BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,431,730	G→A	H405Y (CAC→TAC)	folC ←	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,431,730	0	G	A	100.0%	853.2 / NA	200	H405Y (CAC→TAC)	folC	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (104/96); total (104/96)

Only 100 of 206 total aligned reads displayed.

GCGCCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTGCCGTTACCC  >  NC_000913/2431633‑2431827

gcgcCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGaa                                                                                                >  1:3535229/1‑101 (MQ=255)

gcgcCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGaa                                                                                                >  2:3535230/1‑101 (MQ=255)

 cgcCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGaaa                                                                                               >  1:3535232/1‑101 (MQ=255)

  gcCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAg                                                                                              <  1:3534848/101‑1 (MQ=255)

   ccaccaGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGa                                                                                             <  2:3534858/101‑1 (MQ=255)

    caccaGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTagaaagaa                                                                                            >  1:3535238/1‑101 (MQ=255)

    caccaGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTagaaagaa                                                                                            >  2:3535236/1‑101 (MQ=255)

      ccaGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAAcc                                                                                          >  2:3535239/1‑101 (MQ=255)

        aGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACcac                                                                                        <  2:3534946/101‑1 (MQ=255)

           aCGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACcacaca                                                                                     >  1:3535242/1‑101 (MQ=255)

            cGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCacacac                                                                                    >  1:3535246/1‑101 (MQ=255)

            cGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCacacac                                                                                    >  1:3535244/1‑101 (MQ=255)

              aTCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCa                                                                                  <  2:3534756/101‑1 (MQ=255)

              aTCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCa                                                                                  >  1:3535248/1‑101 (MQ=255)

                cGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGc                                                                                <  1:3534984/101‑1 (MQ=255)

                 gTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCa                                                                               >  2:3535253/1‑101 (MQ=255)

                 gTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCa                                                                               >  1:3535251/1‑101 (MQ=255)

                 gTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCa                                                                               >  1:3535254/1‑101 (MQ=255)

                     ccACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGgt                                                                           <  2:3534923/101‑1 (MQ=255)

                     ccACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGgt                                                                           >  1:3535257/1‑101 (MQ=255)

                      cACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGgtg                                                                          >  1:3535260/1‑101 (MQ=255)

                      cACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGgtg                                                                          >  2:3535259/1‑101 (MQ=255)

                        cTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTc                                                                        <  1:3534953/101‑1 (MQ=255)

                         tAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCt                                                                       <  1:3534932/101‑1 (MQ=255)

                          aaCCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCAAACACCAGCACGGTGTCtt                                                                      >  2:3535263/1‑101 (MQ=255)

                              gATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGc                                                                  >  1:3535265/1‑101 (MQ=255)

                               aTTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCt                                                                 <  1:3535006/101‑1 (MQ=255)

                                 tCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCttt                                                               <  2:3534908/101‑1 (MQ=255)

                                 tCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCttt                                                               >  1:3535267/1‑101 (MQ=255)

                                 tCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCttt                                                               >  2:3535268/1‑101 (MQ=255)

                                   tGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAg                                                             >  1:3535272/1‑101 (MQ=255)

                                    gAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGc                                                            <  1:3534784/101‑1 (MQ=255)

                                    gAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGc                                                            <  2:3535028/101‑1 (MQ=255)

                                    gAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGc                                                            <  1:3535002/101‑1 (MQ=255)

                                       acttacttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTc                                                         >  2:3535273/1‑101 (MQ=255)

                                        cttacttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTcc                                                        >  2:3535275/1‑101 (MQ=255)

                                        cttacttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTcc                                                        <  2:3534921/101‑1 (MQ=255)

                                         ttacttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCg                                                       <  2:3534887/101‑1 (MQ=255)

                                          tacttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGc                                                      <  2:3534988/101‑1 (MQ=255)

                                          tacttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGc                                                      <  1:3534970/101‑1 (MQ=255)

                                            cttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAAc                                                                                           <  2:3535111/62‑1 (MQ=255)

                                            cttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCa                                                    >  1:3535280/1‑101 (MQ=255)

                                            cttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCa                                                    <  2:3534854/101‑1 (MQ=255)

                                             ttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCAt                                                   <  2:3534995/101‑1 (MQ=255)

                                             ttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCAt                                                   >  2:3535282/1‑101 (MQ=255)

                                             ttGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCAt                                                   >  1:3535283/1‑101 (MQ=255)

                                              tGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCAtt                                                  <  1:3534911/101‑1 (MQ=255)

                                               gCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTg                                                 >  2:3535285/1‑101 (MQ=255)

                                               gCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTg                                                 <  2:3534893/101‑1 (MQ=255)

                                               gCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTg                                                 <  2:3534927/101‑1 (MQ=255)

                                                 cACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCg                                               <  1:3534780/101‑1 (MQ=255)

