BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	551,696	A→G	V300A (GTG→GCG)	purK ←	5‑(carboxyamino)imidazole ribonucleotide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	551,696	0	A	G	100.0%	1138.2 / NA	265	V300A (GTG→GCG)	purK	5‑(carboxyamino)imidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (123/142); total (123/142)

Only 100 of 265 total aligned reads displayed.

CAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATC  >  NC_000913/551599‑551793

 aGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAg                                                                                               >  1:814332/1‑101 (MQ=255)

  gCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGc                                                                                              <  1:814036/101‑1 (MQ=255)

   cGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCg                                                                                             <  2:813999/101‑1 (MQ=255)

    gTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCgg                                                                                            <  2:813891/101‑1 (MQ=255)

     tCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGc                                                                                           <  1:813968/101‑1 (MQ=255)

      cGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCa                                                                                          <  2:813937/101‑1 (MQ=255)

      cGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCa                                                                                          <  2:814194/101‑1 (MQ=255)

       gCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAg                                                                                         <  1:813961/101‑1 (MQ=255)

        cAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCa                                                                                                <  2:814044/93‑1 (MQ=255)

        cAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGt                                                                                        >  2:814335/1‑101 (MQ=255)

        cAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGt                                                                                        >  1:814336/1‑101 (MQ=255)

         aGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGtt                                                                                       >  2:814338/1‑101 (MQ=255)

          gTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGttt                                                                                      <  2:814006/101‑1 (MQ=255)

             aGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAg                                                                                   <  1:813989/101‑1 (MQ=255)

             aGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAg                                                                                   >  1:814343/1‑101 (MQ=255)

              gACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGc                                                                                  >  2:814344/1‑101 (MQ=255)

               aCGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGcc                                                                                 >  2:814347/1‑101 (MQ=255)

                cgcgATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCa                                                                                <  2:813927/101‑1 (MQ=255)

                cgcgATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCa                                                                                <  2:813832/101‑1 (MQ=255)

                 gcgATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGcc                                                                                                 >  2:814350/1‑83 (MQ=255)

                  cgATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGt                                                                              <  1:814099/101‑1 (MQ=255)

                  cgATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGt                                                                              <  2:814059/101‑1 (MQ=255)

                   gATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTc                                                                             >  1:814352/1‑101 (MQ=255)

                   gATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTc                                                                             <  2:813975/101‑1 (MQ=255)

                   gATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTc                                                                             <  1:813687/101‑1 (MQ=255)

                     tgtgTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCAt                                                                           >  1:814355/1‑101 (MQ=255)

                      gtgtCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATa                                                                          <  2:814030/101‑1 (MQ=255)

                      gtgtCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATa                                                                          <  2:814089/101‑1 (MQ=255)

                      gtgtCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATa                                                                          <  1:814214/101‑1 (MQ=255)

                      gtgtCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATa                                                                          >  2:814359/1‑101 (MQ=255)

                         tCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAAtt                                                                       <  1:814047/101‑1 (MQ=255)

                           gCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTca                                                                     >  1:814362/1‑101 (MQ=255)

                           gCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTca                                                                     >  2:814361/1‑101 (MQ=255)

                               tCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATc                                                                 >  1:814367/1‑101 (MQ=255)

                                cGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCg                                                                <  2:814226/101‑1 (MQ=255)

                                cGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCg                                                                >  2:814369/1‑101 (MQ=255)

                                  gTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCt                                                              <  2:813878/101‑1 (MQ=255)

                                  gTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCt                                                              <  1:813675/101‑1 (MQ=255)

                                    cAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTAc                                                            >  2:814373/1‑101 (MQ=255)

                                      aaTTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCa                                                          >  2:814376/1‑101 (MQ=255)

                                      aaTTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCa                                                          <  2:813921/101‑1 (MQ=255)

                                      aaTTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCa                                                          >  1:814377/1‑101 (MQ=255)

                                        ttCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGc                                                                                           >  1:814380/1‑66 (MQ=255)

                                          cAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCa                                                      <  1:813929/101‑1 (MQ=255)

                                              tGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGAtt                                                  <  2:813839/101‑1 (MQ=255)

                                                ccccACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGa                                                >  2:814384/1‑101 (MQ=255)

                                                 cccACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGat                                               <  2:814107/101‑1 (MQ=255)

                                                 cccACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGat                                               >  1:814388/1‑101 (MQ=255)

                                                  ccACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGatc                                              <  1:814077/101‑1 (MQ=255)

                                                   cACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGatca                                             <  2:814095/101‑1 (MQ=255)

                                                    aCTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGatcat                                            <  2:814257/101‑1 (MQ=255)

