (MQ=255)

                                                                       aTTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATc                          <  2:481224/101‑1 (MQ=255)

                                                                       aTTATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATc                          >  1:481704/1‑101 (MQ=255)

                                                                         tATCATTTGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCgg                        <  2:481079/101‑1 (MQ=255)

                                                                              tttGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATa                   >  2:481708/1‑101 (MQ=255)

                                                                               ttGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggggg                                  >  1:481711/1‑85 (MQ=255)

                                                                                tGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaaa                 <  1:481030/101‑1 (MQ=255)

                                                                                tGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaaa                 <  1:481185/101‑1 (MQ=255)

                                                                                tGTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaaa                 <  1:481370/101‑1 (MQ=255)

                                                                                 gTCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAt                <  1:481154/101‑1 (MQ=255)

                                                                                  tCATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg               >  2:481713/1‑101 (MQ=255)

                                                                                   cATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg              <  2:481298/101‑1 (MQ=255)

                                                                                   cATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg              <  1:481521/101‑1 (MQ=255)

                                                                                   cATACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg              >  1:481715/1‑101 (MQ=255)

                                                                                     tACAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc            >  2:481719/1‑101 (MQ=255)

                                                                                      aCAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCa           <  2:481235/101‑1 (MQ=255)

                                                                                       cAAATGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCAt          >  2:481722/1‑101 (MQ=255)

                                                                                        aaaTGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg         >  1:481724/1‑101 (MQ=255)

                                                                                        aaaTGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATg         <  1:481278/101‑1 (MQ=255)

                                                                                          aTGAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGaa       <  1:481351/101‑1 (MQ=255)

                                                                                            gAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt     <  1:481098/101‑1 (MQ=255)

                                                                                            gAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt     >  1:481728/1‑101 (MQ=255)

                                                                                            gAGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGt     <  1:481315/101‑1 (MQ=255)

                                                                                             aGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTa    <  2:481217/101‑1 (MQ=255)

                                                                                             aGGGGCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTa    <  1:481340/101‑1 (MQ=255)

                                                                                              ggggCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa   <  1:481063/101‑1 (MQ=255)

                                                                                              ggggCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTaa   <  2:481536/101‑1 (MQ=255)

                                                                                               gggCGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAt  <  2:481168/101‑1 (MQ=255)

ATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAAT  >  NC_000913/321400‑321595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`