Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,562,313 A→G G121G (GGT→GGC)  yjiJ ← putative transporter YjiJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,562,3130AG99.6% 1173.9 / ‑4.3 280G121G (GGT→GGC) yjiJputative transporter YjiJ
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (160/119);  total (161/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

Only 100 of 280 total aligned reads displayed.

CCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGG  >  NC_000913/4562215‑4562410
                                                                                                  |                                                                                                 
cccAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGcc                                                                                                 >  1:7119417/1‑101 (MQ=255)
cccAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGcc                                                                                                 >  2:7119418/1‑101 (MQ=255)
 ccAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCg                                                                                                >  1:7119423/1‑101 (MQ=255)
 ccAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCg                                                                                                >  2:7119422/1‑101 (MQ=255)
    gTGCGATGCCAATGCTAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGgcg                                                                                             <  2:7118958/101‑1 (MQ=255)
    gTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGgcg                                                                                             <  1:7118944/101‑1 (MQ=255)
     tGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGgcgc                                                                                            >  1:7119427/1‑101 (MQ=255)
     tGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGgcgc                                                                                            <  2:7119039/101‑1 (MQ=255)
      gCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCt                                                                                           >  2:7119429/1‑101 (MQ=255)
      gCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCt                                                                                           <  1:7119087/101‑1 (MQ=255)
       cGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTg                                                                                          <  2:7118898/101‑1 (MQ=255)
        gATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTgg                                                                                         >  1:7119432/1‑101 (MQ=255)
         atgccaatgccaACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGc                                                                                        >  2:7119436/1‑101 (MQ=255)
         atgccaatgccaACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGc                                                                                        <  2:7119021/101‑1 (MQ=255)
          tgccaatgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCg                                                                                       <  2:7119093/101‑1 (MQ=255)
          tgccaatgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCg                                                                                       >  1:7119441/1‑101 (MQ=255)
             caatgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTgg                                                                                         >  2:7119445/1‑96 (MQ=255)
              aatgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGacac                                                                                   >  1:7119447/1‑101 (MQ=255)
               atgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACAcc                                                                                  <  1:7119001/101‑1 (MQ=255)
               atgccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACAcc                                                                                  >  2:7119450/1‑101 (MQ=255)
                 gccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCg                                                                                <  2:7119004/101‑1 (MQ=255)
                 gccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCg                                                                                <  2:7118875/101‑1 (MQ=255)
                 gccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCg                                                                                <  2:7119316/101‑1 (MQ=255)
                  ccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGc                                                                               >  1:7119455/1‑101 (MQ=255)
                  ccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGc                                                                               >  2:7119454/1‑101 (MQ=255)
                  ccaaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGc                                                                               <  1:7118902/101‑1 (MQ=255)
                    aaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGcc                                                                              >  2:7119462/1‑100 (MQ=255)
                    aaCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCa                                                                             >  2:7119458/1‑101 (MQ=255)
                     aCGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAg                                                                            <  2:7119193/101‑1 (MQ=255)
                      cGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCg                                                                                                <  2:7119052/80‑1 (MQ=255)
                      cGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAgg                                                                           <  2:7119032/101‑1 (MQ=255)
                      cGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAgg                                                                           <  1:7118766/101‑1 (MQ=255)
                        ccAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGAc                                                                         >  2:7119466/1‑101 (MQ=255)
                         cAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGAcg                                                                        >  1:7119468/1‑101 (MQ=255)
                         cAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGAcg                                                                        <  1:7118976/101‑1 (MQ=255)
                          aGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGAcgc                                                                       <  1:7119208/101‑1 (MQ=255)
                          aGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGAcgc                                                                       <  1:7118830/101‑1 (MQ=255)
                           gAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACCCCCGCCAGGAcgcg                                                                      <  2:7118969/101‑1 (MQ=255)
                             aaaCAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGaa                                                                    <  2:7119128/101‑1 (MQ=255)
                              aaCAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAAt                                                                   >  1:7119473/1‑101 (MQ=255)
                               aCAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATc                                                                  <  1:7119068/101‑1 (MQ=255)
                                cAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCa                                                                 >  1:7119478/1‑101 (MQ=255)
                                 aaaGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCaa                                                                >  2:7119479/1‑101 (MQ=255)
                                  aaGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAAt                                                               >  2:7119482/1‑101 (MQ=255)
                                      tGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAaca                                                           >  2:7119488/1‑101 (MQ=255)
                                      tGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAaca                                                           >  1:7119486/1‑101 (MQ=255)
                                        ccAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACAcc                                                         >  1:7119490/1‑101 (MQ=255)
                                          aGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAg                                                       <  2:7119073/101‑1 (MQ=255)
                                             acaAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAAt                                                    >  1:7119494/1‑101 (MQ=255)
