BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	282,008	T→C	intergenic (‑25/‑270)	yagA ← / → yagE	CP4‑6 prophage; integrase core domain‑containing protein YagA/CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	282,008	0	T	C	100.0%	1148.9 / NA	269	intergenic (‑25/‑270)	yagA/yagE	CP4‑6 prophage; integrase core domain‑containing protein YagA/CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (165/104); total (165/104)

Only 100 of 269 total aligned reads displayed.

TGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTC  >  NC_000913/281910‑282105

tGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCaa                                                                                                 <  2:440537/101‑1 (MQ=255)

  cGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAgt                                                                                               >  1:441079/1‑101 (MQ=255)

  cGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAgt                                                                                               >  2:441077/1‑101 (MQ=255)

    aGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAgtgt                                                                                             <  1:440541/101‑1 (MQ=255)

     ggCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTc                                                                                            >  1:441088/1‑101 (MQ=255)

     ggCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTc                                                                                            <  2:440754/101‑1 (MQ=255)

     ggCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTc                                                                                            <  1:440699/101‑1 (MQ=255)

     ggCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTc                                                                                            >  2:441086/1‑101 (MQ=255)

       cGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTa                                                                                          <  1:440677/101‑1 (MQ=255)

       cGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTa                                                                                          <  2:440560/101‑1 (MQ=255)

         aacaaaacaaACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTAcc                                                                                        >  2:441093/1‑101 (MQ=255)

         aacaaaacaaACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTAcc                                                                                        >  1:441090/1‑101 (MQ=255)

          acaaaacaaaCTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCa                                                                                       >  2:441097/1‑101 (MQ=255)

          acaaaacaaaCTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCa                                                                                       >  1:441096/1‑101 (MQ=255)

           caaaacaaaCTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCAt                                                                                      <  1:440236/101‑1 (MQ=255)

             aaacaaacTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGt                                                                                    >  1:441101/1‑101 (MQ=255)

                 aaacTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTcccc                                                                                >  1:441105/1‑101 (MQ=255)

                 aaacTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTcccc                                                                                >  2:441104/1‑101 (MQ=255)

                  aacTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCg                                                                               >  1:441109/1‑101 (MQ=255)

                    cTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGaa                                                                             <  1:440390/101‑1 (MQ=255)

                     tCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAAc                                                                            <  1:440690/101‑1 (MQ=255)

                     tCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAAc                                                                            <  1:440635/101‑1 (MQ=255)

                      cAGTACGTAATGACATGGTATCTCTCGCATCCCATGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACa                                                                           >  2:441113/1‑101 (MQ=255)

                      cAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACa                                                                           <  2:440556/101‑1 (MQ=255)

                       aGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACaa                                                                          >  1:441114/1‑101 (MQ=255)

                        gTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAg                                                                         >  1:441117/1‑101 (MQ=255)

                         tACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAgt                                                                        <  2:440590/101‑1 (MQ=255)

                         tACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAgt                                                                        >  2:441120/1‑101 (MQ=255)

                          aCGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAgtg                                                                       >  1:441122/1‑101 (MQ=255)

                           cGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTCCCATGTCCCCGAACAAgtgt                                                                      <  2:440723/101‑1 (MQ=255)

                           cGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCTTGTCCCCGAACAAgtgt                                                                      <  1:440724/101‑1 (MQ=255)

                            gTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGtt                                                                     >  1:441125/1‑101 (MQ=255)

                              aaTGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCa                                                                   >  1:441128/1‑101 (MQ=255)

                               aTGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCAc                                                                  >  1:441132/1‑101 (MQ=255)

                                  aCATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTAt                                                               <  1:440791/101‑1 (MQ=255)

                                   cATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATg                                                              <  2:440805/101‑1 (MQ=255)

                                   cATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATg                                                              >  2:441134/1‑101 (MQ=255)

                                    aTGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGt                                                             <  2:440828/101‑1 (MQ=255)

                                     tGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTc                                                            >  1:441138/1‑101 (MQ=255)

                                      ggTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTcc                                                           <  2:440705/101‑1 (MQ=255)

                                      ggTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTcc                                                           >  2:441139/1‑101 (MQ=255)

                                        tATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTcccc                                                         >  2:441144/1‑101 (MQ=255)

                                        tATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTcccc                                                         >  1:441143/1‑101 (MQ=255)

                                         aTCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACaa                                                                          >  1:441146/1‑83 (MQ=255)

                                         aTCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCg                                                        <  1:440837/101‑1 (MQ=255)

                                           ctctcGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGa                                                      <  2:440632/101‑1 (MQ=255)

                                           ctctcGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGa                                                      >  2:441149/1‑101 (MQ=255)

                                            tctcGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGAc                                                     <  2:440445/101‑1 (MQ=255)

                                            tctcGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGAc                                                     >  1:441153/1‑101 (MQ=255)

                                             ctcGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGAcc                                                    <  2:440626/101‑1 (MQ=255)

