BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,854	A→G	D231G (GAC→GGC)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,854	0	A	G	100.0%	1317.8 / NA	320	D231G (GAC→GGC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (127/193); total (127/193)

Only 100 of 320 total aligned reads displayed.

TACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAA  >  NC_000913/12756‑12951

tACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCt                                                                                                 >  1:20591/1‑101 (MQ=255)

   tttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCg                                                                                              <  2:20237/101‑1 (MQ=255)

    ttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGa                                                                                             >  1:20601/1‑101 (MQ=255)

     tCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGAc                                                                                            <  2:20124/101‑1 (MQ=255)

      cGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCg                                                                                              >  2:20605/1‑98 (MQ=255)

       gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAg                                                                                          >  1:20609/1‑101 (MQ=255)

       gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAg                                                                                          <  2:20174/101‑1 (MQ=255)

       gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAg                                                                                          <  2:20259/101‑1 (MQ=255)

        atatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGc                                                                                         <  2:19832/101‑1 (MQ=255)

         tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGcc                                                                                        >  2:20613/1‑101 (MQ=255)

           tttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGt                                                                                      >  1:20615/1‑101 (MQ=255)

             tCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCt                                                                                    <  1:19631/101‑1 (MQ=255)

             tCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCt                                                                                    >  2:20618/1‑101 (MQ=255)

             tCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCt                                                                                    <  1:19967/101‑1 (MQ=255)

               tattatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGa                                                                                  <  1:20151/101‑1 (MQ=255)

                attatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGAt                                                                                 <  2:20216/101‑1 (MQ=255)

                 ttatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATc                                                                                >  1:20624/1‑101 (MQ=255)

                    tCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAAc                                                                             <  2:19999/101‑1 (MQ=255)

                     cGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACt                                                                            <  2:20368/101‑1 (MQ=255)

                      gAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTa                                                                           >  1:20629/1‑101 (MQ=255)

                      gAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTa                                                                           >  2:20630/1‑101 (MQ=255)

                        aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATc                                                                         >  1:20637/1‑101 (MQ=255)

                         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCt                                                                        >  1:20641/1‑101 (MQ=255)

                         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCt                                                                        <  1:20243/101‑1 (MQ=255)

                         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCt                                                                        >  2:20642/1‑101 (MQ=255)

                         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCt                                                                        <  2:20469/101‑1 (MQ=255)

                          tCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTg                                                                       <  1:20001/101‑1 (MQ=255)

                           cgacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTgg                                                                      <  2:20302/101‑1 (MQ=255)

                           cgacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTgg                                                                      <  1:20261/101‑1 (MQ=255)

                            gacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGt                                                                     <  1:20270/101‑1 (MQ=255)

                             acgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGtt                                                                    >  1:20647/1‑101 (MQ=255)

                              cgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTg                                                                   <  1:20102/101‑1 (MQ=255)

                               gaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTga                                                                  >  1:20650/1‑101 (MQ=255)

                                aaGTTGACGGCGAAAAATCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTgaa                                                                 <  1:20120/101‑1 (MQ=255)

                                aaGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTgaa                                                                 >  2:20653/1‑101 (MQ=255)

                                  gTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTgaaga                                                               <  1:20233/101‑1 (MQ=255)

                                   ttGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTgaagaa                                                              <  1:20191/101‑1 (MQ=255)

                                   ttGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTgaagaa                                                              >  1:20658/1‑101 (MQ=255)

                                   ttGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTgaagaa                                                              <  2:20101/101‑1 (MQ=255)

                                     gACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAAtt                                                            >  2:20659/1‑101 (MQ=255)

                                     gACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAAtt                                                            <  1:20027/101‑1 (MQ=255)

                                        ggCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCaa                                                         >  1:20664/1‑101 (MQ=255)

                                          cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCaaga                                                       >  2:20667/1‑101 (MQ=255)

                                           gAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCaagaa                                                      >  1:20669/1‑101 (MQ=255)

                                            aaaaaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCaagaaa                                                     >  1:20672/1‑101 (MQ=255)

                                            aaaaaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCaagaaa                                                     <  2:20149/101‑1 (MQ=255)

                                             aaaaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCaagaaag                                                    <  2:20496/101‑1 (MQ=255)

                                                aaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATc                                                 >  2:20677/1‑101 (MQ=255)

                                                 aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCa                                                <  2:20029/101‑1 (MQ=255)

                                                 aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCa                                                >  1:20679/1‑101 (MQ=255)

