BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,613,439	G→A	E514K (GAA→AAA)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,613,439	0	G	A	100.0%	999.6 / NA	235	E514K (GAA→AAA)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (118/117); total (118/117)

Only 100 of 237 total aligned reads displayed.

TCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGC  >  NC_000913/2613341‑2613536

tCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGaaa                                                                                                 >  1:4187020/1‑101 (MQ=255)

 cAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAAt                                                                                                <  1:4186751/101‑1 (MQ=255)

  agagTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAAtt                                                                                               >  1:4187023/1‑101 (MQ=255)

   gagTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAAtt                                                                                               >  1:4187025/1‑100 (MQ=255)

   gagTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTg                                                                                              <  2:4186868/101‑1 (MQ=255)

   gagTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTg                                                                                              <  2:4186609/101‑1 (MQ=255)

     gTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTgcc                                                                                            <  2:4186688/101‑1 (MQ=255)

     gTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTgcc                                                                                            >  2:4187026/1‑101 (MQ=255)

       cATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTgccgc                                                                                          <  1:4186720/101‑1 (MQ=255)

        aTTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTgccgcc                                                                                         >  2:4187029/1‑101 (MQ=255)

        aTTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTgccgcc                                                                                         >  1:4187030/1‑101 (MQ=255)

             cgccACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTAc                                                                                    >  1:4187033/1‑101 (MQ=255)

               ccaccaACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTAcgc                                                                                  >  2:4187038/1‑101 (MQ=255)

               ccaccaACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTAcgc                                                                                  >  1:4187037/1‑101 (MQ=255)

                  ccaACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGAc                                                                               <  2:4186719/101‑1 (MQ=255)

                   caACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGAcc                                                                              >  1:4187041/1‑101 (MQ=255)

                     aCCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGt                                                                            <  2:4186678/101‑1 (MQ=255)

                      ccGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTc                                                                           >  2:4187046/1‑101 (MQ=255)

                      ccGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTc                                                                           <  2:4186799/101‑1 (MQ=255)

                      ccGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTc                                                                           <  1:4186618/101‑1 (MQ=255)

                      ccGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTc                                                                           >  1:4187044/1‑101 (MQ=255)

                      ccGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTc                                                                           <  2:4186579/101‑1 (MQ=255)

                        gTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCg                                                                         >  2:4187049/1‑101 (MQ=255)

                          gATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGa                                                                       >  1:4187050/1‑101 (MQ=255)

                           aTTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCCCTGAAATTGCCGCCGCTACGCGACCGTCCGGaa                                                                      <  2:4186797/101‑1 (MQ=255)

                            tttGTGGCAAATGGTTGAAGATCGCCAGTTTCTCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAg                                                                     <  2:4186585/101‑1 (MQ=255)

                            tttGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAg                                                                     <  2:4186653/101‑1 (MQ=255)

                            tttGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAg                                                                     <  2:4186930/101‑1 (MQ=255)

                             ttGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGa                                                                    >  2:4187054/1‑101 (MQ=255)

                               gtgGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGata                                                                  >  2:4187056/1‑101 (MQ=255)

                                tgGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGatat                                                                 <  1:4186564/101‑1 (MQ=255)

                                tgGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGatat                                                                 <  1:4186660/101‑1 (MQ=255)

                                 ggCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATc                                                                >  1:4187057/1‑101 (MQ=255)

                                 ggCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATc                                                                <  1:4186645/101‑1 (MQ=255)

                                  gCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATcc                                                               <  1:4186837/101‑1 (MQ=255)

                                   cAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATccc                                                              <  2:4186695/101‑1 (MQ=255)

                                   cAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATccc                                                              <  2:4186704/101‑1 (MQ=255)

                                    aaaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCct                                                             >  1:4187062/1‑101 (MQ=255)

                                    aaaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCct                                                             >  2:4187060/1‑101 (MQ=255)

                                     aaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCctc                                                            >  1:4187064/1‑101 (MQ=255)

                                      aTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCctct                                                           <  2:4186822/101‑1 (MQ=255)

                                      aTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCctct                                                           <  1:4186773/101‑1 (MQ=255)

                                      aTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCctct                                                           <  2:4186739/101‑1 (MQ=255)

                                       tGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCtt                                                          >  1:4187068/1‑101 (MQ=255)

                                       tGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCtt                                                          <  2:4186665/101‑1 (MQ=255)

                                       tGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCtt                                                          <  2:4186960/101‑1 (MQ=255)

                                         gTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCtttt                                                        >  1:4187070/1‑101 (MQ=255)

                                         gTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCtttt                                                        <  2:4186785/101‑1 (MQ=255)

                                          ttGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTa                                                       <  1:4186541/101‑1 (MQ=255)

