BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,731	C→T	A264A (GCG→GCA)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,731	0	C	T	100.0%	1027.1 / NA	243	A264A (GCG→GCA)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (122/121); total (122/121)

Only 100 of 247 total aligned reads displayed.

AGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAA  >  NC_000913/4629633‑4629828

    cttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGct                                                                                             >  2:7222453/1‑101 (MQ=255)

     ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGctt                                                                                            >  1:7222454/1‑101 (MQ=255)

      tcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGcttc                                                                                           >  2:7222457/1‑101 (MQ=255)

         aaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTtg                                                                                        <  1:7222098/101‑1 (MQ=255)

          aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTtgt                                                                                       >  1:7222461/1‑101 (MQ=255)

           gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTtgtg                                                                                      <  1:7221797/101‑1 (MQ=255)

            cgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGa                                                                                     <  1:7221890/101‑1 (MQ=255)

            cgagcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGtgcc                                                                                                >  2:7222462/4‑90 (MQ=255)

             gcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGaa                                                                                    <  2:7221947/101‑1 (MQ=255)

              cgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAg                                                                                   >  2:7222465/1‑101 (MQ=255)

              cgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAg                                                                                   >  1:7222467/1‑101 (MQ=255)

                ccAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCt                                                                                 >  1:7222470/1‑101 (MQ=255)

                 cAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCtt                                                                                >  2:7222471/1‑101 (MQ=255)

                  aGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTc                                                                               >  2:7222474/1‑101 (MQ=255)

                     cGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTg                                                                            <  2:7222100/101‑1 (MQ=255)

                       tGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTgcg                                                                          >  2:7222479/1‑101 (MQ=255)

                       tGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTgcg                                                                          <  1:7222223/101‑1 (MQ=255)

                        gCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTgcgc                                                                         >  1:7222480/1‑101 (MQ=255)

                          tttACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCa                                                                       >  2:7222484/1‑101 (MQ=255)

                           ttACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAg                                                                      <  1:7222121/101‑1 (MQ=255)

                           ttACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAg                                                                      >  1:7222485/1‑101 (MQ=255)

                              ccTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                                                   <  2:7222275/101‑1 (MQ=255)

                               cTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcgc                                                                  >  1:7222490/1‑101 (MQ=255)

                                tttCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgc                                                                    >  2:7222494/1‑98 (MQ=255)

                                tttCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                                                 >  1:7222492/1‑101 (MQ=255)

                                tttCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                                                 <  1:7222250/101‑1 (MQ=255)

                                   cGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAgg                                                                     >  2:7222498/1‑94 (MQ=255)

                                   cGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGAt                                                              <  1:7222139/101‑1 (MQ=255)

                                   cGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACATGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGAt                                                              <  2:7222193/101‑1 (MQ=255)

                                    gACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATc                                                             >  1:7222499/1‑101 (MQ=255)

                                      cTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCtt                                                           <  2:7222152/101‑1 (MQ=255)

                                       tGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                                                          <  2:7222061/101‑1 (MQ=255)

                                       tGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                                                          >  2:7222502/1‑101 (MQ=255)

                                        gACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                                                         <  1:7221989/101‑1 (MQ=255)

                                         aCGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCt                                                        <  1:7222045/101‑1 (MQ=255)

                                          cGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTg                                                       >  2:7222507/1‑101 (MQ=255)

                                          cGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTg                                                       <  1:7221942/101‑1 (MQ=255)

                                            gCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGa                                                               <  1:7222200/91‑1 (MQ=255)

                                            gCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCt                                                     <  2:7222254/101‑1 (MQ=255)

                                             ccTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTc                                                    <  1:7222063/101‑1 (MQ=255)

                                             ccTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTc                                                    <  2:7221972/101‑1 (MQ=255)

                                             ccTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTc                                                    <  2:7222338/101‑1 (MQ=255)

                                              cTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTcc                                                   >  2:7222509/1‑101 (MQ=255)

                                              cTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTcc                                                   <  2:7222103/101‑1 (MQ=255)

                                              cTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTcc                                                   >  1:7222510/1‑101 (MQ=255)

                                               tttAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTcca                                                  <  2:7222126/101‑1 (MQ=255)

                                               tttAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTcca                                                  <  2:7222201/101‑1 (MQ=255)

                                                ttAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccag                                                 <  1:7222187/101‑1 (MQ=255)

                                                ttAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccag                                                 <  2:7221796/101‑1 (MQ=255)

                                                  aGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcc                                               <  2:7222138/101‑1 (MQ=255)