                                                    ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCa                                                >  1:20683/1‑98 (MQ=255)

                                                    ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAggg                                             <  2:20061/101‑1 (MQ=255)

                                                       gAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCAt                                          <  1:20160/101‑1 (MQ=255)

                                                       gAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCAt                                          >  2:20684/1‑101 (MQ=255)

                                                        aaGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCAtt                                         <  2:19963/101‑1 (MQ=255)

                                                        aaGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCAtt                                         <  1:20112/101‑1 (MQ=255)

                                                         aGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTg                                        <  2:20249/101‑1 (MQ=255)

                                                          gTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGa                                       >  1:20689/1‑101 (MQ=255)

                                                            tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGt                                                                     <  1:20284/69‑1 (MQ=255)

                                                            tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGAcc                                     <  1:20077/101‑1 (MQ=255)

                                                            tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGAcc                                     <  1:20200/101‑1 (MQ=255)

                                                              tGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTg                                   <  2:20164/101‑1 (MQ=255)

                                                              tGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTg                                   <  1:20319/101‑1 (MQ=255)

                                                              tGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTg                                   >  2:20694/1‑101 (MQ=255)

                                                               ggCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTgc                                  <  1:20414/101‑1 (MQ=255)

                                                                gCAACCAACGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTgcg                                 <  2:20108/101‑1 (MQ=255)

                                                                 cacccaacGGTGATCCCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCt                                                                        <  2:19864/58‑1 (MQ=255)

                                                                 caaccaacGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCaagaa                                                      <  2:20228/79‑1 (MQ=255)

                                                                 caaccaacGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTgcgc                                >  1:20695/1‑101 (MQ=255)

                                                                 caaccaacGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTgcgc                                <  2:20090/101‑1 (MQ=255)

                                                                    ccaacGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAAc                             <  2:20045/101‑1 (MQ=255)

                                                                     caacGGTGATACCCACCTGGGGGGTGAAGGCTTCGAc                                                                                            >  1:20699/1‑37 (MQ=255)

                                                                     caacGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACg                            <  2:20201/101‑1 (MQ=255)

                                                                       acGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGAt                          <  1:20332/101‑1 (MQ=255)

                                                                       acGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGAt                          >  2:20702/1‑101 (MQ=255)

                                                                       acGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGAt                          <  2:19934/101‑1 (MQ=255)

                                                                       acGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGAt                          <  1:20132/101‑1 (MQ=255)

                                                                        cGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATc                         <  2:20333/101‑1 (MQ=255)

                                                                        cGGTGATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATc                         >  1:20704/1‑101 (MQ=255)

                                                                            gATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCt                     <  1:20477/101‑1 (MQ=255)

                                                                            gATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCt                     >  1:20707/1‑101 (MQ=255)

                                                                            gATACCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCt                     <  1:19903/101‑1 (MQ=255)

                                                                               aCCCACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGc                  >  2:20712/1‑101 (MQ=255)

                                                                                cccACCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTg                    <  2:20271/98‑1 (MQ=255)

                                                                                   aCCTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATg              >  2:20716/1‑101 (MQ=255)

                                                                                    ccTGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGc             >  1:20718/1‑101 (MQ=255)

                                                                                      tGGGGGGTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAg           <  1:20543/101‑1 (MQ=255)

                                                                                       ggggggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCTATGCAgc          <  1:20223/101‑1 (MQ=255)

                                                                                       ggggggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgc          <  2:20209/101‑1 (MQ=255)

                                                                                       ggggggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgc          <  1:20311/101‑1 (MQ=255)

                                                                                       ggggggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgc          <  1:20178/101‑1 (MQ=255)

                                                                                         ggggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc        >  1:20722/1‑101 (MQ=255)

                                                                                         ggggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc        <  1:20370/101‑1 (MQ=255)

                                                                                          gggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgc          <  1:20218/98‑1 (MQ=255)

                                                                                          gggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGcc       >  2:20723/1‑101 (MQ=255)

                                                                                           ggTGAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCt      <  2:20385/101‑1 (MQ=255)

                                                                                            gttaaGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa            <  2:20179/91‑1 (MQ=255)

                                                                                              gAAGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaa   <  2:20137/101‑1 (MQ=255)

                                                                                               aaGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaaa  <  2:20292/101‑1 (MQ=255)

                                                                                               aaGGCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaaa  >  1:20728/1‑101 (MQ=255)

TACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAA  >  NC_000913/12756‑12951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`