BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,810,786	G→A	H123Y (CAT→TAT)	mutM ←	DNA‑formamidopyrimidine glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,810,786	0	G	A	99.3%	1123.5 / NA	289	H123Y (CAT→TAT)	mutM	DNA‑formamidopyrimidine glycosylase
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (136/149); minor base C (0/2); total (136/153)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

TTTAATCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATGCCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCA  >  NC_000913/3810688‑3810883

tttAATCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATAcc                                                                                                 >  2:5624576/1‑101 (MQ=255)

 ttAATCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATAccc                                                                                                >  1:5624578/1‑101 (MQ=255)

  tAATCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCt                                                                                               >  1:5624582/1‑101 (MQ=255)

  tAATCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCt                                                                                               <  2:5624353/101‑1 (MQ=255)

  tAATCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCt                                                                                               <  1:5624206/101‑1 (MQ=255)

   aaTCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCtt                                                                                              <  1:5624415/101‑1 (MQ=255)

   aaTCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCtt                                                                                              >  2:5624584/1‑101 (MQ=255)

     tCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTcc                                                                                            <  2:5624140/101‑1 (MQ=255)

     tCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTcc                                                                                            <  2:5624119/101‑1 (MQ=255)

      cGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCa                                                                                           >  2:5624589/1‑101 (MQ=255)

      cGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCa                                                                                           <  2:5624213/101‑1 (MQ=255)

      cGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCa                                                                                           <  2:5624312/101‑1 (MQ=255)

       gCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAg                                                                                          >  1:5624592/1‑101 (MQ=255)

       gCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAg                                                                                          <  2:5623972/101‑1 (MQ=255)

         cGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGct                                                                                        <  1:5624184/101‑1 (MQ=255)

         cGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGct                                                                                        <  2:5624243/101‑1 (MQ=255)

          gTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGctc                                                                                       >  2:5624594/1‑101 (MQ=255)

            ttttttCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCtt                                                                                     >  2:5624597/1‑101 (MQ=255)

             tttttCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCttt                                                                                    <  2:5624316/101‑1 (MQ=255)

             tttttCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCttt                                                                                    >  1:5624601/1‑101 (MQ=255)

              ttttCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTg                                                                                   >  2:5624604/1‑101 (MQ=255)

               tttCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTgg                                                                                  >  1:5624606/1‑101 (MQ=255)

               tttCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTgg                                                                                  <  1:5624226/101‑1 (MQ=255)

                ttcttcGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGt                                                                                 <  1:5624332/101‑1 (MQ=255)

                    tcGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGt                                                                                 >  2:5624610/1‑97 (MQ=255)

                     cgcgCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCcaca                                                                            <  1:5624382/101‑1 (MQ=255)

                      gcgcACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAg                                                                           <  1:5624325/101‑1 (MQ=255)

                       cgcACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGc                                                                          >  1:5624614/1‑101 (MQ=255)

                        gcACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGcc                                                                         <  1:5624114/101‑1 (MQ=255)

                         cACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCa                                                                        >  1:5624616/1‑101 (MQ=255)

                         cACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCa                                                                        >  2:5624617/1‑101 (MQ=255)

                           cTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAgg                                                                      <  2:5624255/101‑1 (MQ=255)

                           cTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAgg                                                                      <  2:5623913/101‑1 (MQ=255)

                             tCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCa                                                                    <  2:5624233/101‑1 (MQ=255)

                              cTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCAc                                                                   >  1:5624620/1‑101 (MQ=255)

                                gATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCa                                                                 >  1:5624624/1‑101 (MQ=255)

                                  tGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCaaa                                                               <  2:5624266/101‑1 (MQ=255)

                                   gCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAg                                                              <  2:5624192/101‑1 (MQ=255)

                                   gCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAg                                                              <  2:5624373/101‑1 (MQ=255)

                                   gCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAg                                                              >  1:5624628/1‑101 (MQ=255)

                                    cAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgc                                                             <  2:5624097/101‑1 (MQ=255)

                                    cAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgc                                                             <  1:5623873/101‑1 (MQ=255)

                                    cAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgc                                                             >  2:5624631/1‑101 (MQ=255)

                                     aGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgcg                                                            <  2:5624307/101‑1 (MQ=255)

                                     aGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgcg                                                            >  1:5624633/1‑101 (MQ=255)

                                        tACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgcggcg                                                         >  2:5624637/1‑101 (MQ=255)

                                        tACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgcggcg                                                         <  2:5624298/101‑1 (MQ=255)

                                         aCTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGgcgc                                                        >  1:5624638/1‑101 (MQ=255)

                                           tCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCgg                                                      >  2:5624640/1‑101 (MQ=255)

                                           tCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCgg                                                      <  2:5624176/101‑1 (MQ=255)

                                            cACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGa                                                     <  2:5624168/101‑1 (MQ=255)

                                             aCCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGAt                                                    <  1:5624067/101‑1 (MQ=255)

                                             aCCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGAt                                                    <  1:5624299/101‑1 (MQ=255)

                                              ccATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATc                                                   <  2:5624143/101‑1 (MQ=255)

                                              ccATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATc                                                   <  2:5624070/101‑1 (MQ=255)

                                              ccATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATc                                                   <  2:5624022/101‑1 (MQ=255)

                                                aTTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCgg                                                 >  2:5624644/1‑101 (MQ=255)

                                                 ttGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGgt                                                >  1:5624647/1‑101 (MQ=255)

                                                   gAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGgtgt                                              <  2:5624159/101‑1 (MQ=255)

                                                    aaaTCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTa                                             <  2:5624172/101‑1 (MQ=255)

                                                    aaaTCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTa                                             >  1:5624650/1‑101 (MQ=255)

                                                      aTCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAgc                                           >  1:5624653/1‑101 (MQ=255)

                                                       tcgtcgCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAgcg                                          <  2:5624487/101‑1 (MQ=255)

                                                       tcgtcgCTAAGCGGCTCAGGTCCAAGATGGGTCAGCACATTATGCCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAgc                                                             <  2:5624088/82‑1 (MQ=255)

                                                           cgCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCgcagc                                      >  1:5624657/1‑101 (MQ=255)

                                                            gCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCgcagca                                     >  2:5624661/1‑101 (MQ=255)

                                                            gCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCgcagca                                     <  2:5624271/101‑1 (MQ=255)

                                                            gCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCgcagca                                     >  1:5624660/1‑101 (MQ=255)

                                                             cTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCAc                                    >  1:5624664/1‑101 (MQ=255)

                                                              tAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACt                                   >  1:5624667/1‑101 (MQ=255)

                                                                aGCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACttt                                 <  2:5624434/101‑1 (MQ=255)

                                                                 gCGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTg                                <  2:5624389/101‑1 (MQ=255)

                                                                  cGGCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGc                               <  1:5624368/101‑1 (MQ=255)

                                                                   ggCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGcc                              >  2:5624671/1‑101 (MQ=255)

                                                                    gCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCg                             <  2:5624286/101‑1 (MQ=255)

                                                                    gCTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCg                             <  1:5624242/101‑1 (MQ=255)

                                                                     cTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGt                            >  2:5624674/1‑101 (MQ=255)

                                                                     cTCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGt                            >  1:5624675/1‑101 (MQ=255)

                                                                      tCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGtt                           <  2:5624402/101‑1 (MQ=255)

                                                                      tCCGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGtt                           <  1:5624093/101‑1 (MQ=255)

                                                                        cGGTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGc                         >  1:5624680/1‑101 (MQ=255)

                                                                         ggTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCt                        <  2:5624336/101‑1 (MQ=255)

                                                                         ggTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCt                        >  2:5624681/1‑101 (MQ=255)

                                                                          gTCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCtc                       <  1:5624013/101‑1 (MQ=255)

                                                                           tCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCtca                      >  1:5624686/1‑101 (MQ=255)

                                                                           tCCAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCtca                      >  2:5624688/1‑101 (MQ=255)

                                                                            ccAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCtcat                     >  1:5624690/1‑101 (MQ=255)

                                                                            ccAAGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCtcat                     <  1:5624219/101‑1 (MQ=255)

                                                                              aaGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCtcatca                   >  2:5624693/1‑101 (MQ=255)

                                                                               aGATGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCAc                  <  1:5624163/101‑1 (MQ=255)

                                                                                 aTGGGTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCa                >  2:5624697/1‑101 (MQ=255)

                                                                                   gggTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCaaa              <  1:5624151/101‑1 (MQ=255)

                                                                                    ggTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAAt             >  1:5624700/1‑101 (MQ=255)

                                                                                    ggTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAAt             >  2:5624702/1‑101 (MQ=255)

                                                                                     gTCAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATc            <  1:5624263/101‑1 (MQ=255)

                                                                                       cAGCACATTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCca          >  2:5624705/1‑101 (MQ=255)

                                                                                          cacaTTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACAt       >  1:5624708/1‑101 (MQ=255)

                                                                                          cacaTTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACAt       <  1:5624282/101‑1 (MQ=255)

                                                                                             aTTATACCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGt    >  2:5624712/1‑101 (MQ=255)

                                                                                               tataCCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCa  >  1:5624714/1‑101 (MQ=255)

TTTAATCGCCGTTTTTTTCTTCGCGCACTTCTGATGCAGATACTCACCATTGAAATCGTCGCTAAGCGGCTCCGGTCCAAGATGGGTCAGCACATTATGCCCTTCCAGCTCTTTGGTCCACAGCCAGGCACCAAAGCGGCGCGGATCGGTGTAGCGCAGCACTTTGCCGTTGCTCATCACCAAATCCACATGGTCA  >  NC_000913/3810688‑3810883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`