| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,931,803 | T→C | V250A (GTA→GCA) | ygdG → | flap endonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,931,803 | 0 | T | C | 99.7% | 1189.0 / NA | 282 | V250A (GTA→GCA) | ygdG | flap endonuclease |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (137/144); total (137/145) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 282 total aligned reads displayed.
ATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTC > NC_000913/2931705‑2931900 | aTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCAc > 1:4649391/1‑101 (MQ=255) tAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACgg > 2:4649394/1‑101 (MQ=255) aGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTCGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGt > 1:4649396/1‑101 (MQ=255) aaaCCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTaa < 1:4649145/101‑1 (MQ=255) aaaCCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTaa > 1:4649400/1‑101 (MQ=255) cccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACgg > 2:4649405/1‑101 (MQ=255) cccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACgg > 1:4649404/1‑101 (MQ=255) ccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGc > 2:4649408/1‑101 (MQ=255) cATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCg > 1:4649411/1‑101 (MQ=255) aTAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa < 1:4649024/101‑1 (MQ=255) aTAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649415/1‑101 (MQ=255) tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649417/1‑100 (MQ=255) tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAg < 2:4649207/101‑1 (MQ=255) tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAg < 1:4649080/101‑1 (MQ=255) agagATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649422/1‑97 (MQ=255) agagATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649421/1‑97 (MQ=255) agaTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649427/1‑95 (MQ=255) gaTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAAc > 1:4649432/1‑89 (MQ=255) gaTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649430/1‑94 (MQ=255) tGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATAc < 2:4648912/101‑1 (MQ=255) gCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATAcgc < 1:4648643/101‑1 (MQ=255) tttCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649437/1‑87 (MQ=255) ttCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649438/1‑86 (MQ=255) tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649442/1‑83 (MQ=255) tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAAc < 2:4649044/101‑1 (MQ=255) tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAAc < 1:4648874/101‑1 (MQ=255) tgtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAAc < 1:4648938/101‑1 (MQ=255) gtgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAAcg < 2:4649164/101‑1 (MQ=255) tgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCg > 2:4649445/1‑80 (MQ=255) tgCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAAcgt < 2:4648898/101‑1 (MQ=255) gCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649448/1‑80 (MQ=255) ccGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649450/1‑79 (MQ=255) cgcgATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649454/1‑78 (MQ=255) gcgATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649457/1‑77 (MQ=255) gcgATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTa < 1:4648923/101‑1 (MQ=255) gATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAg > 1:4649460/1‑76 (MQ=255) gATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATc < 2:4648997/101‑1 (MQ=255) tatTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649463/1‑73 (MQ=255) tatTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCg < 1:4648890/101‑1 (MQ=255) atTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649465/1‑72 (MQ=255) tGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCg > 1:4649468/1‑69 (MQ=255) gCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCAt < 1:4649033/101‑1 (MQ=255) cccGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649472/1‑68 (MQ=255) cccGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649473/1‑68 (MQ=255) cGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649477/1‑66 (MQ=255) cGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTAt < 1:4649133/101‑1 (MQ=255) cGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTAt < 1:4649140/101‑1 (MQ=255) gCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649478/1‑65 (MQ=255) gCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649483/1‑65 (MQ=255) cTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATca < 2:4649003/101‑1 (MQ=255) ttACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649486/1‑63 (MQ=255) ttACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATcac < 2:4649180/101‑1 (MQ=255) tACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACgg > 2:4649492/1‑59 (MQ=255) tACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCg > 1:4649491/1‑61 (MQ=255) tACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649488/1‑62 (MQ=255) tACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATcaca < 2:4648975/101‑1 (MQ=255) aaaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCa < 2:4649248/101‑1 (MQ=255) aaCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649496/1‑58 (MQ=255) aCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649501/1‑57 (MQ=255) aCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649500/1‑57 (MQ=255) ccGATTTTCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTAt < 1:4649077/91‑1 (MQ=255) ccGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAgcg < 2:4648803/101‑1 (MQ=255) gATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649506/1‑54 (MQ=255) gATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCa < 2:4649015/101‑1 (MQ=255) aTTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649507/1‑53 (MQ=255) aTTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAt < 2:4648892/101‑1 (MQ=255) ttGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt < 1:4649111/101‑1 (MQ=255) tGCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTa < 1:4649334/101‑1 (MQ=255) gCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCa < 1:4648958/64‑1 (MQ=255) gCATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAc < 1:4648969/101‑1 (MQ=255) cATATCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGc > 2:4649511/1‑46 (MQ=255) atatCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649514/1‑47 (MQ=255) atatCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCa < 2:4649056/101‑1 (MQ=255) tatCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649515/1‑46 (MQ=255) tCGACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGc < 2:4649064/101‑1 (MQ=255) gACGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649520/1‑42 (MQ=255) aCGGCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGt < 1:4649095/101‑1 (MQ=255) ggCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649523/1‑39 (MQ=39) ggCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTAt < 2:4648866/101‑1 (MQ=255) gCAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATg < 2:4649029/101‑1 (MQ=255) cAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649525/1‑37 (MQ=38) cAATTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATgg < 1:4648941/101‑1 (MQ=255) aaTTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 2:4649529/1‑36 (MQ=38) aaTTTACAGCAATTGCGGTTGGCACGGTAACGGCGa > 1:4649527/1‑36 (MQ=38) aaTTTACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGt < 1:4649156/101‑1 (MQ=255) tttACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTaa < 1:4648814/101‑1 (MQ=255) ttACAGCAATTGCGGTTGGCGCGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTaaa < 1:4648988/101‑1 (MQ=255) ttACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTa < 1:4649012/84‑1 (MQ=255) ttACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTaaa < 2:4649104/101‑1 (MQ=255) tACAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAg < 1:4649057/101‑1 (MQ=255) cAGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGct < 2:4648836/101‑1 (MQ=255) aGCAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGctc < 1:4649120/101‑1 (MQ=255) cAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTa < 1:4649185/101‑1 (MQ=255) cAATTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTa < 2:4649189/101‑1 (MQ=255) aTTGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACa < 2:4649086/101‑1 (MQ=255) tGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAat < 2:4648925/101‑1 (MQ=255) tGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAat < 2:4648952/101‑1 (MQ=255) tGCGGTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAat < 1:4649004/101‑1 (MQ=255) gTTGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATca < 1:4649046/56‑1 (MQ=255) tGGCACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTc < 1:4649269/101‑1 (MQ=255) | ATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTC > NC_000913/2931705‑2931900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |