Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 158,294 G→A T278I (ACC→ATC)  pcnB ← poly(A) polymerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913158,2940GA99.6% 1150.9 / ‑3.9 272T278I (ACC→ATC) pcnBpoly(A) polymerase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (120/151);  minor base T (0/1);  total (120/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 272 total aligned reads displayed.

GCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGTCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAG  >  NC_000913/158196‑158391
                                                                                                  |                                                                                                 
  atatCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCgg                                                                                               <  2:234186/101‑1 (MQ=255)
  atatCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCgg                                                                                               >  1:234675/1‑101 (MQ=255)
    atCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGa                                                                                             >  1:234680/1‑101 (MQ=255)
    atCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGa                                                                                             <  2:234353/101‑1 (MQ=255)
    atCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGa                                                                                             >  2:234679/1‑101 (MQ=255)
      cGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAAc                                                                                           <  1:234280/101‑1 (MQ=255)
      cGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAAc                                                                                           <  2:234423/101‑1 (MQ=255)
       gTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACa                                                                                          <  1:234356/101‑1 (MQ=255)
         tATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGc                                                                                        >  1:234689/1‑101 (MQ=255)
         tATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGc                                                                                        <  2:234371/101‑1 (MQ=255)
         tATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGc                                                                                        >  2:234688/1‑101 (MQ=255)
         tATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGc                                                                                        >  2:234686/1‑101 (MQ=255)
           tGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCgg                                                                                      <  2:234299/101‑1 (MQ=255)
           tGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCgg                                                                                      >  2:234693/1‑101 (MQ=255)
            ggATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCgg                                                                                      <  2:234397/100‑1 (MQ=255)
             gATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCt                                                                                    <  2:234226/101‑1 (MQ=255)
              aTACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTg                                                                                   <  2:234470/101‑1 (MQ=255)
               tACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTgg                                                                                  <  2:234275/101‑1 (MQ=255)
                aCGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGa                                                                                 >  1:234697/1‑101 (MQ=255)
                aCGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGa                                                                                 >  2:234698/1‑101 (MQ=255)
                 cgcgTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGaa                                                                                <  1:234120/101‑1 (MQ=255)
                  gcgTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACa                                                                                          >  1:234704/1‑90 (MQ=255)
                  gcgTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAAc                                                                               >  1:234701/1‑101 (MQ=255)
                   cgTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACa                                                                              <  2:234359/101‑1 (MQ=255)
                    gTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAg                                                                             >  1:234707/1‑101 (MQ=255)
                     tATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAga                                                                            >  2:234709/1‑101 (MQ=255)
                     tATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAga                                                                            >  1:234711/1‑101 (MQ=255)
                     tATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAga                                                                            <  2:234415/101‑1 (MQ=255)
                      aTCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAgat                                                                           <  1:234216/101‑1 (MQ=255)
                      aTCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAgat                                                                           <  2:234490/101‑1 (MQ=255)
                        cGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAgatga                                                                         >  1:234715/1‑101 (MQ=255)
                         ggTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAgatgat                                                                        <  1:234372/101‑1 (MQ=255)
                          gTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGata                                                                       <  1:234322/101‑1 (MQ=255)
                          gTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGata                                                                       <  1:234152/101‑1 (MQ=255)
                           tATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGatat                                                                      >  2:234719/1‑101 (MQ=255)
                           tATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGatat                                                                      >  1:234718/1‑101 (MQ=255)
                            aTTCTTCAGCACCTGGTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATAtt                                                                     <  1:234511/101‑1 (MQ=255)
                              tcttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTca                                                                   <  2:234201/101‑1 (MQ=255)
                              tcttcAGCACCTGTTCAATGAGCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTca                                                                   >  1:234725/1‑101 (MQ=255)
                               cttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTcac                                                                  <  2:234286/101‑1 (MQ=255)
                                ttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATAtt                                                                     >  2:234731/1‑97 (MQ=255)
                                ttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTcaca                                                                 >  1:234728/1‑101 (MQ=255)
                                ttcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTcaca                                                                 >  1:234730/1‑101 (MQ=255)
                                 tcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAga                                                                            >  2:234734/1‑89 (MQ=255)
                                 tcAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTcacac                                                                <  2:234331/101‑1 (MQ=255)
                                  cAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTcacaca                                                               >  2:234736/1‑101 (MQ=255)
                                  cAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTcacaca                                                               <  2:234406/101‑1 (MQ=255)
                                    gCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACacaac                                                             >  1:234741/1‑101 (MQ=255)
                                     cACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACacaaca                                                            <  1:234332/101‑1 (MQ=255)