                                                    aCTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGatcat                                            >  1:814391/1‑101 (MQ=255)

                                                      tttACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAg                                                                               <  1:813949/64‑1 (MQ=255)

                                                      tttACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGAtcatca                                          <  1:813915/101‑1 (MQ=255)

                                                       ttACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCAc                                         <  1:814120/101‑1 (MQ=255)

                                                         aCGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCg                                       <  2:813867/101‑1 (MQ=255)

                                                         aCGCCCCGGACGGACTTCTTTGTCGTACCACTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAAtt                                                                       <  1:813824/69‑1 (MQ=255)

                                                          cGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTg                                                 >  2:814395/1‑90 (MQ=255)

                                                           gCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGAc                                     <  2:813958/101‑1 (MQ=255)

                                                           gCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGAc                                     >  1:814396/1‑101 (MQ=255)

                                                           gCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGAc                                     <  2:814064/101‑1 (MQ=255)

                                                            ccccGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAg                                                                                   <  1:813803/54‑1 (MQ=255)

                                                            ccccGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACg                                    >  1:814399/1‑101 (MQ=255)

                                                             cccGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACgg                                   <  2:813966/101‑1 (MQ=255)

                                                              ccGGACGGACTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGa                                  <  2:813954/101‑1 (MQ=255)

                                                               cggacggaCTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGat                                 >  2:814402/1‑101 (MQ=255)

                                                                ggacggacTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGatt                                >  1:814403/1‑101 (MQ=255)

                                                                     gacTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTca                           >  1:814408/1‑101 (MQ=255)

                                                                      acTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTcac                          <  2:813820/101‑1 (MQ=255)

                                                                      acTTCTTTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTcac                          >  2:814410/1‑101 (MQ=255)

                                                                        ttcttTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTcacca                        >  2:814414/1‑101 (MQ=255)

                                                                          cttTGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACt                      >  1:814415/1‑101 (MQ=255)

                                                                           tttGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTg                     >  2:814418/1‑101 (MQ=255)

                                                                             tGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGt                   <  2:814019/101‑1 (MQ=255)

                                                                             tGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGt                   >  2:814421/1‑101 (MQ=255)

                                                                             tGTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGt                   >  1:814422/1‑101 (MQ=255)

                                                                              gTCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCGCCGACGGATTATTCACCACTGGtt                  <  2:814009/101‑1 (MQ=255)

                                                                               tCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTg                 >  1:814425/1‑101 (MQ=255)

                                                                               tCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTg                 <  1:814055/101‑1 (MQ=255)

                                                                               tCGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTg                 <  1:813993/101‑1 (MQ=255)

                                                                                cGTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGc                >  2:814429/1‑101 (MQ=255)

                                                                                 gTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCg               >  1:814431/1‑101 (MQ=255)

                                                                                 gTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCg               >  1:814433/1‑101 (MQ=255)

                                                                                 gTACCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCg               <  2:813977/101‑1 (MQ=255)

                                                                                   aCCAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGt             >  2:814437/1‑101 (MQ=255)

                                                                                    ccAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTa            >  2:814440/1‑101 (MQ=255)

                                                                                     cAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTaa           <  1:814042/101‑1 (MQ=255)

                                                                                     cAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTaa           <  1:814015/101‑1 (MQ=255)

                                                                                     cAGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTaa           >  1:814441/1‑101 (MQ=255)

                                                                                      aGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAAc          <  2:813737/101‑1 (MQ=255)

                                                                                      aGTGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAAc          >  2:814444/1‑101 (MQ=255)

                                                                                        tGCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACgg        >  2:814447/1‑101 (MQ=255)

                                                                                         gCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACg         <  2:814014/99‑1 (MQ=255)

                                                                                         gCAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGc       >  1:814450/1‑101 (MQ=255)

                                                                                          cAGATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCa      >  2:814452/1‑101 (MQ=255)

                                                                                            gATGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGa    <  1:814155/101‑1 (MQ=255)

                                                                                             aTGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGAt   >  1:814455/1‑101 (MQ=255)

                                                                                              tGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATc  <  1:813874/101‑1 (MQ=255)

                                                                                              tGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATc  <  2:813912/101‑1 (MQ=255)

                                                                                              tGCGCCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATc  >  1:814458/1‑101 (MQ=255)

CAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAATTCAGATGCCCCACTTTACGCCCCGGACGGACTTCTTTGTCGTACCAGTGCAGATGCACCAGCGGCAGTTTCAGCCAGTCATAATTCACATCGCTACCAATCAGATTGATCATCACCGACGGATTATTCACCACTGGTTGCGGTAACGGCAGATC  >  NC_000913/551599‑551793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`