BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,593,495	G→A	P238S (CCG→TCG)	livG ←	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,593,495	0	G	A	100.0%	1171.9 / NA	280	P238S (CCG→TCG)	livG	branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivG
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (142/138); total (142/138)

Only 100 of 280 total aligned reads displayed.

TGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGATAGTG  >  NC_000913/3593397‑3593592

 gggCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGt                                                                                                >  2:5275932/1‑101 (MQ=255)

 gggCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGt                                                                                                <  1:5275452/101‑1 (MQ=255)

 gggCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGt                                                                                                <  2:5275486/101‑1 (MQ=255)

  ggCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTa                                                                                               >  1:5275935/1‑101 (MQ=255)

  ggCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTa                                                                                               <  1:5275734/101‑1 (MQ=255)

     gcTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCg                                                                                            >  2:5275937/1‑101 (MQ=255)

       tGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGtt                                                                                          <  1:5275243/101‑1 (MQ=255)

        gACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGttt                                                                                         >  1:5275943/1‑101 (MQ=255)

        gACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGttt                                                                                         >  2:5275944/1‑101 (MQ=255)

        gACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGttt                                                                                         <  1:5275652/101‑1 (MQ=255)

            ttGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCa                                                                                     <  1:5275534/101‑1 (MQ=255)

             tGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAg                                                                                    >  1:5275948/1‑101 (MQ=255)

             tGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAg                                                                                    <  2:5275454/101‑1 (MQ=255)

              gTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgc                                                                                   <  1:5275506/101‑1 (MQ=255)

               tCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgcg                                                                                  <  1:5275489/101‑1 (MQ=255)

                cAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgcgg                                                                                 >  2:5275950/1‑101 (MQ=255)

                cAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgcgg                                                                                 <  1:5275602/101‑1 (MQ=255)

                 aaaGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgcggc                                                                                <  1:5275440/101‑1 (MQ=255)

                  aaGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgcggcg                                                                               >  2:5275954/1‑101 (MQ=255)

                  aaGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgcggcg                                                                               >  1:5275955/1‑101 (MQ=255)

                      acaacaTGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGt                                                                                           >  2:5275961/1‑85 (MQ=255)

                       caacaTGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTcccc                                                                          >  1:5275962/1‑101 (MQ=255)

                            tGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGAtt                                                                     >  1:5275967/1‑101 (MQ=255)

                                tttttCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGAcc                                                                 >  1:5275969/1‑101 (MQ=255)

                                tttttCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGAcc                                                                 >  2:5275970/1‑101 (MQ=255)

                                tttttCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGAcc                                                                 <  1:5275573/101‑1 (MQ=255)

                                  tttCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCAc                                                               <  2:5275344/101‑1 (MQ=255)

                                    tCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGt                                                             >  2:5275975/1‑101 (MQ=255)

                                    tCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGt                                                             <  2:5275557/101‑1 (MQ=255)

                                    tCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGt                                                             >  1:5275976/1‑101 (MQ=255)

                                     ccATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTa                                                            >  1:5275979/1‑101 (MQ=255)

                                       aTCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAgcg                                                                                  >  2:5275986/1‑77 (MQ=255)

                                       aTCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTaaa                                                          >  2:5275984/1‑101 (MQ=255)

                                        tCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAAt                                                         >  1:5275989/1‑101 (MQ=255)

                                           tATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCg                                                      >  1:5275992/1‑101 (MQ=255)

                                            aTGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCgg                                                     >  2:5275993/1‑101 (MQ=255)

                                              gCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTc                                                   >  1:5275996/1‑101 (MQ=255)

                                              gCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTc                                                   <  1:5275746/101‑1 (MQ=255)

                                               ccTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTcc                                                  <  2:5275569/101‑1 (MQ=255)

                                               ccTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTcc                                                  >  2:5276000/1‑101 (MQ=255)

                                                 tCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGa                                                <  1:5275720/101‑1 (MQ=255)

                                                  cACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAg                                                                                    <  1:5275671/64‑1 (MQ=255)

                                                   aCCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGaaa                                              <  2:5275833/101‑1 (MQ=255)

                                                   aCCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGaaa                                              >  1:5276004/1‑101 (MQ=255)

                                                   aCCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGaaa                                              <  1:5275940/101‑1 (MQ=255)

                                                    ccTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAAt                                             <  1:5275645/101‑1 (MQ=255)

                                                    ccTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAAt                                             <  1:5275199/101‑1 (MQ=255)

                                                     cTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAAtt                                            >  1:5276008/1‑101 (MQ=255)

                                                     cTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAAtt                                            >  2:5276007/1‑101 (MQ=255)

                                                      tAAATAGGCGCGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGaa                                               <  1:5275626/97‑1 (MQ=255)

                                                      tAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTc                                           >  1:5276013/1‑101 (MQ=255)

