BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,280	G→A	G305D (GGC→GAC)	uacT →	urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,280	0	G	A	99.6%	1134.3 / NA	273	G305D (GGC→GAC)	uacT	urate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (134/138); minor base C (1/0); total (135/138)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

GGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCG  >  NC_000913/3032182‑3032377

ggggATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAgacg                                                                                                 <  2:4425398/101‑1 (MQ=255)

ggggATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAgacg                                                                                                 <  2:4425419/101‑1 (MQ=255)

  ggATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAgacggc                                                                                               >  2:4425763/1‑101 (MQ=255)

   gATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCa                                                                                              >  1:4425764/1‑101 (MQ=255)

       ttCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGtt                                                                                          >  2:4425768/1‑101 (MQ=255)

         ccTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTa                                                                                        <  2:4425303/101‑1 (MQ=255)

         ccTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTa                                                                                        >  1:4425770/1‑101 (MQ=255)

          cTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTaa                                                                                       <  1:4425424/101‑1 (MQ=255)

            ggCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACa                                                                                     <  2:4425180/101‑1 (MQ=255)

            ggCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACa                                                                                     <  1:4425327/101‑1 (MQ=255)

            ggCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACa                                                                                     <  1:4425613/101‑1 (MQ=255)

             gCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTa                                                                                        >  1:4425774/1‑97 (MQ=255)

               aCTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCt                                                                                  >  2:4425775/1‑101 (MQ=255)

                 tGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTc                                                                                >  1:4425778/1‑101 (MQ=255)

                 tGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTc                                                                                >  1:4425781/1‑101 (MQ=255)

                 tGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTc                                                                                >  2:4425782/1‑101 (MQ=255)

                    gTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTcccc                                                                             >  1:4425786/1‑101 (MQ=255)

                     tGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTccccc                                                                            <  1:4425274/101‑1 (MQ=255)

                      gAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCca                                                                           >  1:4425790/1‑101 (MQ=255)

                      gAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCca                                                                           >  2:4425793/1‑101 (MQ=255)

                       aaaTAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCcac                                                                          <  1:4425140/101‑1 (MQ=255)

                       aaaTAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCcac                                                                          >  1:4425796/1‑101 (MQ=255)

                        aaTAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCcaca                                                                         <  2:4425249/101‑1 (MQ=255)

                        aaTAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCcaca                                                                         >  2:4425797/1‑101 (MQ=255)

                         aTAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCacac                                                                        <  1:4425364/101‑1 (MQ=255)

                          tAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACg                                                                       >  1:4425800/1‑101 (MQ=255)

                          tAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACg                                                                       <  2:4425430/101‑1 (MQ=255)

                          tAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACg                                                                       <  1:4425351/101‑1 (MQ=255)

                           aGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGt                                                                      >  1:4425805/1‑101 (MQ=255)

                           aGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGt                                                                      >  2:4425803/1‑101 (MQ=255)

                            gtcggtcgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTc                                                                     <  2:4425380/101‑1 (MQ=255)

                             tcggtcgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCa                                                                    >  2:4425808/1‑101 (MQ=255)

                                gtcgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCAttt                                                                 >  1:4425811/1‑101 (MQ=255)

                                  cgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTtc                                                               <  2:4425324/101‑1 (MQ=255)

                                   gTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCAt                                                                   <  2:4425362/96‑1 (MQ=255)

                                   gTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTtct                                                              >  1:4425814/1‑101 (MQ=255)

                                    tAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTtctc                                                             >  2:4425817/1‑101 (MQ=255)

                                    tAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTtctc                                                             <  2:4425393/101‑1 (MQ=255)

                                       aCTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCaaa                                                          >  1:4425821/1‑101 (MQ=255)

                                         tctctTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAAc                                                        >  1:4425825/1‑101 (MQ=255)

                                         tctctTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAAc                                                        <  1:4425576/101‑1 (MQ=255)

                                         tctctTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCACCCACACGTCATTTTCTCAAAAc                                                        >  2:4425824/1‑101 (MQ=255)

                                           tctTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGt                                                      <  2:4425280/101‑1 (MQ=255)

                                            ctTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGtt                                                     >  1:4425829/1‑101 (MQ=255)

                                            ctTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGtt                                                     >  2:4425830/1‑101 (MQ=255)

                                                 cACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCt                                                <  1:4425599/101‑1 (MQ=255)

                                                  aCGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTg                                               <  1:4425532/101‑1 (MQ=255)

                                                    gATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGt                                             >  1:4425836/1‑101 (MQ=255)

                                                     atatTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGtt                                            >  1:4425839/1‑101 (MQ=255)

                                                     atatTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGtt                                            >  2:4425840/1‑101 (MQ=255)

