Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,438,214 G→A A89T (GCC→ACC)  flk → putative flagella assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,438,2140GA100.0% 1191.8 / NA 287A89T (GCC→ACC) flkputative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (127/160);  total (127/160)

Only 100 of 287 total aligned reads displayed.

GGGCGGGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATTATATCCG  >  NC_000913/2438116‑2438311
                                                                                                  |                                                                                                 
gggCGGGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGAcc                                                                                                 <  1:3544306/101‑1 (MQ=255)
  gCGGGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACcgc                                                                                               <  2:3544313/101‑1 (MQ=255)
   cGGGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACcgcg                                                                                              <  2:3543849/101‑1 (MQ=255)
    gggTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCgcgc                                                                                             >  2:3544643/1‑101 (MQ=255)
     ggTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCa                                                                                            <  1:3544091/101‑1 (MQ=255)
      gTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCaa                                                                                           >  1:3544648/1‑101 (MQ=255)
      gTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCaa                                                                                           >  2:3544650/1‑101 (MQ=255)
        aTCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCaaaa                                                                                         <  1:3544125/101‑1 (MQ=255)
         tCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCaaaaa                                                                                        >  2:3544655/1‑101 (MQ=255)
          cAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCaaaaaa                                                                                       <  2:3543989/101‑1 (MQ=255)
          cAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCaaaaaa                                                                                       >  1:3544657/1‑101 (MQ=255)
            ggCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGc                                                                                     <  2:3544294/101‑1 (MQ=255)
             gCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGcc                                                                                    >  2:3544660/1‑101 (MQ=255)
                cGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCAtt                                                                                 <  1:3544226/101‑1 (MQ=255)
                cGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCAtt                                                                                 >  2:3544664/1‑101 (MQ=255)
                 gATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTc                                                                                <  1:3544010/101‑1 (MQ=255)
                  atatTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCt                                                                               <  2:3544387/101‑1 (MQ=255)
                   tatTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTg                                                                              <  1:3544262/101‑1 (MQ=255)
                   tatTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTg                                                                              >  2:3544669/1‑101 (MQ=255)
                   tatTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTg                                                                              >  1:3544670/1‑101 (MQ=255)
                    atTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGc                                                                             <  2:3544205/101‑1 (MQ=255)
                      tGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGccc                                                                           <  1:3544272/101‑1 (MQ=255)
                      tGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGccc                                                                           <  2:3544330/101‑1 (MQ=255)
                        gTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGc                                                                         <  1:3544373/101‑1 (MQ=255)
                        gTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGc                                                                         <  2:3544075/101‑1 (MQ=255)
                          cggTCGGGAGATTCCCCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAg                                                                                      <  2:3544160/84‑1 (MQ=255)
                          cTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCa                                                                       >  2:3544676/1‑101 (MQ=255)
                          cTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCa                                                                       >  1:3544674/1‑101 (MQ=255)
                           tGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAg                                                                      <  2:3544335/101‑1 (MQ=255)
                           tGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAg                                                                      <  1:3544182/101‑1 (MQ=255)
                              cGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCtt                                                                   <  2:3544302/101‑1 (MQ=255)
                              cGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCtt                                                                   <  1:3543966/101‑1 (MQ=255)
                                 gagaTTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTa                                                                <  2:3544171/101‑1 (MQ=255)
                                 gagaTTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTa                                                                <  2:3544014/101‑1 (MQ=255)
                                   gaTTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAgc                                                              <  1:3544327/101‑1 (MQ=255)
                                   gaTTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAgc                                                              >  1:3544680/1‑101 (MQ=255)
                                   gaTTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAgc                                                              >  2:3544681/1‑101 (MQ=255)
                                      tCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCa                                                           >  1:3544686/1‑101 (MQ=255)
                                      tCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCa                                                           <  2:3544082/101‑1 (MQ=255)
                                      tCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCa                                                           <  2:3544360/101‑1 (MQ=255)
                                       cACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCaa                                                          >  2:3544687/1‑101 (MQ=255)
                                         ccGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAAtt                                                        <  1:3544043/101‑1 (MQ=255)
                                          cGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTa                                                       <  1:3544289/101‑1 (MQ=255)
                                           gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCCGCTTTTAGCGCAATTAg                                                      >  1:3544692/1‑101 (MQ=255)
                                           gctgctTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAg                                                      >  2:3544693/1‑101 (MQ=255)
                                                 ttCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGt                                                <  1:3544348/101‑1 (MQ=255)
                                                  tCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTa                                               <  2:3544619/101‑1 (MQ=255)
                                                    gcgTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTAtt                                             >  1:3544699/1‑101 (MQ=255)
                                                    gcgTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTAtt                                             >  2:3544700/1‑101 (MQ=255)
                                                     cgTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTAttt                                            <  1:3544362/101‑1 (MQ=255)
