BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,594,249	C→T	W158* (TGG→TGA)	lgoT ←	galactonate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,594,249	0	C	T	100.0%	1214.4 / NA	284	W158* (TGG→TGA)	lgoT	galactonate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (125/159); total (125/159)

Only 100 of 284 total aligned reads displayed.

AGCATCATCGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAACCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCA  >  NC_000913/4594151‑4594346

  catcatCGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGt                                                                                               >  1:7169161/1‑101 (MQ=255)

  catcatCGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGt                                                                                               <  1:7168720/101‑1 (MQ=255)

    tcatcGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCg                                                                                             >  2:7169165/1‑101 (MQ=255)

     catcGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGt                                                                                            <  2:7168685/101‑1 (MQ=255)

       tcGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCg                                                                                             <  2:7168709/98‑1 (MQ=255)

       tcGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTa                                                                                          >  2:7169168/1‑101 (MQ=255)

        cgccgcCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTaa                                                                                         >  1:7169169/1‑101 (MQ=255)

         gccgccAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAAt                                                                                        <  1:7168442/101‑1 (MQ=255)

         gccgccAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAAt                                                                                        <  1:7168696/101‑1 (MQ=255)

           cgccAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAg                                                                                                <  2:7168870/91‑1 (MQ=255)

           cgccAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGa                                                                                      >  2:7169173/1‑101 (MQ=255)

           cgccAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGa                                                                                      >  1:7169175/1‑101 (MQ=255)

             ccAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACt                                                                                    <  2:7168754/101‑1 (MQ=255)

              cAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACtt                                                                                   <  1:7168892/101‑1 (MQ=255)

                gAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACtttt                                                                                 <  2:7168888/101‑1 (MQ=255)

                gAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACttt                                                                                  >  2:7169180/1‑100 (MQ=255)

                  aTCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTac                                                                               >  2:7169183/1‑101 (MQ=255)

                  aTCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTac                                                                               >  1:7169182/1‑101 (MQ=255)

                  aTCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTac                                                                               <  2:7168843/101‑1 (MQ=255)

                   tCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTaca                                                                              <  1:7168743/101‑1 (MQ=255)

                   tCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTaca                                                                              <  2:7168820/101‑1 (MQ=255)

                     ggTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACAcc                                                                            <  2:7168811/101‑1 (MQ=255)

                       tGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGc                                                                          <  1:7168831/101‑1 (MQ=255)

                         ggCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCAt                                                                        <  2:7168777/101‑1 (MQ=255)

                          gCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATg                                                                       <  1:7168546/101‑1 (MQ=255)

                           cTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGtt                                                                                           <  1:7168845/80‑1 (MQ=255)

                             tACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGt                                                                    <  1:7168900/101‑1 (MQ=255)

                              aCGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGtt                                                                   <  1:7168868/101‑1 (MQ=255)

                              aCGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGtt                                                                   <  1:7168978/101‑1 (MQ=255)

                              aCGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGtt                                                                   <  2:7168910/101‑1 (MQ=255)

                                ggCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCa                                                                 <  2:7168621/101‑1 (MQ=255)

                                 gCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTGCACCGCATGGGTTCAt                                                                >  2:7169187/1‑101 (MQ=255)

                                  cAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATc                                                               >  1:7169190/1‑101 (MQ=255)

                                  cAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATc                                                               <  2:7169012/101‑1 (MQ=255)

                                   aaCGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCg                                                              <  1:7168973/101‑1 (MQ=255)

                                   aaCGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCg                                                              <  2:7169046/101‑1 (MQ=255)

                                    aCGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCgg                                                             <  1:7168713/101‑1 (MQ=255)

                                      gCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGgcg                                                           >  1:7169195/1‑101 (MQ=255)

                                         aaTGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCtt                                                        >  2:7169199/1‑101 (MQ=255)

                                         aaTGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCtt                                                        <  2:7168645/101‑1 (MQ=255)

                                          aTGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTc                                                       <  2:7168735/101‑1 (MQ=255)

                                           tGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCa                                                      <  1:7168936/101‑1 (MQ=255)

                                            ggTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCAc                                                     <  2:7168873/101‑1 (MQ=255)

                                             gTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCAcc                                                    <  1:7168798/101‑1 (MQ=255)

                                             gTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCAcc                                                    <  2:7168904/101‑1 (MQ=255)

                                             gTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCAcc                                                    <  1:7168653/101‑1 (MQ=255)

                                              tAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCa                                                   >  1:7169202/1‑101 (MQ=255)

                                               aGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCaa                                                  >  2:7169203/1‑101 (MQ=255)

                                                  aGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATcc                                               >  1:7169208/1‑101 (MQ=255)

                                                    cTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTa                                                                                          >  2:7169210/1‑56 (MQ=255)

                                                      gCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCAt                                           >  2:7169216/1‑101 (MQ=255)