                                      aCCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAg                                                           <  1:234443/101‑1 (MQ=255)
                                      aCCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAg                                                           >  1:234745/1‑101 (MQ=255)
                                        cTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCt                                                         <  1:234426/101‑1 (MQ=255)
                                        cTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCt                                                         <  2:234477/101‑1 (MQ=255)
                                        cTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCt                                                         <  2:234308/101‑1 (MQ=255)
                                         tGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAgatg                                                                          >  2:234749/1‑83 (MQ=255)
                                         tGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCtt                                                        <  1:234079/101‑1 (MQ=255)
                                         tGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCtt                                                        <  2:234654/101‑1 (MQ=255)
                                         tGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCtt                                                        <  1:234479/101‑1 (MQ=255)
                                          gTTCAATGAGCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTta                                                       >  1:234750/1‑101 (MQ=255)
                                            tCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTtata                                                     >  1:234752/1‑101 (MQ=255)
                                             cAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAg                                                    <  2:234411/101‑1 (MQ=255)
                                                tGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGtt                                                 >  2:234757/1‑101 (MQ=255)
                                                   tCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAgat                                                                           <  2:234327/72‑1 (MQ=255)
                                                     cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTa                                            <  1:234401/101‑1 (MQ=255)
                                                     cGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTa                                            <  2:234203/101‑1 (MQ=255)
                                                      gCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTaa                                           >  2:234760/1‑101 (MQ=255)
                                                       cTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAAc                                          >  1:234763/1‑101 (MQ=255)
                                                       cTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAAc                                          <  2:234430/101‑1 (MQ=255)
                                                        tCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAgatg                                                                          >  2:234764/1‑68 (MQ=255)
                                                        tCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAAcc                                         <  2:234419/101‑1 (MQ=255)
                                                         ccATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCg                                        <  2:234494/101‑1 (MQ=255)
                                                         ccATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCg                                        <  1:234468/101‑1 (MQ=255)
                                                          cATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGt                                       >  2:234767/1‑101 (MQ=255)
                                                               ggCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGccc                                  <  1:234521/101‑1 (MQ=255)
                                                               ggCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGccc                                  <  2:234519/101‑1 (MQ=255)
                                                                gCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCg                                 <  2:234544/101‑1 (MQ=255)
                                                                  tGTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCt                               >  1:234771/1‑101 (MQ=255)
                                                                   gTCGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCtt                              >  1:234773/1‑101 (MQ=255)
                                                                     cGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGt                            <  1:234567/101‑1 (MQ=255)
                                                                     cGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGt                            <  2:234058/101‑1 (MQ=255)
                                                                     cGCCATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGt                            <  1:234393/101‑1 (MQ=255)
                                                                       ccATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAg                          >  1:234777/1‑101 (MQ=255)
                                                                       ccATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAg                          <  1:234310/101‑1 (MQ=255)
                                                                        cATTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGc                         <  2:234338/101‑1 (MQ=255)
                                                                         aTTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAg                                                                             >  2:234782/1‑48 (MQ=255)
                                                                         aTTTTCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCa                        >  2:234778/1‑101 (MQ=255)
                                                                          ttttCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAg                       >  1:234783/1‑101 (MQ=255)
                                                                           tttCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGt                      <  1:234261/101‑1 (MQ=255)
                                                                             tCCGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGttt                    <  1:234387/101‑1 (MQ=255)
                                                                              ccGTGAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTa                   >  2:234788/1‑101 (MQ=255)
                                                                                  gAAGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCg               <  1:234455/101‑1 (MQ=255)
                                                                                   aaGTAGCGGGTAATGATCGGGAACAGCGGCTGGGACAga                                                                            >  2:234792/1‑39 (MQ=37)
                                                                                   aaGTAGCGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGa              >  1:234791/1‑101 (MQ=255)
                                                                                         cGGGTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGAttcttc        >  1:234795/1‑101 (MQ=255)
                                                                                          gggTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCa       <  1:234184/101‑1 (MQ=255)
                                                                                           ggTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCg               <  2:234596/92‑1 (MQ=255)
                                                                                            gTAATGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCaaa     <  2:234382/101‑1 (MQ=255)
                                                                                              aaTGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACa   <  1:234405/101‑1 (MQ=255)
                                                                                              aaTGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACa   <  2:234447/101‑1 (MQ=255)
                                                                                               aTGATCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAg  <  2:234444/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GCATATCGTTATGGATACGCGTATCGGTATTCTTCAGCACCTGTTCAATGATCCGCTCCATCGGGCTGTCGCCATTTTCCGTGAAGTAGCGGGTAATGGTCGGGAACAGCGGCTGGAACAGATGATATTCACACAACAGCTTATAGGTTTCGTAACCGTAGCCCGCTTGTAGCAGTTTAAGCGATTCTTCAAACAG  >  NC_000913/158196‑158391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