                                          ttGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTa                                                       <  1:4186628/101‑1 (MQ=255)

                                            gAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGc                                                     >  1:4187072/1‑101 (MQ=255)

                                               gATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCaaa                                                  <  1:4186789/101‑1 (MQ=255)

                                               gATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCaaa                                                  <  1:4186668/101‑1 (MQ=255)

                                                aTCGCCAGTTTCGCAGCGATATCTTTTATCGCCTGAATGTCTTCCCACTGAAATTgccg                                                                                           >  2:4187073/1‑59 (MQ=255)

                                                 tCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGat                                                                   <  2:4186706/82‑1 (MQ=255)

                                                     cAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACAttt                                            <  2:4186831/101‑1 (MQ=255)

                                                     cAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACAttt                                            >  1:4187077/1‑101 (MQ=255)

                                                     cAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACAttt                                            >  2:4187078/1‑101 (MQ=255)

                                                      attttCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTc                                           >  1:4187083/3‑101 (MQ=255)

                                                      aGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTcc                                                                          <  2:4186752/70‑1 (MQ=255)

                                                      aGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTc                                           >  1:4187080/1‑101 (MQ=255)

                                                       gTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGAcc                                                                              >  1:4187087/1‑65 (MQ=255)

                                                         ttCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACg                                        >  2:4187088/1‑101 (MQ=255)

                                                          tCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGc                                       <  2:4186770/101‑1 (MQ=255)

                                                            gCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaa                                     >  2:4187090/1‑101 (MQ=255)

                                                            gCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaa                                     <  1:4186745/101‑1 (MQ=255)

                                                             cAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaaa                                    >  2:4187093/1‑101 (MQ=255)

                                                              aGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaaaa                                   >  2:4187096/1‑101 (MQ=255)

                                                               gCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCtttt                                                        <  2:4186716/79‑1 (MQ=255)

                                                               gCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaaaaa                                  <  1:4186700/101‑1 (MQ=255)

                                                               gCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaaaaa                                  <  1:4186793/101‑1 (MQ=255)

                                                               gCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaaaaa                                  >  1:4187097/1‑101 (MQ=255)

                                                                cGATCTCTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaaaaaa                                 >  2:4187099/1‑101 (MQ=255)

                                                                   tctcTTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATgg                              >  1:4187104/1‑101 (MQ=255)

                                                                    ctctTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTc                                                                           >  2:4187106/1‑55 (MQ=255)

                                                                    ctctTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGgc                             <  1:4186931/101‑1 (MQ=255)

                                                                      ctTTTATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGgcgc                           >  1:4187108/1‑101 (MQ=255)

                                                                       ttttATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGgcgcg                          >  1:4187110/1‑101 (MQ=255)

                                                                         ttATCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGcc                        >  2:4187112/1‑101 (MQ=255)

                                                                           aTCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGat                                                                   <  2:4186641/56‑1 (MQ=255)

                                                                           aTCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCat                      >  1:4187114/1‑101 (MQ=255)

                                                                            tCGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCata                     <  1:4186811/101‑1 (MQ=255)

                                                                             cGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCg                                                                             >  2:4187118/1‑44 (MQ=255)

                                                                             cGCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCatat                    >  1:4187116/1‑101 (MQ=255)

                                                                              gCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATg                   >  1:4187121/1‑101 (MQ=255)

                                                                              gCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATg                   <  2:4186863/101‑1 (MQ=255)

                                                                              gCCTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATg                   <  1:4186169/101‑1 (MQ=255)

                                                                                cTGAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGaa                 <  2:4186892/101‑1 (MQ=255)

                                                                                  gAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATc               <  1:4186897/101‑1 (MQ=255)

                                                                                  gAATGTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATc               >  2:4187125/1‑101 (MQ=255)

                                                                                      gTCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCgcgc           <  2:4186848/101‑1 (MQ=255)

                                                                                       tCTTCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCa          >  1:4187126/1‑101 (MQ=255)

                                                                                          tCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAAtt       >  2:4187130/1‑101 (MQ=255)

                                                                                          tCCCACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAAtt       >  1:4187129/1‑101 (MQ=255)

                                                                                            ccACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGa     >  1:4187133/1‑101 (MQ=255)

                                                                                             cACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCaaaaa                                  <  1:4186730/71‑1 (MQ=255)

                                                                                             cACTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGAc    <  1:4186873/101‑1 (MQ=255)

                                                                                              aCTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACg   <  2:4186845/101‑1 (MQ=255)

                                                                                               cTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGc  >  1:4187139/1‑101 (MQ=255)

                                                                                               cTGAAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGc  >  1:4187137/1‑101 (MQ=255)

TCAGAGTCATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGC  >  NC_000913/2613341‑2613536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`