                                                      tattatTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCt                                                <  1:4425546/96‑1 (MQ=255)

                                                      tattatTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTa                                           <  1:4425186/101‑1 (MQ=255)

                                                      tattatTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTa                                           <  1:4425232/101‑1 (MQ=255)

                                                       attattCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAg                                          >  1:4425844/1‑101 (MQ=255)

                                                        ttattCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGc                                         >  1:4425846/1‑101 (MQ=255)

                                                         tattCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCg                                        >  1:4425850/1‑101 (MQ=255)

                                                             cgcgGGCGGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGAc                                    >  1:4425853/1‑101 (MQ=255)

                                                               cgGGCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTa                                                                                        <  2:4425066/47‑1 (MQ=255)

                                                                 ggCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGAcgcgc                                <  2:4425517/101‑1 (MQ=255)

                                                                 ggCTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGAcgcgc                                >  1:4425856/1‑101 (MQ=255)

                                                                   cTGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGt                              >  2:4425858/1‑101 (MQ=255)

                                                                    tGCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGtt                             >  1:4425862/1‑101 (MQ=255)

                                                                    tGCGTGTCCATGGCGTAGGGACAATGATAGACGGCACGTTTaa                                                                                       >  2:4425864/1‑43 (MQ=255)

                                                                     gCGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTc                            >  1:4425865/1‑101 (MQ=255)

                                                                      cGTGTCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCa                           >  2:4425869/1‑101 (MQ=255)

                                                                       gtgtCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCAt                          <  1:4425519/101‑1 (MQ=255)

                                                                         gtCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAg                        <  1:4425387/101‑1 (MQ=255)

                                                                         gtCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAg                        <  1:4425427/101‑1 (MQ=255)

                                                                         gtCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAg                        <  2:4425676/101‑1 (MQ=255)

                                                                         gtCGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAg                        <  2:4425616/101‑1 (MQ=255)

                                                                           cGATGGCGTAGGGCCAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGcc                      >  1:4425872/1‑101 (MQ=255)

                                                                           cGATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGcc                      <  1:4425321/101‑1 (MQ=255)

                                                                            gATGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCg                     >  2:4425874/1‑101 (MQ=255)

                                                                             aTGGCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGc                    <  2:4425478/101‑1 (MQ=255)

                                                                               ggCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCt                   <  2:4425339/100‑1 (MQ=255)

                                                                               ggCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTg                  <  2:4425441/101‑1 (MQ=255)

                                                                               ggCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTg                  >  1:4425878/1‑101 (MQ=255)

                                                                               ggCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTg                  <  1:4425448/101‑1 (MQ=255)

                                                                               ggCGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTg                  <  1:4425399/101‑1 (MQ=255)

                                                                                gCGTAGGGACAATGATAGACGACACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTgg                 >  2:4425879/1‑101 (MQ=255)

                                                                                 cGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTggg                <  2:4425604/101‑1 (MQ=255)

                                                                                 cGTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTggg                <  1:4425504/101‑1 (MQ=255)

                                                                                  gTAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgt               <  2:4425295/101‑1 (MQ=255)

                                                                                   tAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgtg              <  2:4425239/101‑1 (MQ=255)

                                                                                   tAGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgtg              >  1:4425883/1‑101 (MQ=255)

                                                                                    aGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgtgt             >  2:4425886/1‑101 (MQ=255)

                                                                                    aGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgtgt             <  1:4425537/101‑1 (MQ=255)

                                                                                    aGGGACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGgtgt             <  2:4425534/101‑1 (MQ=255)

                                                                                       gACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTa          >  1:4425888/1‑101 (MQ=255)

                                                                                       gACAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTa          <  1:4425567/101‑1 (MQ=255)

                                                                                        aCAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGc                                                  <  2:4425406/60‑1 (MQ=255)

                                                                                        aCAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCt                   <  1:4425643/91‑1 (MQ=255)

                                                                                        aCAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTAt         <  2:4425560/101‑1 (MQ=255)

                                                                                         cAATGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTAtt        >  1:4425892/1‑101 (MQ=255)

                                                                                          aaTGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTAttt       <  2:4425562/101‑1 (MQ=255)

                                                                                          aaTGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTAttt       >  2:4425894/1‑101 (MQ=255)

                                                                                           aTGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATttc      <  2:4425756/101‑1 (MQ=255)

                                                                                            tGATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATttct     <  2:4425464/101‑1 (MQ=255)

                                                                                             gATAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATttctt    >  1:4425898/1‑101 (MQ=255)

                                                                                               tAGACGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCg  <  2:4425501/101‑1 (MQ=255)

GGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCG  >  NC_000913/3032182‑3032377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`