BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,589,492	G→A	W633* (TGG→TAG)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,589,492	0	G	A	100.0%	1099.6 / NA	260	W633* (TGG→TAG)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (146/114); total (146/114)

Only 100 of 263 total aligned reads displayed.

TGAAAAAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGC  >  NC_000913/2589394‑2589589

tGAAAAAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGa                                                                                                 >  2:3768218/1‑101 (MQ=255)

tGAAAAAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGa                                                                                                 >  2:3768220/1‑101 (MQ=255)

 gAAAAAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTagag                                                                                                <  1:3767713/101‑1 (MQ=255)

 gAAAAAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTagag                                                                                                <  1:3767907/101‑1 (MQ=255)

  aaaaaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGc                                                                                               <  2:3767899/101‑1 (MQ=255)

  aaaaaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGc                                                                                               >  2:3768229/1‑101 (MQ=255)

  aaaaaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGc                                                                                               >  1:3768225/1‑101 (MQ=255)

  aaaaaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGc                                                                                               >  1:3768228/1‑101 (MQ=255)

  aaaaaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGc                                                                                               <  1:3767870/101‑1 (MQ=255)

    aaaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGa                                                                                             <  2:3767959/101‑1 (MQ=255)

    aaaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGa                                                                                             >  1:3768232/1‑101 (MQ=255)

     aaGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGaa                                                                                            <  1:3767940/101‑1 (MQ=255)

       gACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAAcg                                                                                          >  2:3768236/1‑101 (MQ=255)

        acaacaTCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAAcgc                                                                                         >  1:3768237/1‑101 (MQ=255)

         caacaTCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAAcgcg                                                                                        >  1:3768240/1‑101 (MQ=255)

           acaTCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGAc                                                                                      >  2:3768245/1‑101 (MQ=255)

           acaTCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGAc                                                                                      >  1:3768243/1‑101 (MQ=255)

             atcatGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGc                                                                                               >  1:3768249/1‑90 (MQ=255)

             atcatGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAg                                                                                    >  1:3768247/1‑101 (MQ=255)

               catGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTa                                                                                  <  1:3767885/101‑1 (MQ=255)

                atGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTaa                                                                                 >  2:3768256/1‑101 (MQ=255)

                atGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTaa                                                                                 >  1:3768255/1‑101 (MQ=255)

                 tGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAg                                                                                <  2:3767750/101‑1 (MQ=255)

                   tCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGAc                                                                              >  1:3768262/1‑101 (MQ=255)

                   tCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGAc                                                                              >  2:3768261/1‑101 (MQ=255)

                     ggTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCg                                                                            >  2:3768265/1‑101 (MQ=255)

                       tgtTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGc                                                                          >  2:3768269/1‑101 (MQ=255)

                        gtTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCa                                                                         <  1:3767788/101‑1 (MQ=255)

                         tttGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCAc                                                                        <  1:3767961/101‑1 (MQ=255)

                          ttGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCAcc                                                                       >  1:3768270/1‑101 (MQ=255)

                            gCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTc                                                                     <  2:3768150/101‑1 (MQ=255)

                               aCCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGtt                                                                  >  2:3768276/1‑101 (MQ=255)

                               aCCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGtt                                                                  >  1:3768275/1‑101 (MQ=255)

                                ccGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTa                                                                                  <  2:3767894/84‑1 (MQ=255)

                                 cGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTg                                                                >  2:3768278/1‑101 (MQ=255)

                                 cGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTg                                                                <  1:3768016/101‑1 (MQ=255)

                                      gTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCAtt                                                           >  1:3768281/1‑101 (MQ=255)

                                        tCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTAt                                                         <  2:3767790/101‑1 (MQ=255)

                                          tGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCg                                                       >  2:3768286/1‑101 (MQ=255)

                                             cccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGc                                                    <  1:3767901/101‑1 (MQ=255)

                                             cccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGc                                                    >  2:3768288/1‑101 (MQ=255)

                                              ccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGc                                                    <  1:3767732/100‑1 (MQ=255)

                                              ccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCg                                                   >  1:3768291/1‑101 (MQ=255)

                                              ccTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCg                                                   >  2:3768292/1‑101 (MQ=255)

                                               cTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGt                                                  >  1:3768297/1‑101 (MQ=255)

                                                tGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTg                                                 >  2:3768299/1‑101 (MQ=255)

                                                 gggggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGc                                                <  1:3767828/101‑1 (MQ=255)

                                                 gggggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGc                                                >  1:3768300/1‑101 (MQ=255)

                                                   gggTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCaa                                              >  2:3768303/1‑101 (MQ=255)

                                                      tAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGa                                           <  2:3767964/101‑1 (MQ=255)

                                                      tAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGa                                           >  1:3768305/1‑101 (MQ=255)

                                                      tAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGa                                           <  2:3767858/101‑1 (MQ=255)