                                             ctcGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGAcc                                                    <  2:440901/101‑1 (MQ=255)

                                              tcGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCg                                                   >  1:441157/1‑101 (MQ=255)

                                                 cATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGCac                                                >  2:441158/1‑101 (MQ=255)

                                                  aTCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTaca                                               >  1:441161/1‑101 (MQ=255)

                                                     ccAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACAccc                                            >  2:441166/1‑101 (MQ=255)

                                                     ccAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACAccc                                            >  1:441165/1‑101 (MQ=255)

                                                       aGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCa                                          >  2:441168/1‑101 (MQ=255)

                                                       aGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCa                                          >  2:441172/1‑101 (MQ=255)

                                                        gggCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCaa                                         >  1:441174/1‑101 (MQ=255)

                                                          gCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAg                                       <  1:440916/101‑1 (MQ=255)

                                                          gCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAg                                       >  1:441176/1‑101 (MQ=255)

                                                            aTAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAggg                                     <  2:440871/101‑1 (MQ=255)

                                                            aTAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAggg                                     >  1:441181/1‑101 (MQ=255)

                                                            aTAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAggg                                     >  2:441183/1‑101 (MQ=255)

                                                              aaGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGga                                   >  1:441188/1‑101 (MQ=255)

                                                              aaGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGga                                   >  1:441186/1‑101 (MQ=255)

                                                               aGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGgag                                  <  2:440466/101‑1 (MQ=255)

                                                               aGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGgag                                  >  2:441189/1‑101 (MQ=255)

                                                                 cGACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAggg                                     >  2:441194/1‑96 (MQ=255)

                                                                  gACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTGTGTCCCCGGACCGTACACCCCAAAGGGGAGAgg                               >  2:441198/1‑101 (MQ=255)

                                                                  gACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAgg                               <  2:440701/101‑1 (MQ=255)

                                                                  gACTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAgg                               >  1:441196/1‑101 (MQ=255)

                                                                   aCTCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAggg                              <  2:440775/101‑1 (MQ=255)

                                                                     tCCATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGa                            >  2:441200/1‑101 (MQ=255)

                                                                      ccATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGAc                           <  2:440653/101‑1 (MQ=255)

                                                                       cATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGgag                                  <  1:440808/93‑1 (MQ=255)

                                                                       cATAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACt                          >  1:441204/1‑101 (MQ=255)

                                                                         tAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGc                        >  2:441207/1‑101 (MQ=255)

                                                                         tAAACGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGc                        >  1:441209/1‑101 (MQ=255)

                                                                          aaaCGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCa                       <  1:440776/101‑1 (MQ=255)

                                                                            aCGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCAcc                     <  1:440814/101‑1 (MQ=255)

                                                                             cGGGTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCg                    >  1:441212/1‑101 (MQ=255)

                                                                                gTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGc                 <  2:440819/101‑1 (MQ=255)

                                                                                gTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGc                 >  1:441217/1‑101 (MQ=255)

                                                                                gTTCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGc                 >  2:441216/1‑101 (MQ=255)

                                                                                 ttCTTATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGcc                <  1:440858/101‑1 (MQ=255)

                                                                                    ttATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATc             >  2:441221/1‑101 (MQ=255)

                                                                                    ttATGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATc             <  1:440593/101‑1 (MQ=255)

                                                                                      aTGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCtt           >  1:441224/1‑101 (MQ=255)

                                                                                      aTGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCtt           <  1:440752/101‑1 (MQ=255)

                                                                                       tGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCttt          >  2:441226/1‑101 (MQ=255)

                                                                                       tGCCTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCttt          >  1:441227/1‑101 (MQ=255)

                                                                                         ccTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTc        >  1:441231/1‑101 (MQ=255)

                                                                                         ccTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTc        <  2:440484/101‑1 (MQ=255)

                                                                                          cTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTcc       >  1:441236/1‑101 (MQ=255)

                                                                                          cTTAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTcc       >  1:441234/1‑101 (MQ=255)

                                                                                           ttAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTccc      <  1:440988/101‑1 (MQ=255)

                                                                                            tAGTTGCAAGTGTCTACGATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTcccc     >  2:441239/1‑101 (MQ=255)

                                                                                            tAGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCttt          <  2:440961/96‑1 (MQ=255)

                                                                                             aGTTGCAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTct       >  1:441242/1‑97 (MQ=255)

TGCGAGGCGAACAAAACAAACTCAGTACGTAATGACATGGTATCTCTCGCATCCCAGGGCATAAGCGACTCCATAAACGGGTTCTTATGCCTTAGTTGTAAGTGTCTACCATGTCCCCGAACAAGTGTTCACTATGTCCCCGGACCGTACACCCCAAAGGGGAGAGGGGACTGCACCGAGCCATCTTTTCCCCCTC  >  NC_000913/281910‑282105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`