                                                   gTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcca                                              >  2:7222514/1‑101 (MQ=255)

                                                   gTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcca                                              >  1:7222515/1‑101 (MQ=255)

                                                   gTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcca                                              <  1:7222117/101‑1 (MQ=255)

                                                    tACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGCTc                                                             >  2:7222517/1‑85 (MQ=255)

                                                      ccTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagga                                           >  1:7222520/1‑101 (MQ=255)

                                                       cTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggag                                          <  1:7222090/101‑1 (MQ=255)

                                                       cTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggag                                          >  1:7222524/1‑101 (MQ=255)

                                                        ttGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg                                         >  2:7222526/1‑101 (MQ=255)

                                                         tGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAggagga                                        >  1:7222528/1‑101 (MQ=255)

                                                          gACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag                                       <  2:7222075/101‑1 (MQ=255)

                                                             gTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagtag                                    <  2:7222051/101‑1 (MQ=255)

                                                                cccATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagtattaa                                 <  2:7222159/101‑5 (MQ=255)

                                                                cccATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTa                                 <  2:7222035/101‑1 (MQ=255)

                                                                cccATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTa                                 >  2:7222531/1‑101 (MQ=255)

                                                                  cATTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGtt                                  <  2:7222115/98‑1 (MQ=255)

                                                                   aTTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagtag                                    <  2:7222056/95‑1 (MQ=255)

                                                                   aTTCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCt                              >  1:7222533/1‑101 (MQ=255)

                                                                     tCCAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTc                            >  2:7222536/1‑101 (MQ=255)

                                                                      ccAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTcc                           <  2:7222079/101‑1 (MQ=255)

                                                                      ccAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTcc                           <  1:7222160/101‑1 (MQ=255)

                                                                       cAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTccc                          <  2:7222111/101‑1 (MQ=255)

                                                                       cAGCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTccc                          >  1:7222537/1‑101 (MQ=255)

                                                                         gCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCAc                        <  1:7222174/101‑1 (MQ=255)

                                                                         gCTCTTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCAc                        <  1:7222007/101‑1 (MQ=255)

                                                                           tctTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACgg                      >  2:7222540/1‑101 (MQ=255)

                                                                           tctTTCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACgg                      <  1:7222146/101‑1 (MQ=255)

                                                                             tttCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagta                                     >  1:7222543/1‑84 (MQ=255)

                                                                              ttCTCAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAAt                   >  2:7222545/1‑101 (MQ=255)

                                                                               tctcAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagta                                     >  1:7222547/1‑82 (MQ=255)

                                                                                  cAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCt               >  1:7222552/1‑101 (MQ=255)

                                                                                  cAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCt               >  2:7222551/1‑101 (MQ=255)

                                                                                  cAATCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCt               <  1:7222306/101‑1 (MQ=255)

                                                                                   aaTCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCtt              >  1:7222554/1‑101 (MQ=255)

                                                                                   aaTCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCtt              <  2:7222228/101‑1 (MQ=255)

                                                                                    aTCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTc             >  1:7222558/1‑101 (MQ=255)

                                                                                     tCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACg                       <  2:7221936/90‑1 (MQ=255)

                                                                                     tCGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCa            <  2:7222279/101‑1 (MQ=255)

                                                                                      cGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCAc           <  2:7222189/101‑1 (MQ=255)

                                                                                      cGACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCAc           >  2:7222561/1‑101 (MQ=255)

                                                                                       gACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCAcc          >  2:7222565/1‑101 (MQ=255)

                                                                                       gACTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCAcc          >  1:7222563/1‑101 (MQ=255)

                                                                                        aCTTACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACcg         <  2:7222307/101‑1 (MQ=255)

                                                                                          ttACGACGTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACcgcg       >  1:7222568/1‑101 (MQ=255)

                                                                                            acgacgTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGt     <  1:7222379/101‑1 (MQ=255)

                                                                                            acgacgTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGt     >  2:7222571/1‑101 (MQ=255)

                                                                                            acgacgTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGt     >  1:7222572/1‑101 (MQ=255)

                                                                                             cgacgTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTc    <  1:7222330/101‑1 (MQ=255)

                                                                                              gacgTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACcgcg       >  2:7222576/1‑97 (MQ=255)

                                                                                              gacgTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCa   <  1:7222503/101‑1 (MQ=255)

                                                                                               acgTGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCaa  >  1:7222577/1‑101 (MQ=255)

AGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAA  >  NC_000913/4629633‑4629828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`