                                              caAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATa                                                   >  2:7119497/1‑101 (MQ=255)
                                               aaaaGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATaa                                                  >  2:7119500/1‑101 (MQ=255)
                                                 aaGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAAt                                                >  1:7119503/1‑101 (MQ=255)
                                                   ggATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATgg                                              >  1:7119508/1‑101 (MQ=255)
                                                    gATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggc                                             <  1:7119036/101‑1 (MQ=255)
                                                    gATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggc                                             <  2:7119189/101‑1 (MQ=255)
                                                     aTGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggcg                                            <  2:7118808/101‑1 (MQ=255)
                                                     aTGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggcg                                            >  2:7119511/1‑101 (MQ=255)
                                                      tGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggcgg                                           >  1:7119512/1‑101 (MQ=255)
                                                          gcgTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAAc                                       >  2:7119516/1‑101 (MQ=255)
                                                           cgTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAgg                                                                           >  1:7119518/1‑64 (MQ=255)
                                                             tgtgCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggcg                                            <  2:7119105/93‑1 (MQ=255)
                                                             tgtgCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCAt                                    <  1:7119466/101‑1 (MQ=255)
                                                              gtgCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATg                                   >  2:7119521/1‑101 (MQ=255)
                                                              gtgCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATg                                   >  1:7119522/1‑101 (MQ=255)
                                                              gtgCTGCATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATg                                   <  1:7118683/101‑1 (MQ=255)
                                                                gctgcATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGcc                                 >  1:7119525/1‑101 (MQ=255)
                                                                 ctgcATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCa                                <  2:7119291/101‑1 (MQ=255)
                                                                  tgcATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCAt                               <  2:7118776/101‑1 (MQ=255)
                                                                   gcATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCAtt                              >  1:7119528/1‑101 (MQ=255)
                                                                    cATAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTg                             <  2:7119170/101‑1 (MQ=255)
                                                                     aTAATCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGc                            <  2:7119234/101‑1 (MQ=255)
                                                                        aTCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAg                         <  1:7119272/101‑1 (MQ=255)
                                                                        aTCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAg                         >  2:7119533/1‑101 (MQ=255)
                                                                        aTCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAg                         >  1:7119534/1‑101 (MQ=255)
                                                                         tCAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAgg                        >  2:7119538/1‑101 (MQ=255)
                                                                          cAGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGa                       >  1:7119541/1‑101 (MQ=255)
                                                                           aGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggc                                             >  2:7119545/1‑78 (MQ=255)
                                                                           aGCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGAt                      >  2:7119543/1‑101 (MQ=255)
                                                                            gCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATc                     <  1:7119154/101‑1 (MQ=255)
                                                                            gCGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATc                     <  1:7119315/101‑1 (MQ=255)
                                                                             cGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATca                    >  1:7119549/1‑101 (MQ=255)
                                                                             cGTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATca                    >  2:7119550/1‑101 (MQ=255)
                                                                              gTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAgg                                                                           >  2:7119553/1‑45 (MQ=255)
                                                                              gTCGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATcaa                   <  2:7119103/101‑1 (MQ=255)
                                                                                cGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATcaaca                 >  2:7119557/1‑101 (MQ=255)
                                                                                cGAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATcaaca                 <  2:7119134/101‑1 (MQ=255)
                                                                                 gAACCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATcaacaa                >  1:7119561/1‑101 (MQ=255)
                                                                                  aaCCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCaacaac               >  2:7119563/1‑101 (MQ=255)
                                                                                   aCCAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATggc                                             >  2:7119565/1‑70 (MQ=255)
                                                                                    ccAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAAccc             <  1:7118966/101‑1 (MQ=255)
                                                                                     cAAAAATCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCg            <  1:7119094/101‑1 (MQ=255)
                                                                                      aaaaaTCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGc           >  1:7119571/1‑101 (MQ=255)
                                                                                      aaaaaTCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGc           >  2:7119569/1‑101 (MQ=255)
                                                                                        aaaTCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTc         <  1:7119179/101‑1 (MQ=255)
                                                                                         aaTCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCg        <  2:7118992/101‑1 (MQ=255)
                                                                                         aaTCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCg        >  2:7119574/1‑101 (MQ=255)
                                                                                           tCAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGcc      >  1:7119577/1‑101 (MQ=255)
                                                                                            cAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCa     >  2:7119579/1‑101 (MQ=255)
                                                                                            cAGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCa     <  1:7119225/101‑1 (MQ=255)
                                                                                             aGCATGCCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAg    >  1:7119582/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCCAGTGCGATGCCAATGCCAACGCCAGAAAACAAAGCTGCCAGCACAAAAGGATGGCGCGTGTGCTGCATAATCAGCGTCGAACCAAAAATCAGCATACCGGCGCTGGCGACACCCGCCAGGACGCGAATCAATAACACCAGAATAAATGGCGGCAACCATGCCATTGCGAGGATCAACAACCCGCTCGCCAGGG  >  NC_000913/4562215‑4562410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