                                                 cACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCg                                               >  1:3535287/1‑101 (MQ=255)

                                                  aCCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCgg                                              >  1:3535289/1‑101 (MQ=255)

                                                   ccGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCg                                                           <  2:3535036/87‑1 (MQ=255)

                                                   ccGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGc                                             >  2:3535291/1‑101 (MQ=255)

                                                    cGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCa                                            <  2:3535059/101‑1 (MQ=255)

                                                     gCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCAt                                           >  2:3535294/1‑101 (MQ=255)

                                                     gCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCAt                                           >  1:3535296/1‑101 (MQ=255)

                                                       ttCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATcc                                         >  1:3535298/1‑101 (MQ=255)

                                                        tctcCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATccc                                        >  2:3535299/1‑101 (MQ=255)

                                                         ctcctcGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCa                                       >  2:3535301/1‑101 (MQ=255)

                                                          tcctcGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGgt                                                                           >  1:3535303/1‑64 (MQ=255)

                                                          tcctcGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCAt                                      <  1:3534935/101‑1 (MQ=255)

                                                          tcctcGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCAt                                      <  1:3535165/101‑1 (MQ=255)

                                                           cctcGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATg                                     <  2:3535001/101‑1 (MQ=255)

                                                            ctcGCGTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGc                                    >  1:3535305/1‑101 (MQ=255)

                                                              cgcgTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCAt                                      <  2:3535011/97‑1 (MQ=255)

                                                               gcgTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTg                                 >  2:3535307/1‑101 (MQ=255)

                                                                cgTCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTgc                                <  2:3534942/101‑1 (MQ=255)

                                                                  tCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTgcgc                              >  1:3535311/1‑101 (MQ=255)

                                                                  tCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTgcgc                              >  2:3535310/1‑101 (MQ=255)

                                                                  tCAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTgcgc                              <  1:3534869/101‑1 (MQ=255)

                                                                   cAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTgcgc                              <  2:3535041/100‑1 (MQ=255)

                                                                   cAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCa                             <  2:3534769/101‑1 (MQ=255)

                                                                   cAATCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCa                             >  1:3535314/1‑101 (MQ=255)

                                                                     aTCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAAc                           <  2:3534816/101‑1 (MQ=255)

                                                                      tCACTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACg                          <  1:3535188/101‑1 (MQ=255)

                                                                       cACTTCGATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGc                         >  1:3535316/1‑101 (MQ=255)

                                                                         cTTCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTa                       >  1:3535318/1‑101 (MQ=255)

                                                                          ttCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCAt                                      <  2:3534826/85‑1 (MQ=255)

                                                                          ttCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTAt                      >  2:3535319/1‑101 (MQ=255)

                                                                           tCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATc                     <  1:3534915/101‑1 (MQ=255)

                                                                           tCCATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATc                     >  1:3535322/1‑101 (MQ=255)

                                                                           tCCATGACATGGGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATc                     >  2:3535323/1‑101 (MQ=255)

                                                                            ccATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCa                    <  2:3534961/101‑1 (MQ=255)

                                                                            ccATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCa                    <  2:3535176/101‑1 (MQ=255)

                                                                             cATGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCaa                   >  2:3535325/1‑101 (MQ=255)

                                                                              aTGACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCaaa                  <  1:3535033/101‑1 (MQ=255)

                                                                                gACATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATg                <  2:3534865/101‑1 (MQ=255)

                                                                                  cATGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGAt              >  1:3535328/1‑101 (MQ=255)

                                                                                   aTGTGCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGAtt             >  2:3535330/1‑101 (MQ=255)

                                                                                    tgtgCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGAttt            >  2:3535332/1‑101 (MQ=255)

                                                                                    tgtgCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGAttt            >  2:3535334/1‑101 (MQ=255)

                                                                                     gtgCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTg           <  1:3534862/101‑1 (MQ=255)

                                                                                      tgCGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTGc          <  1:3535108/101‑1 (MQ=255)

                                                                                        cGACCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTGCCg        >  1:3535335/1‑101 (MQ=255)

                                                                                          aCCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTGCCGtt      <  1:3534809/101‑1 (MQ=255)

                                                                                          aCCGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTGCCGtt      <  2:3534956/101‑1 (MQ=255)

                                                                                            cGTGTAGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCAt                                           <  1:3534884/62‑1 (MQ=255)

                                                                                              tgtaGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTGCCGTTAccc  >  1:3535339/1‑101 (MQ=255)

GCGCCACCAGCACGATCGTGCCCACTAACCGATTCTGAAACTTACTTGCCACCGCTTCTCCTCGCGTCAATCACTTCCATGACATGTGCGACCGTGTGGAAAGAACCACACACCAGCACGGTGTCTTCCGCTTTAGCGTCCGCCATTGCGGCATCCCATGCCTGCGCAACGCTATCAAATGATTTGCCGTTACCC  >  NC_000913/2431633‑2431827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`