BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,465,729	G→A	V143V (GTG→GTA)	yfdC →	inner membrane protein YfdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,465,729	0	G	A	100.0%	1132.0 / NA	267	V143V (GTG→GTA)	yfdC	inner membrane protein YfdC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (110/157); total (110/157)

Only 100 of 278 total aligned reads displayed.

CAATTATTTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTGCTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCAAAATCG  >  NC_000913/2465631‑2465826

cAATTATTTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGtact                                                                                                 <  2:3972982/101‑1 (MQ=255)

 aaTTATTTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGtactg                                                                                                >  1:3973395/1‑101 (MQ=255)

 aaTTATTTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGtactg                                                                                                <  2:3973082/101‑1 (MQ=255)

   ttattTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGtact                                                                                                 <  1:3972974/98‑1 (MQ=255)

    tattTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTActgctg                                                                                             <  1:3973192/101‑1 (MQ=255)

    tattTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTActgctg                                                                                             >  1:3973399/1‑101 (MQ=255)

     attTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTgg                                                                                            <  1:3973155/101‑1 (MQ=255)

     attTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTgg                                                                                            >  2:3973401/1‑101 (MQ=255)

       ttACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGt                                                                                          <  2:3973248/101‑1 (MQ=255)

        tACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTa                                                                                         <  2:3973221/101‑1 (MQ=255)

        tACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTa                                                                                         >  2:3973405/1‑101 (MQ=255)

        tACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTa                                                                                         <  2:3973003/101‑1 (MQ=255)

        tACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTa                                                                                         <  1:3972792/101‑1 (MQ=255)

         aCCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTaa                                                                                        <  1:3972856/101‑1 (MQ=255)

            gAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAatat                                                                                     <  2:3972860/101‑1 (MQ=255)

            gAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAatat                                                                                     >  2:3973408/1‑101 (MQ=255)

             aaaaTACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATAtt                                                                                    >  1:3973409/1‑101 (MQ=255)

              aaaTACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATtc                                                                                   <  1:3972912/101‑1 (MQ=255)

               aaTACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATtct                                                                                  <  2:3973013/101‑1 (MQ=255)

                aTACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATtctc                                                                                 <  1:3973055/101‑1 (MQ=255)

                aTACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATtctc                                                                                 <  1:3972686/101‑1 (MQ=255)

                 tACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCg                                                                                >  1:3973413/1‑101 (MQ=255)

                 tACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCg                                                                                <  2:3972969/101‑1 (MQ=255)

                  aCCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCgg                                                                               >  2:3973416/1‑101 (MQ=255)

                   ccGTGACTGCGGTACTCCCCGTCATGCAAAACCCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCg                                                                                <  1:3973059/99‑1 (MQ=255)

                   ccGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCggg                                                                              <  2:3973053/101‑1 (MQ=255)

                   ccGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCggg                                                                              <  1:3972991/101‑1 (MQ=255)

                   ccGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCggg                                                                              >  1:3973417/1‑101 (MQ=255)

                    cGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGa                                                                             <  1:3972943/101‑1 (MQ=255)

                     gTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGAc                                                                            >  2:3973421/1‑101 (MQ=255)

                      tGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACa                                                                           <  2:3973125/101‑1 (MQ=255)

                       gACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAg                                                                          >  1:3973424/1‑101 (MQ=255)

                          tGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTa                                                                       >  2:3973428/1‑101 (MQ=255)

                           gCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTAt                                                                      >  1:3973430/1‑101 (MQ=255)

                            cGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTAtt                                                                     <  2:3973022/101‑1 (MQ=255)

                             ggTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTg                                                                    <  2:3973095/101‑1 (MQ=255)

                              gTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTgc                                                                   <  2:3973142/101‑1 (MQ=255)

                                actacCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTgcgg                                                                 <  1:3972954/101‑1 (MQ=255)

                                 ctacCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTgcggc                                                                <  2:3973018/101‑1 (MQ=255)

                                  tacCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTgcggcg                                                               <  1:3973110/101‑1 (MQ=255)

                                   acCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGt                                                              >  1:3973434/1‑101 (MQ=255)

                                    cccGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAg                                                                          <  1:3973198/88‑1 (MQ=255)

                                    cccGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTg                                                             <  2:3973106/101‑1 (MQ=255)

                                    cccGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTg                                                             <  2:3972904/101‑1 (MQ=255)

                                    cccGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTg                                                             >  1:3973436/1‑101 (MQ=255)

                                     ccGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTgcggcg                                                               <  2:3973041/98‑1 (MQ=255)

                                     ccGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTgg                                                            >  2:3973438/1‑101 (MQ=255)

                                      cGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTggg                                                           <  1:3972967/101‑1 (MQ=255)

                                      cGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTggg                                                           <  2:3972767/101‑1 (MQ=255)

                                        tCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCa                                                         <  1:3973089/101‑1 (MQ=255)

                                           tGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCAttt                                                      >  1:3973442/1‑101 (MQ=255)