                                                      gCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCAt                                           >  2:7169214/1‑101 (MQ=255)

                                                        gTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATAc                                         >  1:7169217/1‑101 (MQ=255)

                                                         ttGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATAcc                                        <  2:7168592/101‑1 (MQ=255)

                                                         ttGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATAcc                                        <  2:7169115/101‑1 (MQ=255)

                                                         ttGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATAcc                                        <  1:7168912/101‑1 (MQ=255)

                                                         ttGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATAcc                                        >  1:7169219/1‑101 (MQ=255)

                                                         ttGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATAcc                                        <  2:7168805/101‑1 (MQ=255)

                                                          tGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCg                                       >  1:7169222/1‑101 (MQ=255)

                                                            aagaagCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGAt                                     >  2:7169227/1‑101 (MQ=255)

                                                             agaagCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATa                                    <  1:7168690/101‑1 (MQ=255)

                                                              gaagCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATAc                                   <  1:7168771/101‑1 (MQ=255)

                                                              gaagCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATAc                                   <  1:7168819/101‑1 (MQ=255)

                                                                agCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGc                                 >  1:7169231/1‑101 (MQ=255)

                                                                agCCCATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGc                                 >  2:7169230/1‑101 (MQ=255)

                                                                   ccATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCAcc                              >  1:7169235/1‑101 (MQ=255)

                                                                   ccATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCAcc                              <  2:7168979/101‑1 (MQ=255)

                                                                    cATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCa                             <  2:7168796/101‑1 (MQ=255)

                                                                    cATCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCa                             >  2:7169238/1‑101 (MQ=255)

                                                                      tCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAac                           <  2:7168860/101‑1 (MQ=255)

                                                                      tCGGGCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAac                           <  1:7168759/101‑1 (MQ=255)

                                                                        gggCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAacac                         <  2:7168749/101‑1 (MQ=255)

                                                                        gggCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAacac                         >  2:7169242/1‑101 (MQ=255)

                                                                        gggCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAacac                         <  1:7168839/101‑1 (MQ=255)

                                                                         ggCGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACg                        >  1:7169244/1‑101 (MQ=255)

                                                                           cGTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGaa                      <  2:7168852/101‑1 (MQ=255)

                                                                            gTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAAc                     >  2:7169250/1‑101 (MQ=255)

                                                                            gTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAAc                     >  1:7169248/1‑101 (MQ=255)

                                                                            gTCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAAc                     >  1:7169251/1‑101 (MQ=255)

                                                                             tCCGCGCTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACt                    >  1:7169255/1‑101 (MQ=255)

                                                                                gcgcTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCg                 <  1:7168882/101‑1 (MQ=255)

                                                                                gcgcTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCg                 >  2:7169258/1‑101 (MQ=255)

                                                                                 cgcTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGt                >  2:7169261/1‑101 (MQ=255)

                                                                                  gcTCTTTGGTGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTa               >  1:7169262/1‑101 (MQ=255)

                                                                                  gcTCTTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTa               <  2:7168949/101‑1 (MQ=255)

                                                                                   ctctTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTaa              >  1:7169266/1‑101 (MQ=255)

                                                                                    tctTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTaaa             >  2:7169268/1‑101 (MQ=255)

                                                                                    tctTTGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTaaa             <  2:7168878/101‑1 (MQ=255)

                                                                                      tttGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGt           >  2:7169272/1‑101 (MQ=255)

                                                                                       ttGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGtt          >  1:7169275/1‑101 (MQ=255)

                                                                                       ttGATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGtt          <  1:7168800/101‑1 (MQ=255)

                                                                                         gATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTtgt        >  1:7169279/1‑101 (MQ=255)

                                                                                         gATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTtgt        <  1:7169070/101‑1 (MQ=255)

                                                                                         gATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTtgt        <  1:7169011/101‑1 (MQ=255)

                                                                                         gATGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTtgt        <  1:7168666/101‑1 (MQ=255)

                                                                                          aTGTTGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTtgtg       >  2:7169281/1‑101 (MQ=255)

                                                                                             ttGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCAc    >  2:7169284/1‑101 (MQ=255)

                                                                                             ttGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCAc    <  1:7168862/101‑1 (MQ=255)

                                                                                             ttGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCAc    <  1:7168783/101‑1 (MQ=255)

                                                                                              tGAATCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCAcc   >  1:7169288/1‑101 (MQ=255)

AGCATCATCGCCGCCAGAATCGGTGGGCTTACGGCAACGCCAATGGTAGAAGCTGCGTTGAAGAAGCCCATCGGGCGTCCGCGCTCTTTGATGTTGAACCAGTCGTTAATGACTTTTACACCGCATGGGTTCATCGGCGCTTCACCAATCCCCATACCGATACGCACCAACACGAACTGCGTAAAGTTGTGCACCA  >  NC_000913/4594151‑4594346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`