                                                       aaaTAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTcc                                          >  1:5276017/1‑101 (MQ=255)

                                                       aaaTAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTcc                                          >  2:5276015/1‑101 (MQ=255)

                                                          tAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCAt                                       <  1:5275593/101‑1 (MQ=255)

                                                           aGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATc                                      >  2:5276020/1‑101 (MQ=255)

                                                           aGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATc                                      >  1:5276021/1‑101 (MQ=255)

                                                            ggCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCa                                     <  2:5275423/101‑1 (MQ=255)

                                                             gCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCAc                                    <  1:5275410/101‑1 (MQ=255)

                                                              cACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCAcc                                   >  1:5276025/1‑101 (MQ=255)

                                                              cACGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCAcc                                   <  2:5275756/101‑1 (MQ=255)

                                                                cGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCa                                  <  2:5275535/100‑1 (MQ=255)

                                                                cGGATCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAg                                 >  2:5276027/1‑101 (MQ=255)

                                                                   aTCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCtt                              >  1:5276032/1‑101 (MQ=255)

                                                                   aTCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCtt                              <  2:5275582/101‑1 (MQ=255)

                                                                   aTCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCtt                              <  2:5275656/101‑1 (MQ=255)

                                                                    tCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAg                                 <  1:5275801/97‑1 (MQ=255)

                                                                    tCACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTc                             >  2:5276034/1‑101 (MQ=255)

                                                                     cACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCa                            >  2:5276038/1‑101 (MQ=255)

                                                                     cACGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCa                            >  1:5276039/1‑101 (MQ=255)

                                                                      aCGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCat                           >  2:5276042/1‑101 (MQ=255)

                                                                      aCGTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCat                           <  2:5275589/101‑1 (MQ=255)

                                                                        gTCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTcc                                          >  1:5276046/1‑84 (MQ=255)

                                                                         tCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCatat                         >  1:5276048/1‑100 (MQ=255)

                                                                         tCCGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATc                        <  1:5275657/101‑1 (MQ=255)

                                                                          ccGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCg                       <  2:5275743/101‑1 (MQ=255)

                                                                          ccGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCg                       <  2:5275493/101‑1 (MQ=255)

                                                                           cGGGTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCgt                      >  2:5276051/1‑101 (MQ=255)

                                                                            gggTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCgtg                     >  1:5276053/1‑101 (MQ=255)

                                                                            gggTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCgtg                     >  2:5276055/1‑101 (MQ=255)

                                                                             ggTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCgtgt                    <  2:5275633/101‑1 (MQ=255)

                                                                             ggTTATTACGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCgtgt                    <  2:5275786/101‑1 (MQ=255)

                                                                               ttattaCGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTtc                  <  2:5275856/101‑1 (MQ=255)

                                                                               ttattaCGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTtc                  <  2:5275546/101‑1 (MQ=255)

                                                                                tattaCGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTtca                 <  1:5275545/101‑1 (MQ=255)

                                                                                tattaCGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTtca                 <  2:5275517/101‑1 (MQ=255)

                                                                                  ttaCGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTtcaat               <  1:5275585/101‑1 (MQ=255)

                                                                                   taCGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTtcaatc              >  1:5276060/1‑101 (MQ=255)

                                                                                    aCGGATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTtcaatca             >  2:5276061/1‑101 (MQ=255)

                                                                                      ggATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATcaac           <  2:5275699/101‑1 (MQ=255)

                                                                                       gATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATcaaca          <  2:5275607/101‑1 (MQ=255)

                                                                                       gATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATcaaca          <  2:5275772/101‑1 (MQ=255)

                                                                                       gATCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATcaaca          <  2:5275724/101‑1 (MQ=255)

                                                                                        aTCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATcaacaa         >  1:5276065/1‑101 (MQ=255)

                                                                                         tCTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAg        <  1:5275690/101‑1 (MQ=255)

                                                                                          cTGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGa       <  2:5275636/101‑1 (MQ=255)

                                                                                           tGCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGAt      >  1:5276069/1‑101 (MQ=255)

                                                                                            gCTCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGATa     >  2:5276070/1‑101 (MQ=255)

                                                                                             cTCCGACGTACCGTCTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGATAg    <  1:5275779/101‑1 (MQ=255)

                                                                                              tCCGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGATAgt   >  1:5276073/1‑101 (MQ=255)

                                                                                               ccGACGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGATAgtg  <  2:5275676/101‑1 (MQ=255)

TGGGCGCTGACTTTGTCAAAGGACAACATGACTTTTTCCATCTTATGCCTCACCTAAATAGGCACGGATCACGTCCGGGTTATTACGGATCTGCTCCGGCGTACCGTTTGCCAGCGGCGTCCCCTGATTGACCACGTAAATTCGGTCCGAAATTCCCATCACCAGCTTCATATCGTGTTCAATCAACAAGATAGTG  >  NC_000913/3593397‑3593592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`