                                           tGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCAttt                                                      <  2:3972935/101‑1 (MQ=255)

                                           tGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCAttt                                                      >  2:3973441/1‑101 (MQ=255)

                                             cAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGa                                                    >  1:3973446/1‑101 (MQ=255)

                                              aaaaaCCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGaa                                                   >  2:3973448/1‑101 (MQ=255)

                                                    cGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATg                                             <  1:3973266/101‑1 (MQ=255)

                                                     gACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGc                                            >  1:3973451/1‑101 (MQ=255)

                                                      aCAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGcc                                           <  1:3973029/101‑1 (MQ=255)

                                                       cAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCt                                          <  1:3973354/101‑1 (MQ=255)

                                                        aaTGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTa                                         >  2:3973455/1‑101 (MQ=255)

                                                         aTGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTAt                                        <  1:3973213/101‑1 (MQ=255)

                                                          tGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATc                                       >  2:3973458/1‑101 (MQ=255)

                                                           gAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCt                                      <  1:3973071/101‑1 (MQ=255)

                                                           gAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCt                                      <  1:3973008/101‑1 (MQ=255)

                                                           gAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCt                                      <  1:3973180/101‑1 (MQ=255)

                                                            aGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCtt                                     <  2:3973069/101‑1 (MQ=255)

                                                              cAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCa                                   >  1:3973460/1‑101 (MQ=255)

                                                              cAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCa                                   <  1:3973118/101‑1 (MQ=255)

                                                                aCGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAAt                                 >  2:3973463/1‑101 (MQ=255)

                                                                 cGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATg                                <  1:3973127/101‑1 (MQ=255)

                                                                 cGTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATg                                <  1:3973302/101‑1 (MQ=255)

                                                                  gTCGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATga                               >  1:3973464/1‑101 (MQ=255)

                                                                    cGGCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATgaag                             <  2:3973205/101‑1 (MQ=255)

                                                                     ggCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATgaaga                            <  1:3973100/101‑1 (MQ=255)

                                                                      gCTTACTTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATgaagaa                           <  1:3973044/101‑1 (MQ=255)

                                                                      gCTTACCTATACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATgaagaa                           >  2:3973469/1‑101 (MQ=255)

                                                                       cttacttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGaaa                          <  1:3973036/101‑1 (MQ=255)

                                                                       cttacttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGaaa                          >  2:3973473/1‑101 (MQ=255)

                                                                       cttacttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGaaa                          >  1:3973472/1‑101 (MQ=255)

                                                                       cttacttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGaaa                          <  1:3973175/101‑1 (MQ=255)

                                                                         tacttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACt                        <  2:3973183/101‑1 (MQ=255)

                                                                         tacttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACt                        <  2:3972790/101‑1 (MQ=255)

                                                                         tacttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACt                        <  2:3973384/101‑1 (MQ=255)

                                                                          acttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTc                       <  1:3973148/101‑1 (MQ=255)

                                                                           cttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTcg                      <  2:3972956/101‑1 (MQ=255)

                                                                            ttaTACGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTcgc                     >  2:3973478/1‑101 (MQ=255)

                                                                             tataCGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTcgcg                    >  1:3973479/1‑101 (MQ=255)

                                                                               taCGGTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGAt                  >  2:3973483/1‑101 (MQ=255)

                                                                                  ggTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCa               <  2:3972977/101‑1 (MQ=255)

                                                                                  ggTTATGGGGCGTCGTACTGCTGGGAAATATTCTCGGGACAGGTATTGCGGCGTGGGCAtt                                                       >  2:3973486/1‑61 (MQ=255)

                                                                                   gTTATGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCAt              >  1:3973487/1‑101 (MQ=255)

                                                                                      aTGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTg           >  1:3973493/1‑101 (MQ=255)

                                                                                      aTGGGGCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTg           >  2:3973492/1‑101 (MQ=255)

                                                                                         gggCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCa        <  2:3973031/101‑1 (MQ=255)

                                                                                         gggCGTCGTACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCa        >  1:3973497/1‑101 (MQ=255)

                                                                                          ggCGTCATACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCaa       >  2:3973498/1‑101 (MQ=255)

                                                                                            cgtcgtACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCaaaa     <  1:3973162/101‑1 (MQ=255)

                                                                                             gtcgtACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCAAAAt    >  1:3973503/1‑101 (MQ=255)

                                                                                             gtcgtACTGCTGGGTAATATTCTCGGGACAGGGATTGCGGGGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCAAAAt    >  2:3973506/1‑101 (MQ=255)

                                                                                               cgtACTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCAAAATCg  <  1:3972926/101‑1 (MQ=255)

CAATTATTTACCGAAAATACCGTGACTGCGGTACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTGCTGCTGGGTAATATTCTCGGGACAGGTATTGCGGCGTGGGCATTTGAATATATGCCTATCTTCAATGAAGAAACTCGCGATGCATTTGTCAAAATCG  >  NC_000913/2465631‑2465826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`