Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 548,591 C→T L79L (CTG→TTG)  ylbE → DUF1116 domain‑containing protein YlbE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913548,5910CT82.2% 699.5 / 144.5 258L79L (CTG→TTG) ylbEDUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base C (18/28);  new base T (88/124);  total (106/152)
Fisher's exact test for biased strand distribution p-value = 8.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

Only 100 of 262 total aligned reads displayed.

GGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCT  >  NC_000913/548492‑548691
                                                                                                   |                                                                                                    
gggCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCt                                                                                                     <  1:809585/101‑1 (MQ=255)
gggCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCt                                                                                                     <  1:809592/101‑1 (MQ=255)
  gccgccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTgg                                                                                                   <  2:809644/101‑1 (MQ=255)
   ccgccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGc                                                                                                  >  2:810054/1‑101 (MQ=255)
     gccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCaa                                                                                                <  1:809717/101‑1 (MQ=255)
      ccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAAt                                                                                               <  2:809958/101‑1 (MQ=255)
       cAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATa                                                                                              >  2:810057/1‑101 (MQ=255)
        aaTGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATAc                                                                                             <  2:809714/101‑1 (MQ=255)
        aaTGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCGGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATAc                                                                                             >  1:810060/1‑101 (MQ=255)
             gcTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAg                                                                                        <  2:809565/101‑1 (MQ=255)
              cTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGc                                                                                       >  2:810063/1‑101 (MQ=255)
               tGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCa                                                                                      <  1:809522/101‑1 (MQ=255)
               tGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCa                                                                                      <  1:809798/101‑1 (MQ=255)
                ggCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAg                                                                                     <  2:809814/101‑1 (MQ=255)
                ggCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAg                                                                                     <  2:809602/101‑1 (MQ=255)
                 gCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAgg                                                                                    >  2:810067/1‑101 (MQ=255)
                 gCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgg                                                                                    <  2:809843/101‑1 (MQ=255)
                   aGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATGCTGGAGCAgggg                                                                                  <  2:809538/101‑1 (MQ=255)
                   aGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAgggg                                                                                  <  1:809886/101‑1 (MQ=255)
                     gagaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGa                                                                                <  2:809689/101‑1 (MQ=255)
                     gagaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGa                                                                                >  2:810071/1‑101 (MQ=255)
                       gaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAg                                                                              >  1:810075/1‑101 (MQ=255)
                        aTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGt                                                                             >  2:810077/1‑101 (MQ=255)
                        aTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGt                                                                             <  2:809914/101‑1 (MQ=255)
                         tGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTg                                                                            <  1:809776/101‑1 (MQ=255)
                          gACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGa                                                                           <  2:809490/101‑1 (MQ=255)
                            ccGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAAc                                                                         <  2:809783/101‑1 (MQ=255)
                            ccGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAAc                                                                         >  1:810079/1‑101 (MQ=255)
                              ggACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCTAAAGATGAAGCGCCGGCGTTGGCAATACTGGAGCAGGGGGAAGTGaa                                                                          >  1:810085/1‑98 (MQ=255)
                              ggACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                                       >  2:810083/1‑101 (MQ=255)
                              ggACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                                       <  1:809704/101‑1 (MQ=255)
                                aCCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa                                                                     >  2:810087/1‑101 (MQ=255)
                                  ccATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCAtt                                                                   <  2:809620/101‑1 (MQ=255)
                                  ccATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCAtt                                                                   <  1:809690/101‑1 (MQ=255)
                                   cATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTc                                                                  <  1:809646/101‑1 (MQ=255)
                                    aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGTGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc                                                                 <  1:809408/101‑1 (MQ=255)
                                      gAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                                               <  2:809797/101‑1 (MQ=255)
                                      gAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                                               <  1:809679/101‑1 (MQ=255)
                                       aaaGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTg                                                              <  1:809634/101‑1 (MQ=255)
                                        aaGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGt                                                             <  1:809543/101‑1 (MQ=255)
                                          ggggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa                                                           >  1:810092/1‑101 (MQ=255)
                                            ggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc                                                         >  2:810094/1‑101 (MQ=255)
                                             gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa                                                        <  2:809427/101‑1 (MQ=255)
                                             gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa                                                        >  1:810095/1‑101 (MQ=255)
                                              cgtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt                                                       <  2:809628/101‑1 (MQ=255)
                                               gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtg                                                      <  1:809801/101‑1 (MQ=255)
                                                  cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                                   <  2:809552/101‑1 (MQ=255)
                                                  cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                                   >  2:810099/1‑101 (MQ=255)