                                                       aaCGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGAc                                                                              >  1:3768309/1‑65 (MQ=255)

                                                         cGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAgg                                        <  2:3767955/101‑1 (MQ=255)

                                                          gggCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCg                                                       >  1:3768313/1‑85 (MQ=255)

                                                          gggCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGc                                       >  2:3768312/1‑101 (MQ=255)

                                                            gCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGt                                     <  1:3767735/101‑1 (MQ=255)

                                                            gCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGt                                     <  1:3767694/101‑1 (MQ=255)

                                                             cAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGtt                                    <  2:3767934/101‑1 (MQ=255)

                                                              aaaaCGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGttt                                   <  1:3767776/101‑1 (MQ=255)

                                                               aaaCGTGGCTCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTa                                  >  1:3768318/1‑101 (MQ=255)

                                                               aaaCGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTa                                  >  2:3768319/1‑101 (MQ=255)

                                                                aaCGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACa                                                                                     >  2:3768322/1‑49 (MQ=255)

                                                                  cGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAgg                                        <  1:3767891/92‑1 (MQ=255)

                                                                  cGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAAcc                               <  2:3767815/101‑1 (MQ=255)

                                                                  cGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAAcc                               >  1:3768325/1‑101 (MQ=255)

                                                                  cGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAAcc                               >  2:3768327/1‑101 (MQ=255)

                                                                  cGTGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAAcc                               <  2:3768084/101‑1 (MQ=255)

                                                                    tGGCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCaa                             >  1:3768329/1‑101 (MQ=255)

                                                                     ggCGCGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCaaa                            <  2:3767835/101‑1 (MQ=255)

                                                                      gcgcGAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAAt                           <  2:3768036/101‑1 (MQ=255)

                                                                       cgcgAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAAtt                          <  1:3767862/101‑1 (MQ=255)

                                                                        gcgAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGa                                                                                       <  2:3767945/39‑1 (MQ=255)

                                                                        gcgAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGt                                                                                   <  2:3767843/43‑1 (MQ=255)

                                                                         cgAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTaa                        >  2:3768333/1‑101 (MQ=255)

                                                                         cgAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTaa                        <  2:3767878/101‑1 (MQ=255)

                                                                         cgAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTaa                        <  1:3768133/101‑1 (MQ=255)

                                                                          gAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCaaa                            <  1:3767839/96‑1 (MQ=255)

                                                                          gAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTaaa                       <  2:3767922/101‑1 (MQ=255)

                                                                          gAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTaaa                       >  2:3768336/1‑101 (MQ=255)

                                                                          gAATGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTaaa                       >  1:3768338/1‑101 (MQ=255)

                                                                           aaTGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAaag                      <  1:3768201/101‑1 (MQ=255)

                                                                            aTGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAaaga                     >  2:3768341/1‑101 (MQ=255)

                                                                            aTGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAaaga                     <  1:3767926/101‑1 (MQ=255)

                                                                             tGTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAaagaa                    <  2:3767996/101‑1 (MQ=255)

                                                                              gTTTATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAaagaag                   <  2:3767909/101‑1 (MQ=255)

                                                                               tttATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGc                  >  1:3768342/1‑101 (MQ=255)

                                                                               tttATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGc                  >  2:3768344/1‑101 (MQ=255)

                                                                                ttATCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCt                 <  2:3767708/101‑1 (MQ=255)

                                                                                  aTCCGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcg               >  2:3768349/1‑101 (MQ=255)

                                                                                    ccGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAg                                         <  2:3767761/73‑1 (MQ=255)

                                                                                    ccGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTcgcg             <  1:3767994/101‑1 (MQ=255)

                                                                                     cGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGt            >  1:3768352/1‑101 (MQ=255)

                                                                                     cGCCTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGt            <  1:3767799/101‑1 (MQ=255)

                                                                                       ccTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAaagaag                   >  2:3768355/1‑92 (MQ=255)

                                                                                       ccTGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTa          >  2:3768353/1‑101 (MQ=255)

                                                                                         tGAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTa          <  1:3768043/99‑1 (MQ=255)

                                                                                          gAAAGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCg       >  2:3768358/1‑101 (MQ=255)

                                                                                             aGACTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCca    >  1:3768363/1‑101 (MQ=255)

                                                                                               aCTAGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCcagc  >  1:3768365/1‑101 (MQ=255)

TGAAAAAGACAACATCATGTCGGTGTTTGCCACCGTTGGTTCTGGCCCTGGGGGTAACGGGCAAAACGTGGCGCGAATGTTTATCCGCCTGAAAGACTGGAGCGAACGCGACAGTAAGACCGGCACCTCGTTTGCCATTATCGAGCGTGCAACGAAGGCGTTTAACCAAATTAAAGAAGCTCGCGTTATCGCCAGC  >  NC_000913/2589394‑2589589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`