                                                     cgTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTAttt                                            <  2:3544152/101‑1 (MQ=255)
                                                      gTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTa                                           <  1:3544393/101‑1 (MQ=255)
                                                       tCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTAc                                          <  1:3544238/101‑1 (MQ=255)
                                                        cACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACg                                         >  1:3544703/1‑101 (MQ=255)
                                                          cTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTc                                       <  2:3544259/101‑1 (MQ=255)
                                                           ttCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCt                                      >  1:3544707/1‑101 (MQ=255)
                                                            tCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGAAGTATTTACGTCTg                                     >  2:3544709/1‑101 (MQ=255)
                                                              ccTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTggg                                   >  1:3544712/1‑101 (MQ=255)
                                                               cTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTgggg                                  <  2:3544342/101‑1 (MQ=255)
                                                                  ccGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGaat                               <  1:3544439/101‑1 (MQ=255)
                                                                   cGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTAc                                          <  2:3544216/89‑1 (MQ=255)
                                                                   cGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGt                                        >  2:3544719/1‑91 (MQ=255)
                                                                   cGCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGaata                              >  1:3544717/1‑101 (MQ=255)
                                                                    gCTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGaataa                             <  2:3544197/101‑1 (MQ=255)
                                                                     cTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGaataat                            <  2:3544696/101‑1 (MQ=255)
                                                                     cTGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGaataat                            >  1:3544720/1‑101 (MQ=255)
                                                                      tGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGaataat                            >  2:3544724/1‑100 (MQ=255)
                                                                      tGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATc                           <  2:3544317/101‑1 (MQ=255)
                                                                      tGAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATc                           <  1:3544187/101‑1 (MQ=255)
                                                                       gAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCg                          >  2:3544728/1‑101 (MQ=255)
                                                                       gAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCg                          <  2:3544425/101‑1 (MQ=255)
                                                                       gAGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCg                          <  2:3544135/101‑1 (MQ=255)
                                                                        aGCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGt                         >  1:3544729/1‑101 (MQ=255)
                                                                         gCATAATCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTc                        >  2:3544733/1‑101 (MQ=255)
                                                                             aaTCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGc                    >  2:3544737/1‑101 (MQ=255)
                                                                              aTCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCg                   <  2:3544249/101‑1 (MQ=255)
                                                                              aTCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCg                   >  1:3544738/1‑101 (MQ=255)
                                                                               tCTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCgg                  >  1:3544741/1‑101 (MQ=255)
                                                                                cTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGt                 <  2:3544409/101‑1 (MQ=255)
                                                                                cTGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGt                 >  2:3544743/1‑101 (MQ=255)
                                                                                 tGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTc                        >  2:3544747/1‑93 (MQ=255)
                                                                                 tGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTc                <  1:3544465/101‑1 (MQ=255)
                                                                                 tGGCGCAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTc                <  1:3544168/101‑1 (MQ=255)
                                                                                   gcgcAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTgggg                                  <  1:3544268/81‑1 (MQ=255)
                                                                                   gcgcAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCAc              <  2:3544097/101‑1 (MQ=255)
                                                                                     gcAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGcc                                                                            >  2:3544750/1‑37 (MQ=255)
                                                                                      cAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCtt                                                                   <  2:3544186/45‑1 (MQ=255)
                                                                                      cAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGa           <  2:3544233/101‑1 (MQ=255)
                                                                                      cAACGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGa           <  1:3544554/101‑1 (MQ=255)
                                                                                       aaCGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGAt          <  1:3544666/101‑1 (MQ=255)
                                                                                       aaCGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGAt          >  2:3544753/1‑101 (MQ=255)
                                                                                        aCGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGAtt         <  1:3544415/101‑1 (MQ=255)
                                                                                         cGTCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATta        >  2:3544757/1‑101 (MQ=255)
                                                                                           tCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATtata      <  1:3544162/101‑1 (MQ=255)
                                                                                           tCTGCTGACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATtata      >  2:3544760/1‑101 (MQ=255)
                                                                                            ctgctgACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATTatat     >  1:3544761/1‑101 (MQ=255)
                                                                                             tgctgACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAg                                                      <  1:3544220/51‑1 (MQ=255)
                                                                                             tgctgACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATTATATc    <  2:3544277/101‑1 (MQ=255)
                                                                                             tgctgACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATTATATc    >  2:3544764/1‑101 (MQ=255)
                                                                                               ctgACCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATTATATCCg  >  1:3544768/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GGGCGGGTATCAGGCACGATATTGGTCTGTCGGGAGATTCACCGCTGCTTTCGCGTCACTTCCCTGCCGCTGAGCATAATCTGGCGCAACGTCTGCTGGCCGCGCAAAAAAGCCATTCTGCCCGCCAGCTTTTAGCGCAATTAGGGGAGTATTTACGTCTGGGGAATAATCGTCAGGCGGTCACGGATTATATCCG  >  NC_000913/2438116‑2438311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