                                                   gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                                                  >  1:810100/1‑101 (MQ=255)
                                                    tgGGCGCATGTCTGTTCGAATGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                 <  1:809623/101‑1 (MQ=255)
                                                    tgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                                                 <  1:809468/101‑1 (MQ=255)
                                                     gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                >  1:810102/1‑101 (MQ=255)
                                                      ggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc                                               >  2:810105/1‑101 (MQ=255)
                                                         gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                                            >  1:810109/1‑101 (MQ=255)
                                                          cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                           <  1:809910/101‑1 (MQ=255)
                                                           aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg                                          >  2:810114/1‑101 (MQ=255)
                                                           aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg                                          >  1:810113/1‑101 (MQ=255)
                                                           aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg                                          <  2:809925/101‑1 (MQ=255)
                                                             gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg                                        >  2:810117/1‑101 (MQ=255)
                                                             gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg                                        <  1:809615/101‑1 (MQ=255)
                                                              tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                                       >  2:810120/1‑101 (MQ=255)
                                                               cTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                                      <  1:809823/101‑1 (MQ=255)
                                                                tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                     <  1:809747/101‑1 (MQ=255)
                                                                tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa                                     >  1:810122/1‑101 (MQ=255)
                                                                 gTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                                    <  2:809888/101‑1 (MQ=255)
                                                                 gTTCGAAGGGTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                                    >  2:810124/1‑101 (MQ=255)
                                                                   tCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg                                  >  1:810128/1‑101 (MQ=255)
                                                                   tCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg                                  <  1:809741/101‑1 (MQ=255)
                                                                    cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg                                 <  2:809833/101‑1 (MQ=255)
                                                                     gAAGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                                         <  2:809661/92‑1 (MQ=255)
                                                                     gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                >  2:810130/1‑101 (MQ=255)
                                                                       aGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATAc                                                                                             >  1:810134/1‑38 (MQ=37)
                                                                       aGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                              <  1:809668/101‑1 (MQ=255)
                                                                       aGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                              <  2:809736/101‑1 (MQ=255)
                                                                       aGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                              <  2:809711/101‑1 (MQ=255)
                                                                       aGGTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                              <  2:809648/101‑1 (MQ=255)
                                                                         gTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                            <  1:809724/101‑1 (MQ=255)
                                                                         gTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                            <  2:809669/101‑1 (MQ=255)
                                                                         gTTGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                            <  1:809790/101‑1 (MQ=255)
                                                                          ttGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa                           <  2:809702/101‑1 (MQ=255)
                                                                          ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTa                           >  1:810136/1‑101 (MQ=255)
                                                                           tGGGCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt                          >  2:810137/1‑101 (MQ=255)
                                                                            gggCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt                         <  2:809883/101‑1 (MQ=255)
                                                                            gggCGAAAGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAtt                         <  2:809580/101‑1 (MQ=255)
                                                                              gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc                       <  1:809847/101‑1 (MQ=255)
                                                                                 aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc                    >  1:810141/1‑101 (MQ=255)
                                                                                  aaGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                           >  1:810144/1‑77 (MQ=255)
                                                                                  aaGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCt                   >  2:810143/1‑101 (MQ=255)
                                                                                  aaGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCt                   <  1:809995/101‑1 (MQ=255)
                                                                                   aGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTg                  <  1:809641/101‑1 (MQ=255)
                                                                                   aGATGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTg                  <  1:809853/101‑1 (MQ=255)
                                                                                     aTGAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGcc                >  1:810148/1‑101 (MQ=255)
                                                                                       gAAGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg              >  2:810150/1‑101 (MQ=255)
                                                                                       gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg              <  2:809839/101‑1 (MQ=255)
                                                                                        aaGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCGAGt             <  2:809755/101‑1 (MQ=255)
                                                                                        aaGCGCAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGt             <  1:809564/101‑1 (MQ=255)
                                                                                          gcgcAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTAt           >  1:810154/1‑101 (MQ=255)
                                                                                           cgcAGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATg          >  2:810156/1‑101 (MQ=255)
                                                                                              aGGCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCg       >  2:810158/1‑101 (MQ=255)
                                                                                               ggCGTTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGa      >  2:810162/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
GGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCT  >  NC_000913/548492‑548691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